Title:
A nucleated erythrocyte analysis system and a method
Document Type and Number:
Japanese Patent JP6029657
Kind Code:
B2
Abstract:
Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
Inventors:
Wu, Zeon
Coleman, Mary Lou
Lynn, Emily H
Bourg, Michael Earl
Batuka, Jiakomo
Coleman, Mary Lou
Lynn, Emily H
Bourg, Michael Earl
Batuka, Jiakomo
Application Number:
JP2014509319A
Publication Date:
November 24, 2016
Filing Date:
April 26, 2012
Export Citation:
Assignee:
ABBOTT LABORATORIES
International Classes:
G01N33/49; G01N21/64
Domestic Patent References:
| JP9508705A | ||||
| JP2008003062A | ||||
| JP2008500558A | ||||
| JP2938976B2 | ||||
| JP2003510557A | ||||
| JP2012525589A | ||||
| JP9508703A | ||||
| JP2014196958A |
Attorney, Agent or Firm:
Kawaguchi International Patent Office