Systems and methods for model-based qPCR - Life Technologies Corporation

Title:

Systems and methods for model-based qPCR

Document Type and Number:

Japanese Patent JP6363584

Kind Code:

B2

Abstract:

A method for determining a cycle threshold for a PCR amplification curve is provided. The method includes receiving a data set for a plurality of biological samples for a PCR amplification reaction. The data set includes a plurality of amplification curves, each amplification curve associated with a biological sample of the plurality of biological samples. The method further includes performing a nonlinear optimization comprising a fit of each amplification curve to a complementary modeled amplification curve to determine a best-fit set of parameters for a modeled efficiency curve and associated amplification curve. The modeled amplification curve is based on a modeled efficiency curve. The method includes determining a cycle threshold value for each biological sample based on a complementary relationship of the modeled efficiency curve to the modeled amplification curve. In an embodiment, the nonlinear optimization is a constrained nonlinear optimization.

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Inventors:

George Wallace Earl.

Application Number:

JP2015231320A

Publication Date:

July 25, 2018

Filing Date:

November 27, 2015

Export Citation:

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Assignee:

Life Technologies Corporation

International Classes:

G16B25/20; G16B40/10

Domestic Patent References:

JP2007095070A
JP2007128483A
JP2001314194A
JP2007236219A
JP2008521104A
JP2005516630A

Foreign References:

US20100070190

Other References:

Robert G Rutledge et al,A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR,BMC Biotechnology,2008年 5月 8日,Vol.8 No.47,pp.1-28

Attorney, Agent or Firm:

Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto

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