Title:
ビフィドバクテリウム由来の新規なプラスミド、これを利用した組換え発現ベクターおよび形質転換方法
Document Type and Number:
Japanese Patent JP2004519236
Kind Code:
A
Abstract:
The present invention relates to a new plasmid originated from (Bifidobacterium) a recombinant expression vector and transformation method using the same. More particularly, the present invention relates to a plasmid pMG1 having nucleotide sequence represented by SEQ.ID.NO.1; (Bifidobacterium longum) MG1 including the plasmid pMG1; and a shuttle vector which can be replicated in both (Bifidobacterium) and (E. coli), and comprises (Mob) gene having nucleotide sequence represented by SEQ.ID.NO.2. (Rep) gene having nucleotide sequence represented by SEQ.ID.NO.3 and a selection marker. The shuttle vector and the promoter of the present invention can be used for expressing target gene without additional purification process. The protein expressed from the target gene in (Bifidobacterium) can be added to food, therefore, the protein can be used for preparing food additives or oral vaccine, Furthermore, the potential and the possibilty of probiotics using (Bifidobacterium) can promoted through the development of the shuttle vector.
Inventors:
Gee, Geun-Aeog
Park, Myung-So
Kang, Yun-hee
Theo, Jun-Min
Park, Myung-So
Kang, Yun-hee
Theo, Jun-Min
Application Number:
JP2002568756A
Publication Date:
July 02, 2004
Filing Date:
March 30, 2001
Export Citation:
Assignee:
BIFIDO CO.,LTD
International Classes:
C12N1/21; C12N15/70; C12N15/09; C12N15/74; (IPC1-7): C12N15/09; C12N1/21
Attorney, Agent or Firm:
Shunsuke Nakao
Takashi Ito
Naoko Okura
Fumari Tamari
Takeyuki Suzuki
Takashi Ito
Naoko Okura
Fumari Tamari
Takeyuki Suzuki