新規なカルボニル還元酵素、その酵素をコードするDNAを含むポリヌクレオチド、その製造方法、およびこれを利用した光学活性アルコールの製造方法

Title:

Document Type and Number:

Japanese Patent JP4213524

Kind Code:

B2

Abstract:

An objective of the present invention is to provide methods for efficiently producing (S)-1-(3,4-dimethoxyphenyl)-2-propanol at a high optical purity. Another objective is to provide novel reductases which reduce 3,4-dimethoxyphenylacetone, using NADPH as a coenzyme, to produce (S) -1- (3, 4-dimethoxyphenyl) -2-propanol with a high optical purity. The inventors found that a 3,4-dimethoxyphenylacetone-reducing enzyme present in Torulaspora delbrueckii is a novel carbonyl reductase that reduces various carbonyls. This novel enzyme reduces 3,4-dimethoxyphenylacetone in a reduction reaction to produce (S)-1-(3,4-dimethoxyphenyl)-2-propanol with a high optical purity and at a high yield. Furthermore, the inventors isolated a DNA that encodes the present enzyme, and generated a recombinant bacterium which highly expresses the present enzyme. Thus, the present inventors established a simple and highly economical method of obtaining optically active alcohols with a high optical purity and at a high yield.

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Inventors:

Norihiro Kimoto
Hiroaki Yamamoto
Nakajima Kennori

Application Number:

JP2003163015A

Publication Date:

January 21, 2009

Filing Date:

June 06, 2003

Export Citation:

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Assignee:

Daicel Chemical Industry Co., Ltd.

International Classes:

C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N9/04; C12P7/02; C12P7/22

Domestic Patent References:

JP8325188A
JP8089261A

Foreign References:

WO1999023242A1

Attorney, Agent or Firm:

Hatsushi Shimizu

Previous Patent: JPS4213523

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