FIELD OF THE INVENTION The present invention relates to novel l-(2-chloroethyl) 3-acyl-3-alkyltriazenes and a method of synthesizing them.

TECHNOLOGY REVIEW

Substituted triazenes are potentially useful in the treatment of tumors because they are capable of alkylating DNA. For example, l-aryl-3,3-dialkyltriazenes are known. One of these, 5-(dimethγltriazeno)imidazole-4-carboxamide (DTIC) is used clinically in the treatment of metastatic melanoma and some soft tissue sarcomas. l,3-Dimethγl-3-acyltriazenes are also known. They may be synthesized by reacting methylazide with methyllithium, treating the resultant 1,3-dimethyltriazene (DMT) with potassium hydride to convert the DMT to an anion and reacting this anion with an acyl halide (Smith et al., J. Org. Chem 1986, 51, 3751).

Compounds such as dimethyltriazene and trimethyltriazene are too reactive for drug use. Substitution of an acyl group

for a methyl at the 3- position to produce a l,3-dimethyl-3- acyltriazene decreases the tendency of the triazene to hydrolyze, while retaining the ability to methylate DNA.

One disadvantage of using purely methylating agents to treat tumors is that while such agents may be effective they may also be carcinogenic themselves and so may cause addi¬ tional tumors.

SUMMARY OF THE INVENTION The present invention provides a series of l-(2-chloro- ethyl)-3-acyl-3-alkyltriazenes of the formula

C1CH ₂ CH ₂ -N=N-N

*2 wherein R _] _ is an acyl group as defined below, and R ₂ is a carbon chain radical or substituted carbon chain radical as discussed below. The invention also provides a method of synthesis for these triazenes.

It is an object of the present invention to provide a new class of 3-acyltriazenes. It is also an object of the present invention to provide a new class of 3-acyltriazenes which are useful as antitumor agents. It is also an object of the present invention to provide a new class of 3-acyl- triazenes which have decreased carcinogenicity compared to dimethylacyltriazenes. It is a further object of the present invention to provide a new class of chloroethylating agents which can be used in a variety of chemical environments, including in vivo environments as well as in vitro environ¬ ments as a source of the chloroethyl group.

One advantage of the compounds of the present invention over the l,3-dimethyl-3-acyltriazenes mentioned above is the substitution of a chlorethyl group for a methyl group at the 1- position. The reactive moiety derived from the compounds of the present invention is the 2-chloroethyl diazonium ion, a DNA cross-linking agent, which has been implicated as the

cytotoxic agent from BCNU (N,N-bis(2-chloroethyl)-N- nitrosourea) and similar drugs.

The substitution of chloroethyl for methyl at the 1- position results in a molecule which is more stable than corresponding 1,3-dimethyl-3-acyltriazenes and thus provides an additional advantage.

The compounds of the present invention are also more stable in solution than known chloroethylaryltriazenes, and therefore more suitable for clinical use. Chloroethylaryl- triazenes generally have a half-life on the order of seconds in solution at physiological pH and temperature, while the compounds of the present invention under the same conditions have half-lines on the order of hours or days.

It is expected that .in vivo, enzymatic deacylation of the l-(2-chloroethyl)-3-methyl-3-acyltriazene occurs, leading to the formation of l-(2-chloroethyl)-3-methyltriazene. This compound is expected to act both as a methylating agent and a chloroethylating cross-linking agent for DNA. The compounds of the present invention therefore exhibit another advantage in that the repair enzyme 0 ⁶ -alkylguanine-DNA alkyltrans- ferase prefers to react with methyl groups on the O^ position of guanine. Since the enzyme is destroyed during that reaction, this allows the 2-chloroethyl-modified DNA to undergo cytoxic cross-linking more efficiently than is the case for BCNU and similar drugs. The chloroethylacyltriazenes of the present invention are therefore expected to be strongly cytotoxic. The compounds of the present invention are also expected to be less toxic to bone marrow (less myleosuppres- sive) than BCNU. Additionally, BCNU on hydrolysis produces significant amounts of acetaldehyde in an alternate reaction path which decreases the amount of chloroethyl diazonium ion available for DNA crosslinking. This has also been observed for chloroethylaryltriazenes. In contrast, compounds of the present invention produce little or no acetaldehyde on

hydrolysis and therefore have another advantage over the above-mentioned compounds.

