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Title:
酢酸耐性に関与する遺伝子、該遺伝子を用いて育種された酢酸菌、及び該酢酸菌を用いた食酢の製造方法
Document Type and Number:
Japanese Patent JP4326967
Kind Code:
B2
Abstract:
The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.

Inventors:
Eiji Goto
Shigeru Nakano
Application Number:
JP2003576627A
Publication Date:
September 09, 2009
Filing Date:
March 12, 2003
Export Citation:
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Assignee:
Mizkan Group Headquarters
International Classes:
C12N15/09; C07K14/195; C12J1/04; C12N1/20; C12N1/21; C12N9/10; C12N15/54
Domestic Patent References:
JP2002364A
JP3219878A
JP60180581A
JP60009489A
Other References:
Hiroko Ikushiro, et al.,A Water-soluble Homodimeric Serine Palmitoyltransferase from Sphingomonas paucimobilis EY2395T Strain,The Journal of Biological Chemistry,2001年 5月25日,Vol.276, No.21,pp.18249-18256
Peter Steiner, et al.,Proteins Induced during Adaption of Acetobacter aceti to High Acetate Concentrations,Applied and Environmental Microbiology,2001年12月,Vol.67, No.12,pp.5474-5481
後藤英嗣ほか,高酸性度および中酸性度食酢生産性の酢酸菌に含まれるリン脂質クラスの比較分析,日本油化学会誌,2000年,第49巻 第4号,349-355頁
Gary M., et al.,Role for de Novo Sphingoid Base Biosynthesis in the Heat-induced Transient Cell Cycle Arrest of Saccharomyces cerevisiae,The Journal of Biological Chemistry,2001年 3月16日,Vol.276, No.11,pp.8574-8581
Attorney, Agent or Firm:
Matsumoto Kuki
Toda parent man