Title:
A reagent constituent for glycated hemoglobin measurement, and a glycated hemoglobin measuring method
Document Type and Number:
Japanese Patent JP6068916
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a glycosylated hemoglobin measuring method using an amadoriase, which can more easily and quickly obtain a measured value with high accuracy, and a reagent composition for measuring glycosylated hemoglobin which is used for the measuring method, has less restriction on use conditions, and enables sufficient exhibition of the performance of a prescribed enzyme.SOLUTION: A reagent composition for measuring glycosylated hemoglobin is formulated with an amadoriase whose reactivity against ε-fructosyl lysine to reactivity against α-fructosyl valyl histidine is remarkably reduced in a wide pH range from a weakly acidic region to a weakly alkaline region. A glycosylated hemoglobin measuring method uses the reagent composition. A measured value with high accuracy can be obtained in a wide pH range including a pH optimal for the reaction of the amadoriase without performing steps such as elimination of influential materials by pretreatment.
Inventors:
Atsushi Ichiyanagi
Yosuke Hake
Yosuke Hake
Application Number:
JP2012225189A
Publication Date:
January 25, 2017
Filing Date:
October 10, 2012
Export Citation:
Assignee:
Kikkoman Corporation
International Classes:
C12N15/09; C12N9/06; C12Q1/26; C12Q1/37
Domestic Patent References:
| JP2010233502A | ||||
| JP2011229526A | ||||
| JP2003235585A |
Foreign References:
| WO2012018094A1 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Takashima Ejima
Susumu Masuda
Satoshi Fujita
Natsuo Tanaka
Takashima Ejima
Susumu Masuda