Compounds of the present invention..are also useful in chemical synthesis as chloroethylating agents, since sol- volysis (for example, in water) at neutral pH leads to production of the chloroethyl diazonium ion.

BRIEF DESCRIPTION OF THE DRAWING Scheme 1 shows the general method of synthesis. 2- Azidoethanol, (1), is treated with tert-butylchlorodimethyl- silane to produce l-azido-2-(tert-butyldimethylsiloxy)ethane (2) . This is then treated with methyllithium (or other organometallic) to produce the corresponding 3-alkylated triazene (3) . This compound is then acylated (4) either by treatment with an isocyanate or by treatment with KH to form an anion followed by reaction with an acyl halide, acid anhydride or similar acylating agents. The hydroxy group is then deprotected by treatment with tetra-n-butylammonium fluoride to produce the 2-hydroxyethyl triazene (5), and the chloride is then produced by reaction with Ph3P/CCl (6).

DESCRIPTION OF THE PREFERRED EMBODIMENTS

The acyl groups as defined in this application include: carbonyl with an alkyl substituent (1-oxoalkyl) containing from one to seven carbon atoms; or benzoyl; or phenylacetyl; carboalkoxy groups containing from two to eight carbon atoms, and fluorinated derivatives of these each containing up to three fluorine atoms; carbophenoxy; N-phenyl and N-methyl substituted aminocarbonyls; thiocarbamoyl; N-substituted thiocarbamoyls in which the substituent is an alkyl group with up to seven carbon atoms, or a phenyl group; dialkyl- phosphonates in which the alkyl groups can be the same or different and each contains from one to seven carbon atoms; and phenylsulfonyl or ^' meta or para-substituted phenylsul¬ fonyl, in which the meta or para substituent is a methyl or ethyl group, a methoxy or ethoxy group, or a halogen.

As can be seen from the above, a wide variety of acyl groups can be attached to the triazene skeleton to control the stability or decomposition of the triazene under specific conditions such as occur in vivo. Another method of con- trolling the reactivity of the triazenes of the invention is by varying the alkyl group at the 3- position. An electron- withdrawing substituent such as 3, 3, 3- trifluoropropyl, benzyl or the like, favors a decomposition pathway resulting in production of the chloroethyl diazoniu ion (rather than an alkyldiazonium ion) and therefore a higher degree of DNA cross-linking.

The substituent groups at the 3-position encompassed by this invention include: alkyl containing from one to seven carbon atoms, or such alkyl groups substituted with from one to three fluorine atoms wherein the α carbon of the alkyl group is not substituted with fluorine; benzyl or meta or para-substituted benzyl in which the meta or para substituent is chlorine or fluorine, or an alkoxy group with from one to four carbon atoms; and alkenyl or alkynyl groups containing from two to seven carbon atoms.

The following example will illustrate a method for syn¬ thesizing these compounds. Example

Safety Note. Triazenes are potent biological alkylating agents and as such should be considered to be toxic and potentially carcinogenic compounds. Efficient hoods and protective clothing should be used at all times in working with these substances. Alkyl azides are potentially ex¬ plosively unstable and suitable precautions ought to be observed in the handling of these substances.

