Title:
A selection method of a human induced pluripotent stem cell
Document Type and Number:
Japanese Patent JP5936134
Kind Code:
B2
Abstract:
The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.
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Inventors:
Shinya Yamanaka
Takahashi Kazutori
Mari Onuki
Takahashi Kazutori
Mari Onuki
Application Number:
JP2012556312A
Publication Date:
June 15, 2016
Filing Date:
June 15, 2011
Export Citation:
Assignee:
Kyoto University
International Classes:
C12N5/10; C12N5/0793
Domestic Patent References:
| JP2010504090A |
Foreign References:
| WO2009148170A1 |
Other References:
MIURA,K. et al,Variation in the safety of induced pluripotent stem cell lines ,Nature biotechnology,2009年,Vol.27, No.8 ,p.743-5
YAMANAKA,S.,A fresh look at iPS cells ,Cell,2009年 4月 3日,Vol.137, No.1,pp.13-17
CHAMBERS SM et al,Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling ,Nature Biotechnology,2009年,Vol.27, No.3,p.275-80
HAO,J. et al,In Vivo Structure-Activity Relationship Study of Dorsomorphin Analogues Identifies Selective VEGF and BMP Inhibitors ,ACS Chemical Biology,2010年 2月,Vol.5, No.2,p.245-253
CUNY,G.D. et al,Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors,Bioorganic & medicinal chemistry letters ,2008年,Vol.18, No.15,p.4388-92
MINOWA,T. et al,Increase in tumor permeability following TGF-beta type I receptor-inhibitor treatment observed by dynamic contrast-enhanced MRI ,British journal of cancer,2009年,Vol.101, No.11 ,p.1884-90
TOJO,M. et al,The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta,Cancer science,2005年,Vol.96, No.11,p.791-800
EIRAKU M, et al.,Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals,Cell Stem Cell.,2008年,Vol.3, No. 5 ,p.519-32.
WATANABE K, et al.,A ROCK inhibitor permits survival of dissociated human embryonic stem cells,Nat Biotechnol. ,2007年,Vol.25, No.6,p.681-6.
WADA T, et al.,Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells ,PLoS One.,2009年,Vol.4, No.8,p.e6722
YAMANAKA,S.,A fresh look at iPS cells ,Cell,2009年 4月 3日,Vol.137, No.1,pp.13-17
CHAMBERS SM et al,Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling ,Nature Biotechnology,2009年,Vol.27, No.3,p.275-80
HAO,J. et al,In Vivo Structure-Activity Relationship Study of Dorsomorphin Analogues Identifies Selective VEGF and BMP Inhibitors ,ACS Chemical Biology,2010年 2月,Vol.5, No.2,p.245-253
CUNY,G.D. et al,Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors,Bioorganic & medicinal chemistry letters ,2008年,Vol.18, No.15,p.4388-92
MINOWA,T. et al,Increase in tumor permeability following TGF-beta type I receptor-inhibitor treatment observed by dynamic contrast-enhanced MRI ,British journal of cancer,2009年,Vol.101, No.11 ,p.1884-90
TOJO,M. et al,The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta,Cancer science,2005年,Vol.96, No.11,p.791-800
EIRAKU M, et al.,Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals,Cell Stem Cell.,2008年,Vol.3, No. 5 ,p.519-32.
WATANABE K, et al.,A ROCK inhibitor permits survival of dissociated human embryonic stem cells,Nat Biotechnol. ,2007年,Vol.25, No.6,p.681-6.
WADA T, et al.,Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells ,PLoS One.,2009年,Vol.4, No.8,p.e6722
Attorney, Agent or Firm:
Yoshiyuki Kawaguchi
Shinichi Sanuki
Takeshi Niwa
Shinichi Sanuki
Takeshi Niwa