Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
可逆的な性質を示す温度応答性シートとそれを用いた細胞シートの製造方法
Document Type and Number:
Japanese Patent JP5870408
Kind Code:
B2
Abstract:
A temperature responsive sheet is disclosed that comprises a chemically modified water-soluble elastin obtained by N-acylating at least some of the primary amines and secondary amines contained in a high molecular weight water-soluble elastin molecule and coupling some or all of carboxyl groups contained in the molecule with a glycine alkyl ester. Also is disclosed a process for producing a cell sheet that comprises preparing above the temperature responsive sheet a film that functions as a scaffold for animal cells, culturing specific cells on the film so as to prepare a cell sheet, and subsequently separating the cell sheet and the temperature responsive sheet comprising the chemically modified water-soluble elastin under conditions of no greater than the culturing temperature for the cells.

Inventors:
Koji Okamoto
Yoshino Erika
Application Number:
JP2012519427A
Publication Date:
March 01, 2016
Filing Date:
June 09, 2011
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
Kyushu Institute of Technology
International Classes:
C12M1/00; A61L27/00; C07K14/78; C07K17/02; C12M3/00; C12N5/071
Foreign References:
WO2006046626A12006-05-04
WO2006046626A12006-05-04
Other References:
JPN6011034316; MIE, M., et Al.: 'Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein' Journal of Biomedical Materials Research. Part B. Applied Biomaterials Vol.86, No.1, 2007, p.283-290
JPN6011034318; 水島康徳 et al.: '高性能細胞シート構築のための人工タンパク質材料の開発' 日本化学会第86春季年会講演予稿集II , 2006, p.899 4G4-11
JPN6011034320; 前川陽祐 et al.: '化学修飾水溶性エラスチン及びI型コラーゲン共存状態における自己集合特性' 北九州医工学術者協会誌 Vol.20, 201004, p.25-28
JPN6011034316; MIE, M., et Al.: 'Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein' Journal of Biomedical Materials Research. Part B. Applied Biomaterials Vol.86, No.1, 2007, p.283-290
JPN6011034318; 水島康徳 et al.: '高性能細胞シート構築のための人工タンパク質材料の開発' 日本化学会第86春季年会講演予稿集II , 2006, p.899 4G4-11
JPN6011034320; 前川陽祐 et al.: '化学修飾水溶性エラスチン及びI型コラーゲン共存状態における自己集合特性' 北九州医工学術者協会誌 Vol.20, 201004, p.25-28
Attorney, Agent or Firm:
Yasuhiro Noguchi