Materials. All chemicals were reagent grade (Aldrich), used as purchased without further purification. IR and UV spectra were obtained on a Perkin-Elmer Model 297 infrared spectrophotometer and a Hewlett-Packard Model 8450A double beam diode array processor, respectively. NMR spectra were obtained on a Varian XL-200 spectrometer. Exact mass measure-

ments were determined on either a VG-Micromass, ZAB-2F (for FAB Spectra) or a VG 70-250 (for El spectra) mass spectrometer. Mass measurements were confirmed by peak matching. 2-Azidoethanol, JL. 2-Chloroethanol (241.5g, 3.0 mol) was added rapidly to a solution of sodium azide (234.5g, 3.61 mol) in 800 mL of water at room temperature. The reaction mixture was stirred at 30°C for 1 h and then at 70°C for 24 h. The resulting red solution was cooled to room tempera- ture, saturated with sodium sulfate, and extracted with 4x400 mL of methylene chloride. The combined organic layers were dried two times over anhydrous sodium sulfate and concentrated on a rotary evaporator at 25°C to give 206g (2.37 mol, 79.0%) of crude 2-azidoethanol. Although this substance can be purified by reduced pressure distillation (77°C, 24 mm), it was found that the above obtained material was of adequate purity to be used directly in the next reaction. This was also desirable given the highly explosive nature of low molecular weight organic azides. l-Azido-2-(tert-butyldimethylsiloxy)ethane, 2_. 2-

Azidoethanol, 1_, (105g, 1.21 mol), crude from above prepara¬ tion, and tert-butylchlorodimethylsilane (200 g. 1.33 mol) were dissolved in 200 mL of dry N,N-dimethylformamide (DMF) and stirred under nitrogen. Over a period of 20 min, im- idazole (204 g, 3.00 mol) was added portionwise with stirring and the temperature of the reaction rose to about 40°C. The solution was stirred at 40°C under nitrogen for 24 h. The reaction mixture was cooled to room temperature, poured into 1 L of water, and extracted with pentane (4x300 mL) . The pentane layers were dried over anhydrous sodium sulfate, concentrated on a rotary evaporator at 25°C, and distilled at reduced pressure through a 4 in. Vigreux column to give 230 g (1.14 mol, 94.7%) of l-azido-2-(tert-butyldimethyl¬ siloxy)ethane, 2 : bp 37-39°C (0.02 mm); IR (CH ₂ C1 ₂ ) 2940, 2860, 2120, 1115, 940, 840 cm ^"1 , NMR (CD1 ₃ , Me4Si) δ 0.09 (6H,s), 0.92 (9H,s), 3.27 (2H,t,J = 5 Hz), 3.80 (2H,t,J =

5Hz); proton decoupled ¹³ C NMR (CDC1 ₃ , Me Si)) δ -5.54, 18.18, 25.77, 53.16, 62.59.

1-(2-(tert-Butyldimethylsiloxy)ethyl)-3-methyltriazene, 3_. A 1.4 M ether solution of methyllithium (468 mL. 0.656 mol) was added dropwise over a period of 1.5 h to a stirred solution of l-azido-2-(tertbutyldimethylsiloxy)ethane, 2 , in anhydrous ether (200 mL) at -20°C under nitrogen. [On occasion, the reaction mixture turned to a gel during the addition, necessitating the addition of sufficient anhydrous ether and mechanical agitation to disrupt the gel before completing addition of the methyllithium solution] . The resulting yellow solution was allowed to warm gradually to room temperature over 2 h. It was then cooled to 0°C and hydrolyzed by careful addition of 100 mL of a 10% ammonium hydroxide solution containing 10% (w/v) ammonium chloride. The ether layer was separated and the aqueous layer was reextracted with pentane (300 mL) . The ether and pentane layers were combined, dried over anhydrous sodium sulfate, and concentrated on a rotary evaporator at 25°C. Distilla- tion at reduced pressure through a 4 in Vigreux column was then replaced with a short path distillation head and the distillation continued, yielding 54.2 g (0.419 mol, 70.4%) of l-(2-(tert-butyldimethylsiloxy)ethyl)-3-methyltriazene, 3_: bp 54-56°C (0.02mm); IR (CH ₂ C1 ₂ ) 3480, 2940, 2860, 1100, 840 cm ^{" 1} ;1H NMR (CDCI3, Me ₄ Si) δ 0.08 (6H,s), 0.89 (9H,s), 1.58 (lH,b), 3.23 (3H,b), 3.59 (2H,broad t,J= 5 Hz), 3.83 (2H,t,J=5 Hz); proton decoupled ¹³ C NMR (CDC1 ₃ , Me Si) δ - 5.36, 18.30, 25.86, (30.89) 46.73, 47.91 (60.98), (61.95) 62.80. 1-(2-(tert-Butyldimethylsiloxy)ethyl)-3-carboethoxy-3- methyltriazene, 4a. A solution of l-(2-(tert-butyldimethyl- siloxy)ethyl)-3-methyltriazene, 3_, (24.0 g, 0.11 mol) in anhydrous ether (70 mL) was added dropwise over 1.5 h to a stirred suspension of potassium hydride ( ~ 6g, 0.15 mol) in anhydrous ether containing 50 mg (1.3 x 10-* mol) of dicyclohexano-18-crown-6 ether at 25°C under nitrogen.

