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Title:
YEAST STRAINS FOR PROTEIN PRODUCTION
Document Type and Number:
Japanese Patent JP2016165300
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods for an expression system useful for production of recombinant glycoprotein, the expression system being based on ade1 and ade2 auxotrophic strains of yeast and fungi including Pichia pastoris.SOLUTION: The invention relates to an expression system comprising: (a) a host cell in which the endogenous gene encoding an auxotrophic selectable marker protein has been removed from the genome of the host cell; and (b) an integration vector comprising: (1) a nucleic acid having an open reading frame (ORF) and being functionally linked to a weak promoter, an attenuated endogenous or heterologous promoter, a cryptic promoter, a truncated endogenous or heterologous promoter, or no promoter; (2) a nucleic acid having an insertion site for the insertion of one or more expression cassettes comprising a nucleic acid encoding peptides, proteins, and/or functional nucleic acids of interest, and (3) a targeting nucleic acid that directs insertion of the integration vector into a particular location of the genome of the host cell by homologous recombination.SELECTED DRAWING: Figure 1

Inventors:
NETT JUERGEN
Application Number:
JP2016088660A
Publication Date:
September 15, 2016
Filing Date:
April 27, 2016
Export Citation:
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Assignee:
GLYCOFI INC
International Classes:
C12N15/09; C07K14/39; C12N1/19; C12P21/02
Domestic Patent References:
JP2002505587A2002-02-19
JP2007503837A2007-03-01
Foreign References:
WO2001090393A12001-11-29
WO2001088143A12001-11-22
WO2007136865A22007-11-29
Attorney, Agent or Firm:
Makoto Ono
Kenkyo Kanayama
Mitsuaki Tsubokura
Kazuki Shigemori
Kenji Ando
Yoshikazu Iwase
Yasufumi Shiroyama