Title:
18F LABELING OF PROTEINS USING SORTASES
Document Type and Number:
WIPO Patent Application WO/2015/073746
Kind Code:
A3
Abstract:
The present invention, in some aspects, provides methods, reagents, compositions, and kits for the radiolabeling of proteins, for example, of proteins useful for positron emission tomography (PET) or single-photon emission computed tomography (SPECT) (e.g., for diagnostic and therapeutic applications), using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a radioactive agent or molecule to diagnostic or therapeutic peptides or proteins. Compositions comprising sortagged, radiolabeled proteins as well as reagents for generating radiolalebed proteins are also provided. Kits comprising reagents useful for the generation of radiolabeled proteins are provided, as are precursor proteins that comprise a sortase recognition motif.
Inventors:
RASHIDIAN MOHAMMAD (US)
PLOEGH HIDDE L (US)
PLOEGH HIDDE L (US)
Application Number:
PCT/US2014/065574
Publication Date:
October 29, 2015
Filing Date:
November 13, 2014
Export Citation:
Assignee:
WHITEHEAD BIOMEDICAL INST (US)
International Classes:
C12N9/50; C07K16/00; C12P21/06
Domestic Patent References:
| WO2013003555A1 | 2013-01-03 |
Other References:
"Wu. F-18 Labeled Diabody-Luciferase Fusion Proteins for Optical-ImmunoPET.", 18 January 2013 (2013-01-18), Retrieved from the Internet [retrieved on 20150223]
COOPER ET AL.: "Comparison of (64)Cu-complexing bifunctional chelators for radioimmunoconjugation: labeling efficiency, specific activity, and in vitro/in vivo stability.", BIOCONJUG CHEM., vol. 23, no. 5, 2012, pages 1029 - 39, XP055187002, DOI: doi:10.1021/bc300037w
NAMAVARI ET AL.: "A novel method for direct site-specific radiolabeling of peptides using [18F]FDG.", BIOCONJUG CHEM., vol. 20, no. 3, 2009, pages 432 - 436, XP055035206
PATERSON ET AL.: "Enzyme-mediated site-specific bioconjugation of metal complexes to proteins: sortase-mediated coupling of copper-64 to a single-chain antibody.", ANGEW CHEM INT ED ENGL., vol. 53, no. 24, pages 6115 - 9
COOPER ET AL.: "Comparison of (64)Cu-complexing bifunctional chelators for radioimmunoconjugation: labeling efficiency, specific activity, and in vitro/in vivo stability.", BIOCONJUG CHEM., vol. 23, no. 5, 2012, pages 1029 - 39, XP055187002, DOI: doi:10.1021/bc300037w
NAMAVARI ET AL.: "A novel method for direct site-specific radiolabeling of peptides using [18F]FDG.", BIOCONJUG CHEM., vol. 20, no. 3, 2009, pages 432 - 436, XP055035206
PATERSON ET AL.: "Enzyme-mediated site-specific bioconjugation of metal complexes to proteins: sortase-mediated coupling of copper-64 to a single-chain antibody.", ANGEW CHEM INT ED ENGL., vol. 53, no. 24, pages 6115 - 9
Attorney, Agent or Firm:
BAKER, C., Hunter (Greenfield & Sacks P.C.,600 Atlantic Avenu, Boston MA, US)
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