Stirring was continued for 30 min until hydrogen evolution had ceased. The reaction mixture was cooled to -30°C and then ethyl chloroformate (13.1 g, 0.12 mol) in anhydrous ether (70 mL) was added dropwise with stirring over 2 h. the reaction was stirred and allowed to warm gradually to 0°C over 30 minutes, at which time hydrolysis was accomplished by the careful addition of 250 mL of 10% ammonium hydroxide solution containing 10% (w/v) ammonium chloride. The ether layer was separated and the aqueous layer was reextracted with pentane (100 mL) . The ether and pentane layers were combined and dried twice over anhydrous sodium sulfate, and concentrated on a rotary evaporator at 25°C. Distillation of 1/10 of the crude product at reduced pressure using a short path distillation head gave 1.68 g (6.45 x 10~ ² mol, 58.6% yield based on the fraction of total product distilled) of 1- (2-(tert-butyldimethylsiloxy)ethyl)-3-carboethoxy-3-methyl- triazene, 4a.: bp 90-95°C (0.07 mm); IR(C1 ₄ ) 2960, 2940, 2860, 1725, 1160, 840 cm ^"1 ; ^X H NMR (CDCI3, Me ₄ Si) δ 0.04 (6H,s), 0,87 (9H,s), 1.37 (3H,t,J=7 Hz), 3.24 (3H,s), 3.92 (2H,m), 4.94 (2H,m), 4.38 (2H,q,J=7 Hz); proton decoupled ¹³ C NMR (CDCI3, Me ₄ Si) δ -5.36, 14.45, 18.21, 25.89, 29.41, 61.20, 62.87, 63.87, 154.5. The crude product was determined to be sufficiently pure to be used directly in the following reaction. 3-Carboethoxy-2-(2-hydroxyethyl)-3-methyltriazene, 5a. A 1.0 M tetrahydrofuran solution of tetra-n-butylammonium fluoride (140 mL, 0.14 mol) was added portionwise over 5 min with stirring to a solution of 1-(2-(tert-butyldimethyl- siloxy)ethyl)-3-carboethoxy-3-methyltriazene, 4a, (29.0 g, 0.133 mol) in tetrahydrofuran (20 mL) . The reaction solution was heated to 35°C and stirring continued for 30 min. The volume of the reaction was reduced by one half on a rotary evaporator at 25°C and the resultant solution was chromato- graphed on a column of 300 g of Silica Gel 60 (EM, neutral.

