PURPOSE: To obtain the subject new compd. which has a marker at a desired position without using a spacer and which is useful to detect DNA, RNA and their fragments.
CONSTITUTION: This compd. is expressed by formula I, for example, N4-[5- fluorescein aminocarbonylmethyl]-2',3',5'-tri-O-acetylcytidine. In formula I, R1 is expressed by formula II or formula III (R4 is H, OH or the like; R5, R6 are H, R8 or the like; R7 is H, a nucleoside or the like; R8 is OH or protecting group) or the like; R2 is phosphor; R3 is H, a 1-18C alkyl or the like. The compd. is obtd. by reacting a compd. expressed by R1-NH2 with a compd. expressed by R9-CO-R10 (R9 is Cl, imidazole or the like; R10 is imidazole, benztriazole or the like) in an org. solvent such as dioxane at 20 to 40°C, then reacting the product with an aromatic amine expressed by R2-NH2, for example, under same conditions above described, further introducing an R3 group, and directly removing the protecting group.
JPH07101976 | CARBINOSTATIC AGENT |
WO/2002/057425 | NUCLEOSIDE DERIVATIVES AS INHIBITORS OF RNA-DEPENDENT RNA VIRAL POLYMERASE |
WO/1998/028318 | THERAPEUTIC COMPOUNDS |
HARARUTO SHIGUMUNTO
BERUNTO RETSUKUMAN