Title:
A solid culture medium using the culture-medium material and this which consist of cellulose gel, and a cellulase activity assaying method using this culture medium
Document Type and Number:
Japanese Patent JP5907883
Kind Code:
B2
Abstract:
The present invention provides: a cellulose gel medium providing good visibility of microbial colonies, which improves on the weaknesses of the cellulose gel media of the prior art; a cellulose gel culture substrate for manufacturing the cellulose gel medium and a method for manufacturing the cellulose gel culture substrate; and a method for screening cellulase-producing micro-organisms or cellulase activity with greater efficiency and rapidity. One embodiment of the present invention is a culture substrate comprising a cellulose gel containing cellulose and water as medium-solidifying components, said cellulose characterized in that the viscosity at 26°C of a solution in which the cellulose has been dissolved at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (w/v) lithium chloride is 12 to 35 mPa·S.
Inventors:
Kazuki Nagaki
Yuki Kurosawa
Takuo Shiraishi
Exit Shigeru
Mikiko Tsuru
Yuki Kurosawa
Takuo Shiraishi
Exit Shigeru
Mikiko Tsuru
Application Number:
JP2012540839A
Publication Date:
April 26, 2016
Filing Date:
October 24, 2011
Export Citation:
Assignee:
Far East Pharmaceutical Industry Co., Ltd.
Japan Agency for Marine-Earth Science and Technology
Japan Agency for Marine-Earth Science and Technology
International Classes:
C12M1/00; C12N1/00; C12N1/22; C12Q1/04; C12Q1/34
Foreign References:
| WO2005083056A1 | 2005-09-09 |
Other References:
JPN6011063015; ＤＥＧＵＣＨＩ， Ｓ． ｅｔ ａｌ．: '"Ｐｒｅｐａｒａｔｉｏｎ ａｎｄ ｃｈａｒａｃｔｅｒｉｓａｔｉｏｎ ｏｆ ｎａｎｏｆｉｂｒｏｕｓ ｃｅｌｌｕｌｏｓｅ ｐｌａｔｅ ａｓ ａ ｎｅｗ ｓｏｌｉｄ ｓｕｐｐｏｒｔ ｆｏｒ ｍｉｃｒｏｂｉａ' ＳＯＦＴ ＭＡＴＴＥＲ Ｖｏｌ．３， Ｎｏ．９, 2007, Ｐ．１１７０-１１７５
Attorney, Agent or Firm:
Naomi Mae
Kiko Okada
Kiko Okada
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