70-230 mesh) packed in 1:1 pentane-ether. The column was eluted with 4 L of ether with about 20 psi head pressure of nitrogen. The first 200 mL of eluent contained a large amount of an oil assumed to be tert-butylfluorodimethyl- silane. Remaining eluent fractions contained a mixture of three or four components with one substance (rf 0.35 on F-60 silica gel plate, EM, eluted with ether and visualized by short wavelength UV light) . These fractions were recombined (18.6 g) and rechromatographed on a column of 200 g of Silica Gel 60 packed in pentane and eluted sequentially with 500 mL each of pentane; 25%, 50%, and 75% ether in pentane, and finally with 2L of ether. The first liter of ether eluent contained the desired product, 3-carboethoxy-l-(2-hydroxy- ethyl)-3-methyltriazene, 5a, 4.58 g (0.026 mol, 19.5%). Attempts to further purify this substance by reduced pressure (0.002 mm) distillation generally failed as a result of thermal decomposition during distillation. IR (CH ₂ C1 ₂ ) 3600, 3460, 2940, 1710, 1155, 990 cm ^-1 ; UV (CH3CN) λ _maχ 233 (log ε 4.14); ^λ E NMR (CDCI3, Me ₄ Si) 61.36 (3H,t,7 Hz), 1.66 (lH,b), 3.25 (3H,s), 3.98 (4H,s), 4.37 (2H,g,J=7 Hz); proton decoupled ¹³ C NMR (CDCI3, Me ₄ Si) δ 14.48*, 29.53, 61.02, 63,14, 63.38*, 154.4* (*=attached proton test, APT, indicated odd number of protons attached) ; exact mass calcd m/z for C ₆ H ₁₄ N ₃ 0 ₃ (M+l by FAB) 176.1035, found 176.1031. 3-Carboethoxy-l-(2-chloroethyl)-3-methyltriazene, 6a. A solution of triphenylphosphine (2.83 g, 1.08 X 10~ ² mol) and 3-carboethoxy-l-(2-hydroxyethyl)-3-methyltriazene, j5a_, (1.89 g, 1.08 x 10 ^~2 mol) in 25 mL of carbon tetrachloride (dried over 3A molecular sieves) was refluxed overnight under nitrogen. The reaction mixture was cooled to room tempera¬ ture and diluted with pentane (100 mL) . After standing at 10°C for four hours, the reaction mixture was filtered through a pad of Celite to remove precipitated triphenyl¬ phosphine oxide. The filtrate was concentrated on a rotary

evaporator at 22°C, redissolved in a minimum amount of ether and chromatographed on a column of 40 g of Silica Gel 60 packed in 1:10 ether-pentane and eluted with 200 mL each of 10%, 20% and 30% ether in pentane. The first 200 mL of eluent contained 1.4 g of a yellow oil. Distillation of that oil at reduced pressure with a short path distillation apparatus produced 1.22 g (6:30 x 10~ ³ mol, 58.3%) of the desired compound, 3-carboethoxy-(2-chloroethyl)-3-methyl- triazene, 6a.:bp 60-62°C (0.003 mm); IR (CC1 ₄ ) 2990, 2970, 1730, 1155, 990 cm ^"1 ; UV (CH3CN) λ _maχ 2333 (log ε 4.19); NMR 9CDC1 ₃ , Me ₄ Si) δ 1.39 (0-CH ₂ CH_ ₃ ,t,J=7 Hz), 3.28 (N- CH ₃ ,S), 3.88 (Cl-CH ₂ ,t,J=6 Hz), 4.12 (CH ₂ -N,t,J= 6Hz) , 4.39 (0-CH ₂ CH ₃ ,q,J=7 Hz); proton decoupled ¹³ C NMR (CDCI3, Me Si) δ 14.47 (0-CH ₂ CH ₃ ), 29.76 (N-CH3), 41.92 (C1-CH ₂ ), 62.50 (CH ₂ -N), 63.17 (0-CH ₂ CH ₃ ), 154.27 (C=0); ( and ¹³ C NMR assignments made via ¹³ C-APT and 2D-heteronuclear correlated spectrum (2D-COSY) experiments); exact mass calcd m/z for C ₆ H ₁₂ N230 ₂ C1193.0618, found 193.0629 (by El).

