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Title:
FLUORESCENT MARKER COMPOUND, ITS PREPARATION AND USE THEREOF
Document Type and Number:
Japanese Patent JPH0665280
Kind Code:
A
Abstract:

PURPOSE: To obtain the subject new compd. which has a marker at a desired position without using a spacer and which is useful to detect DNA, RNA and their fragments.

CONSTITUTION: This compd. is expressed by formula I, for example, N4-[5- fluorescein aminocarbonylmethyl]-2',3',5'-tri-O-acetylcytidine. In formula I, R1 is expressed by formula II or formula III (R4 is H, OH or the like; R5, R6 are H, R8 or the like; R7 is H, a nucleoside or the like; R8 is OH or protecting group) or the like; R2 is phosphor; R3 is H, a 1-18C alkyl or the like. The compd. is obtd. by reacting a compd. expressed by R1-NH2 with a compd. expressed by R9-CO-R10 (R9 is Cl, imidazole or the like; R10 is imidazole, benztriazole or the like) in an org. solvent such as dioxane at 20 to 40°C, then reacting the product with an aromatic amine expressed by R2-NH2, for example, under same conditions above described, further introducing an R3 group, and directly removing the protecting group.


Inventors:
BUORUFUGANKU PUFURAIDERAA
HARARUTO SHIGUMUNTO
BERUNTO RETSUKUMAN
Application Number:
JP9931493A
Publication Date:
March 08, 1994
Filing Date:
April 26, 1993
Export Citation:
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Assignee:
MERCK PATENT GMBH
International Classes:
C07H19/06; C07H19/04; C07H19/16; C07H21/00; C12Q1/68; G01N33/533; (IPC1-7): C07H19/06; C07H19/16; G01N33/533
Attorney, Agent or Firm:
Wakabayashi Tadashi