Title:
Information propagation systems and methods using modified nucleic acids
Document Type and Number:
Japanese Patent JP6363984
Kind Code:
B2
Abstract:
A method for improving a nucleic acid-based molecular computing system includes (A) identifying a computing system comprised of (i) a nucleic acid structure that includes an incompletely base-paired duplex domain, (ii) at least one polynucleotide displacement molecule that can bind with the nucleic acid structure under hybridizing conditions, such that the nucleic acid structure undergoes a transition in energy state due to a branch migration reaction involving the duplex domain, and (iii) a clashing polynucleotide molecule that competes with the polynucleotide displacement molecule for binding the nucleic acid structure under the hybridizing conditions but that cannot produce a branch migration reaction involving the duplex domain; then (B) reconfiguring at least one of the displacement molecule and the nucleic acid structure, respectively, to incorporate a chemical modification relative to a first reference molecule that comprises natural nucleosides and has the same sequence content as the displacement molecule or the nucleic acid structure, as the case may be, wherein the modification causes binding of the displacement molecule and the nucleic acid structure to have a hybridization free energy, differing from that of a first reference binding between the displacement molecule or the nucleic acid structure and the first reference molecule, such that the branch migration reaction is facilitated relative to the first reference binding; and/or (C) reconfiguring at least one of the clashing molecule and the nucleic acid structure, respectively, to incorporate a chemical modification relative to a second reference molecule that comprises natural nucleosides and has the same sequence content as the clashing molecule or the nucleic acid structure, as the case may be. The modification effected via such reconfiguring causes binding of the clashing molecule and the nucleic acid structure to have a hybridization free energy, differing from that of a second reference binding between the clashing molecule or the nucleic acid structure and the second reference molecule, such that binding of the clashing molecule is impeded relative to the second reference binding.
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Inventors:
Kleinbaum, Daniel, Jay.
Application Number:
JP2015230611A
Publication Date:
July 25, 2018
Filing Date:
November 26, 2015
Export Citation:
Assignee:
Emerald Therapeutics, Incorporated
International Classes:
G16B30/00
Domestic Patent References:
| JP2009504782A | ||||
| JP2009527248A |
Foreign References:
| WO2008097929A2 |
Other References:
Bichismita Sahu et al,Synthesis of Conformationally Preorganized and Cell-Permeable Guanidine-Based γ-Peptide Nucleic Aci,J. Org. Chem.,2010年 2月20日,Vol.74 No.4,pp.1-18
David Yu Zhang et al,Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA,Science,2009年12月18日,Vol.318,pp.1121-1125
David Yu Zhang et al,Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA,Science,2009年12月18日,Vol.318,pp.1121-1125
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Naoko Kikuta
Satoshi Fujita
Natsuo Tanaka
Naoko Kikuta