3-Acetyl-l-(2-(tert-butyldimethylsiloxy)ethyl)-3- methyltriazene, 4b. A solution of l-(2-(tert-butyldimethyl¬ siloxy)ethyl)-3-methyltriazene, 3_, (21.74 g. 0.10 mol) in ether (50mL) was added dropwise over 1.5 h to a stirred suspension of potassium hydride (4.41 g, 0.11 mol) in an¬ hydrous ether containing 50 mg (1.3 x 10~ ⁴ mol) of dicyclo- hexano-18-crown-6 ether at room temperature under nitrogen. When hydrogen evolution had ceased, the reaction mixture was cooled to -10°C and a solution of acetyl chloride (8.64 g, 0.11 mol) in ether (40 mL) was added dropwise over 2 h. The reaction mixture was allowed to warm to 0°C over 1 h and hydrolyzed by the addition of 75 mL of 10% ammonium hydroxide containing 10% (w/v) of ammonium chloride. The ether layer was separated and the aqueous layer was extracted with pentane (2 x 100 mL) . The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated on a

rotary evaporator. The residue was distilled at reduced pressure through a 4 in. Vigreux distillation column to give, after a small forefraction, 15.7 g (0.0645 mol, 64.5%) of 3- acetyl-1-(2-(tertObutyldimethylsiloxy)-ethyl)-3-methyltria- zene, 4b: bp 78-84°C (0.08 mm); IR (CH ₂ C12) 2960, 2950,

2860, 1685, 1150, 1105, 980, 840 cm ^"1 ; NMR 3DC1 ₂ , Me ₄ Si) δ 0.04 (6H,s), 0.86 (9H,s), 2.43 (3H,s), 3.21 (3H,s), 3.93 (2H,t.J=5 Hz), 4.00 (2H,t,J=5 Hz); proton decoupled ¹³ C NMR (CDC1 ₃ , Me ₄ Si) δ -5.32, 18.20, 21.83, 25.75, 26.60, 61.00, 64.50, 173.2.

3-Acetyl-l-(2-hydroxyethyl)-3-methyltriazene, 5b. A 1.0 M solution (68 L) of tetra-n-butγlammonium fluoride in tetrahydrofuran was added dropwise to a stirred solution of 3-acetyl-l-(2-(tert-butyldimethyl-siloxy)ethyl)-3-methyltria - zene, 4b, in tetrahydrofuran at 0°C. The reaction solution was allowed to warm gradually to room temperature over 1 h. The volume of the solution was reduced 50% with a rotary evaporator at 25°C and the residue was chromatographed on a column of 200 g of Silica Gel 60 (EM, neutral, 70-230 mesh) packed in 3:1 ether-pentane. The column was eluted with 4 L of 4:1 ether-pentane. The last 3 L of solvent were con¬ centrated on a rotary evaporator to give 6.75 g (0.0465 mol, 72.5%) of 3-acetyl-l-(2-hydroxethyl)-3-methyltriazene, 5b, as a colorless oil, which fails to distill satisfactorily at reduced pressure (0.002 mm). IR (CH ₂ C1 ₂ ) 3600, 3470, 2940, 1680, 1140, 975 cm ^"1 ; UV (CH3CN) λ _maχ 237 (log ε 4.09); ^X H NMR (CDCI3, Me Si) δ 2.09 (lH,b), 2÷44 (3H,s), 3.22 (3H,s), 3.96 (2H,m), 4.04 (2H,m); proton decoupled ¹³ C NMR (CDC1 ₃ , Me ₄ Si)δ 21.83 (CH ₃ ) , 26.80 (CH ₃ ) , 60.85 (CH ₂ ) , 64.00 (CH ₂ ) , 173.15 (C=0) [APT experiment allowed assignment of the number of attached protons]; exact mass calcd. m/z for C5H11N3O; _? 145.0851, found 145.0848 (by El).

