Title:
Method for inducing differentiation of induced pluripotent stem cells
Document Type and Number:
Japanese Patent JP6363401
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for inducing differentiation of endodermal cells from stem cells efficiently and stably.SOLUTION: The invention relates to a method for inducing differentiation of endodermal cells from induced pluripotent stem cells, the method comprising preparing a cell culture substrate having a substrate, two or more isolated cell adhesion domains and cell non-adhesion domains surrounding each cell adhesion domain formed on the substrate; seeding induced pluripotent stem cells on the cell culture substrate; and culturing the seeded induced pluripotent stem cells in a culture medium. In the differentiation inducing method, the cell non-adhesion domains on the cell culture substrate are formed by immobilizing polyethylene glycol on the substrate, the cell adhesion domains are formed by oxidizing and/or degrading at least one part of polyethylene glycol immobilized on the substrate, and the area of each cell adhesion domain is less than 0.785 mm.
Inventors:
Takuya Okazaki
Katsunori Tsuchiya
Yuji Shimizu
Akihiro Umezawa
Hidenori Akutsu
Katsunori Tsuchiya
Yuji Shimizu
Akihiro Umezawa
Hidenori Akutsu
Application Number:
JP2014114190A
Publication Date:
July 25, 2018
Filing Date:
June 02, 2014
Export Citation:
Assignee:
Dai Nippon Printing Co.,Ltd.
National Center for Child Health and Development
National Center for Child Health and Development
International Classes:
C12N5/10; C12M3/00; C12N5/074
Domestic Patent References:
| JP2012120443A | ||||
| JP2013099272A | ||||
| JP2012016292A | ||||
| JP2014236716A |
Foreign References:
| WO2009072590A1 |
Other References:
Xuan Wang, et al.,Effect of RGD nanospacing on differentiation of stem cells.,Biomaterials,2013年,Vol.34,p.2865-2874
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Hidetoshi Hanai
Satoshi Fujita
Hidetoshi Hanai