Title:
2C-LIKE TOTIPOTENT STEM CELLS INDUCED BY MATERNAL FACTORS, AND TRANSFORMATION USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/272775
Kind Code:
A1
Abstract:
Provided are a 2C-like totipotent stem cell induced by maternal factors, and the transformation use thereof, wherein the 2C-like totipotent stem cell is generated using four maternal factors, i.e. Hsf1, Zar1, Padi6 and Npm2; and methods related thereto are provided. Furthermore, a method for promoting the in-vitro differentiation of the 2C-like totipotent stem cells into blastocysts and zona pellucida blastocysts is provided; and it is proved that the four maternal factors can reprogram somatic cells into 2C-like totipotent stem cells.
Inventors:
GUI LIMING (CN)
WU RUIFANG (CN)
WU RUIFANG (CN)
Application Number:
PCT/CN2021/105684
Publication Date:
January 05, 2023
Filing Date:
July 12, 2021
Export Citation:
Assignee:
UNIV PEKING SHENZHEN HOSPITAL (CN)
International Classes:
C12N5/0789; C07K14/475; C12N5/073; C12N15/09; C12N15/63
Domestic Patent References:
| WO2018204764A1 | 2018-11-08 |
Foreign References:
| US20210180026A1 | 2021-06-17 | |||
| CN108884436A | 2018-11-23 |
Other References:
HU ZHENHUA; TAN DENNIS ENG KIAT; CHIA GLORYN; TAN HAIHAN; LEONG HWEI FEN; CHEN BENJAMIN JIEMING; LAU MEI SHENG; TAN KELLY YU SING;: "Maternal factor NELFA drives a 2C-like state in mouse embryonic stem cells", NATURE CELL BIOLOGY, NATURE PUBLISHING GROUP UK, LONDON, vol. 22, no. 2, 13 January 2020 (2020-01-13), London, pages 175 - 186, XP037010239, ISSN: 1465-7392, DOI: 10.1038/s41556-019-0453-8
KIM KYEOUNG-HWA, LEE KYUNG-AH: "Maternal effect genes: Findings and effects on mouse embryo development", CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE, vol. 41, no. 2, 1 January 2014 (2014-01-01), pages 47 - 61, XP093018734, ISSN: 2233-8233, DOI: 10.5653/cerm.2014.41.2.47
PIRAYE YURTTAS, ALEJANDRA M. VITALE, ROBERT J. FITZHENRY, LEONA COHEN-GOULD, WENZHU WU, JAN A. GOSSEN, AND SCOTT A. COONROD: "Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 135, 26 May 2008 (2008-05-26), GB , pages 2627 - 2636, XP008154132, ISSN: 0950-1991, DOI: 10.1242/dev.016329
RONG YAN, JI SHU-YAN, ZHU YE-ZHANG, WU YUN-WEN, SHEN LI, FAN HENG-YU: "ZAR1 and ZAR2 are required for oocyte meiotic maturation by regulating the maternal transcriptome and mRNA translational activation", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 47, no. 21, 2 December 2019 (2019-12-02), GB , pages 11387 - 11402, XP093018741, ISSN: 0305-1048, DOI: 10.1093/nar/gkz863
KIM KYEOUNG-HWA, LEE KYUNG-AH: "Maternal effect genes: Findings and effects on mouse embryo development", CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE, vol. 41, no. 2, 1 January 2014 (2014-01-01), pages 47 - 61, XP093018734, ISSN: 2233-8233, DOI: 10.5653/cerm.2014.41.2.47
PIRAYE YURTTAS, ALEJANDRA M. VITALE, ROBERT J. FITZHENRY, LEONA COHEN-GOULD, WENZHU WU, JAN A. GOSSEN, AND SCOTT A. COONROD: "Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 135, 26 May 2008 (2008-05-26), GB , pages 2627 - 2636, XP008154132, ISSN: 0950-1991, DOI: 10.1242/dev.016329
RONG YAN, JI SHU-YAN, ZHU YE-ZHANG, WU YUN-WEN, SHEN LI, FAN HENG-YU: "ZAR1 and ZAR2 are required for oocyte meiotic maturation by regulating the maternal transcriptome and mRNA translational activation", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 47, no. 21, 2 December 2019 (2019-12-02), GB , pages 11387 - 11402, XP093018741, ISSN: 0305-1048, DOI: 10.1093/nar/gkz863
Attorney, Agent or Firm:
SHENZHEN SEMHOPE GUANGLIANINTELLECTUAL PROPERTY AGENCY (CN)
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