Title:
2-ISOPROPYLMALATE SYNTHASE, ENGINEERED BACTERIA OF SAME, AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/037190
Kind Code:
A1
Abstract:
Provided are a 2-isopropylmalate synthase, L-leucine-producing genetically engineered bacteria, and applications thereof, related to the field of metabolic engineering. The genetically engineered bacteria are acquired by overexpressing in a host cell L-leucine feedback inhibition-releasing isopropylmalate synthase coding gene leuAM, L-isoleucine feedback inhibition-releasing acetolactate synthase coding gene ilvBNM, 3-isopropylmalate dehydrogenase coding gene leuB, and 3-isopropylmalate dehydratase coding gene leuCD. The L-leucine genetically engineered bacteria are free of auxotrophy, grow rapidly, and have a short fermentation cycle, high yield, and high conversion rate.
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Inventors:
ZHANG CHENGLIN (CN)
XU QINGYANG (CN)
LI YANJUN (CN)
ZHANG YU (CN)
LI YINGZI (CN)
ZHU FUZHOU (CN)
LU NAN (CN)
HAN SHIBAO (CN)
DONG XIERONG (CN)
WANG ZISHEN (CN)
XU HAO (CN)
LI ZIYI (CN)
XU QINGYANG (CN)
LI YANJUN (CN)
ZHANG YU (CN)
LI YINGZI (CN)
ZHU FUZHOU (CN)
LU NAN (CN)
HAN SHIBAO (CN)
DONG XIERONG (CN)
WANG ZISHEN (CN)
XU HAO (CN)
LI ZIYI (CN)
Application Number:
PCT/CN2020/112038
Publication Date:
March 04, 2021
Filing Date:
August 28, 2020
Export Citation:
Assignee:
TIANJIN UNIV OF SCIENCE AND TECHNOLOGY (CN)
International Classes:
C12N9/10; C12N15/52; C12N15/77; C12P13/06
Foreign References:
| CN110540976A | 2019-12-06 | |||
| CN110551670A | 2019-12-10 | |||
| CN104480058A | 2015-04-01 | |||
| CN105492616A | 2016-04-13 | |||
| CN108884449A | 2018-11-23 |
Other References:
WANG YING-YU, ZHANG FENG, XU JIAN-ZHONG, ZHANG WEI-GUO, CHEN XIU-LAI, LIU LI-MING: "Improvement of l-Leucine Production in Corynebacterium glutamicum by Altering the Redox Flux", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 8, pages 2020, XP055786913, DOI: 10.3390/ijms20082020
WANG YING-YU, XU JIAN-ZHONG, ZHANG WEI-GUO: "Metabolic engineering of l-leucine production in Escherichia coli and Corynebacterium glutamicum: A Review.", CRITICAL REVIEWS IN BIOTECHNOLOGY, CRC PRESS, BOCA RATON, FL, US, vol. 39, no. 5, 4 July 2019 (2019-07-04), US, pages 633 - 647, XP055786915, ISSN: 0738-8551, DOI: 10.1080/07388551.2019.1577214
WANG YING-YU, XU JIAN-ZHONG, ZHANG WEI-GUO: "Metabolic engineering of l-leucine production in Escherichia coli and Corynebacterium glutamicum: A Review.", CRITICAL REVIEWS IN BIOTECHNOLOGY, CRC PRESS, BOCA RATON, FL, US, vol. 39, no. 5, 4 July 2019 (2019-07-04), US, pages 633 - 647, XP055786915, ISSN: 0738-8551, DOI: 10.1080/07388551.2019.1577214
Attorney, Agent or Firm:
BEIJING RISINGMARK INTELLECTUAL PROPERTY AGENCY (CN)
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