3-Acetyl-l-(2-chlorethyl)-3-methyltriazene, 6b. A solution of triphenylphosphine (5.89 g, 2.25 x 10" ² mol) and

3-carboethoxy-l-(2hydroxyethyl)-3-methyltriazene, 5b, (2.9 g, 2.0 x 10~ ² mol) in 50 mL of carbon tetrachloride (dried over 3A molecular sieves) was refluxed overnight under nitrogen. The reaction mixture was cooled to room temperature and diluted with pentane (250 mL) . After standing at 10°C overnight, the reaction mixture was filtered through a pad of Celite to remove precipitated triphenylphosphine oxide. The filtrate was concentrated on a rotary evaporator at 220°C, the residue (4.2 g) was dissolved in a minimum amount of ether and chromatographed on a column of 35 g of Silica Gel 60 packed in 10% ether in pentane and eluted with 600 mL of this same solvent mixture. Evaporation of the eluent on a rotary evaporator and vacuum transfer (40°C/0.005 mm) of the residue gave 1.94 g (0.0119 mol, 59.6%) of 3-acetyl-l-(2- chloroethyl)-3-methyltriazene, 6b; IR (CC1 ₄ ) 2970, 1710,

1515, 1145, 990 cm" ¹ ; UV (CH3CN) λ_. _maχ 238 (log ε 4.23); !H NMR (CDCI3, Me ₄ Si) δ 3.45 (CH ₃ ,s), 3.23 (N-CH ₃ ,s), 3.90 (Cl- CH ₂ ,t,J=5.5 Hz), 4.12 (CH ₂ -N,t,J=5.5 Hz); proton decoupled ¹³ C NMR (CDCI3, Me ₄ Si) δ 21.85 (CH3( , 26.92 (N-CH3), 41.78 (C1-CH2), 62.68 (CH ₂ -N) , 63.17 (0-CH ₂ CH ₃ ), 173.0 (C=0); exact mass calcd. m/z for C5H ₁₀ N ₃ 0C1 163.0512, found 163.0506 (by El).

1-(2-(tert-Butyldimethylsiloxy)ethyl)-3-(N-methylcar- bamoyl)-triazene, 4c. Methyl isocyanate (5.99 g. 0.105 mol) was added dropwise to a stirred solution of l-(2-(tert- butyldimethylsiloxy)ethyl)-3-methyltriazene, 3_, (21.74 g, 0.10 mol) in pentane (75 mL) , under nitrogen, and at such a rate to maintain a gentle reflux. Following this addition, the reaction mixture was stirred at 30°C for 30 min and then concentrated on a rotary evaporator at 25°C to yield 27.17 g of 1-(2-(tert-butyldimethylsiloxy)ethyl)-3-methy1-3-(N- methylcarbamoyl)triazene as a colorless oil. 'H NMR (CDCI3, Me ₄ Si) δ 0.01 (6H, s), 0.84 (9H,s), 2.92 (3H,d,J=5Hz) , 3.22 (3H,s), 3.82 (2H,t,J=5Hz) , 3.97 (2H,t,J-5Hz) , 6.35 (lH,b) ;

proton decoupled ¹³ C NMR (CDC1 ₃ , Me ₄ Si) δ - 5.35, 18.25, 25.79, 26.86, 27,53, 61.12, 155.64.

