Title:
A molecule complex used for a change method of a genome sequence and it which change a nucleic acid base of targeted DNA arrangement specifically
Document Type and Number:
Japanese Patent JP6206893
Kind Code:
B2
Abstract:
The present invention provides a method that modifies a targeted region of double-stranded DNA, the method including a step wherein selected double-stranded DNA is brought into contact with a complex wherein a nucleic acid base conversion enzyme is bound to a nucleic acid sequence recognition module that specifically binds to a target nucleotide sequence within the double-stranded DNA and, without severing either strand of the double-stranded DNA at the targeted region, one or more nucleotides in the targeted region are converted into one or more other nucleotides or are deleted, or one or more nucleotides are inserted at the targeted region.
Inventors:
Keiji Nishida
Akihiko Kondo
Satomi Kojima
Akihiko Kondo
Satomi Kojima
Application Number:
JP2016506541A
Publication Date:
October 04, 2017
Filing Date:
March 04, 2015
Export Citation:
Assignee:
National University Corporation Kobe University
International Classes:
C12N15/09; C12N9/16; C12N9/78
Foreign References:
| WO2015089406A1 | ||||
| WO2013176772A1 | ||||
| WO2011072246A1 | ||||
| WO2013140250A1 |
Other References:
Science, 2013, Vol.339, p.819-823
Science, 2013, Vol.339, p.823-826
Nature Biotechnology, 2013, Vol.31, p.230-232
Nature Biotechnology, 2013, Vol.31, p.233-239, online methods
eLIFE, 2013, Vol.2, e00471
Nucleic Acids Research, 2013, Vol.41, p.4336-4343
Science, 2013, Vol.339, p.823-826
Nature Biotechnology, 2013, Vol.31, p.230-232
Nature Biotechnology, 2013, Vol.31, p.233-239, online methods
eLIFE, 2013, Vol.2, e00471
Nucleic Acids Research, 2013, Vol.41, p.4336-4343
Attorney, Agent or Firm:
Takashima Ichi
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Junzo Koike
Hirofumi Toma
Atsuko Akai
Tomiya Tozaki
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Junzo Koike
Hirofumi Toma
Atsuko Akai
Tomiya Tozaki