Title:
オプソニン活性を有する新規な蛋白質
Document Type and Number:
Japanese Patent JP3571371
Kind Code:
B2
Abstract:
PURPOSE:To obtain the new protein derived from human plasma, having a specific amino acid sequence, presenting such opsonin activity as to be subject to oligophagous activity by oligophagous cells like neutrophiles through its sticking to pathogenic microbes, thus useful as a preventive/therapeutic agent for infectious diseases. CONSTITUTION:Calcium chloride is added to citric acid-added human plasma to effect coagulation followed by filtration and centrifugal separation to separate serum. Polyethylene glycol is then added to the serum and the resultant precipitate is centrifuged and then dissolved in a buffer solution. The resultant solution is passed through an yeast-immobilized mannan column which is then washed and treated with a mannose- contg. buffer solution, and the adsorbed substance is eluted using a 0.15 M N- acetylglucosamine-contg. buffer solution, and the impurities in the resultant elute is removed to purify the adsorbed substance, thus obtaining the objective new protein containing an amino acid sequence of formula I and having the following characteristics: molecular weight determined by SDS-polyacrylamide gel electrophoresis under reduction: about 35kDa; having amino acid sequence of formula II at the N- terminal; and, presenting opsonin activity. This protein is useful as, e.g. a preventive/ therapeutic agent for infectious diseases.
Inventors:
Sadazo Fujita
Misao Matsushita
Yuichi Endo
Misao Matsushita
Yuichi Endo
Application Number:
JP17878794A
Publication Date:
September 29, 2004
Filing Date:
July 29, 1994
Export Citation:
Assignee:
Sadazo Fujita
International Classes:
C12N15/09; A61K38/00; A61P31/04; C07H21/04; C07K14/47; C07K16/00; (IPC1-7): C12N15/09; A61K38/00; A61P31/04; C07K14/47
Other References:
ICHJO,H et al.,Molecular Cloning and Characterization of Ficolin, a Multimeric Protein with Fibrinogen- and Collag ,J. Biol. Chem.,1993年 7月,Vol.268, No.19,pp.14505-14513
ANDERSEN,Q. et al.,Purification,Subunit Characterization and Ultrastructure of Three Soluble Bovine Lectins:,Scand. J. Immunol.,1992年 7月,Vol.36,pp.131-141
ANDERSEN,Q. et al.,Purification,Subunit Characterization and Ultrastructure of Three Soluble Bovine Lectins:,Scand. J. Immunol.,1992年 7月,Vol.36,pp.131-141
Attorney, Agent or Firm:
Takashima Ichi