Title:
A rough refining method of hydrophilic recombination protein
Document Type and Number:
Japanese Patent JP6077570
Kind Code:
B2
Abstract:
The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent.
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Inventors:
Toshiaki Osawa
Keisuke Morita
Keisuke Morita
Application Number:
JP2014554365A
Publication Date:
February 08, 2017
Filing Date:
December 18, 2013
Export Citation:
Assignee:
spiber corporation
International Classes:
C07K1/14; C12P21/02
Domestic Patent References:
| JP2011504374A | ||||
| JP2003509020A | ||||
| JP2012012332A |
Foreign References:
| WO2011133886A1 | ||||
| US4624756 |
Attorney, Agent or Firm:
Ikeuchi, Sato & Partners