Title:
核酸分析デバイス,核酸分析装置、及び核酸分析方法
Document Type and Number:
Japanese Patent JP5372876
Kind Code:
B2
Abstract:
In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample. The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.
Inventors:
Takayuki Ohara
Kazushige Imai
Toshiro Saito
Satoshi Takahashi
Kazushige Imai
Toshiro Saito
Satoshi Takahashi
Application Number:
JP2010202626A
Publication Date:
December 18, 2013
Filing Date:
September 10, 2010
Export Citation:
Assignee:
Hitachi High-Technologies Corporation
International Classes:
C12M1/00; C12N15/09; C12Q1/68; G01N21/64; G01N21/78
Domestic Patent References:
| JP2003009890A | ||||
| JP2002214142A | ||||
| JP2010172271A | ||||
| JP2009300241A | ||||
| JP2005062074A |
Attorney, Agent or Firm:
Manabu Inoue
Yuji Toda
Shigemi Iwasaki
Yuji Toda
Shigemi Iwasaki