1-(2-Hydroxyethyl)-3-methyl-3-(N-methylcarbamoyl)tri¬ azene, 5c. A 1.0 M solution (100 mL) of tetra-n-butylam- monium fluoride in tetrahydrofuran was added dropwise to a stirred solution of l-(2-(tert-butyldimethylsiloxy)ethyl)-3- methyl-3-(N-methγlcarbamoyl)triazene in tetra-hydrofuran at - 10°C over 1 h. The volume of the solution was then reduced 50% with a rotary evaporator at 25°C and the resultant solution was chromatographed on a column of 250 g of Silica Gel 60 (EM, neutral, 70-230 mesh) packed in ether. The column was eluted with 5 L of ether. The last 3L of eluent were concentrated on a rotary evaporator and the residue (6.7 g) recrystallized from methylene chloride-ether to give 4.24 g of l-(2-hydroxyethyl)-3-methyl-3-(N-methylcarbamoyl)tri¬ azene, 5 , (0.0265 mol, 27.8%): p 72-74°C; IR (CH ₂ C1 ₂ ) 3600, 3450, 2955, 1690, 1510, 1190, 1050 cm ^"1 ; UV (CH3CN) λ _raaχ 245 (log ε 4.04); NMR (CDCI3, Me ₄ Si) δ 1.78 (1H,t,J=6Hz) , 2.94 (3H,d,J=5 Hz), 3.26 (3H,s), 3.97 (2H,t,J=5 Hz), 4.02 (2H,m- A ₂ B ₂ X), 6.34 (lH,b); proton decoupled ¹³ C NMR (CDCI3, Me ₄ Si) δ 26.86, 27.60, 60.72, 155.54; exact mass calcd m/z for C ₅ H ₁₂ N ₄ o ₂ 160.0960, found 160.0967 (by El). l-(2-Chloroethyl)-3-methyl-3-(N-methylcarbamoyl)tri¬ azene, 6c. A solution of triphenylphosphine (6.22 g, 2.37x10" ² mol) and 3-σarboethoxy-l-(2-hydroxyethyl)3-methyltriazene, 5c, (3.8 g, 2.37 x 10~ ² mol) in 60 mL of carbon tetrachloride (dried over 3A molecular sieves) was refluxed overnight under nitrogen. The reaction mixture was cooled to room temperature and diluted with pentane (220 mL) . After standing at 10°C overnight, the reaction mixture was filtered through a pad of Celite to remove precipitated tiiphenylphosphine oxide. The filtrate was concentrated on a rotary evaporator at 22°C, redissolved in a minimum amount of ether and chromatographed on a column of 35 g of Silica Gel 60 packed in 10% ether in

pentane and eluted with 350 mL each of 10, 20, 30, 40 and 50 ^l % ^! ether in pentane. Concentration of the last 1 L of eluent on a rotary evaporator and recrystallization of the residue from ether-pentane gave 2.38 g of l-(2-chloroethyl)-3-methyul-3-(N- methylcarbamoyl)triazene, €ϊc, (0.0133 mol, 56.2%) :mp 48.5- 49.5°C; IR (CC1 ₄ ) 3460, 3400 (weak), 2970, 2910, 1705, 1500, 1190, 1070, 1050 cm ^"1 ; UV (CH3CN) λ _maχ 245.5 (log ε 4.05); ^X H NMR (CDCI3 (CDCI3, Me ₄ Si) δ 2.95 (NH-CH3,d,J=5 Hz), 3.26 (N- CH ₃ ,s), 3.86 (CH ₂ -Cl,t,J=6 Hz), 4.05 (CH ₂ -N,t,J=6 Hz), 6.37 (N-H,b); proton decoupled ¹³ C NMR ((CDCI3, Me Si) δ 26.87 (NH- CH3), 27.70 (N-CH3), 41.97 (CH ₂ -C1), 62.43 (CH ₂ -N) , 155.2 (C=0); { ^-B. and ¹³ H NMR assignments made via ¹³ C-APT and 2D- COSY spectra); exact mass calcd m/z for C5H 1N4OCI 178.0621, found 178.0598 (by El). It will be understood that the above description of the present invention is susceptible to various modifications, changes and ^' adaptations, and the same are intended to be comprehended within the meaning and range of equivalents of the appended claims.