4-SϋBSTITϋTED 1.2,4-TRIAZOLE DERIVATIVES

The present invention relates to a discrete class of 4-substituted 1,2,4-triazole derivatives which act on 5-hydroxytryptamine (5-HT) receptors, being selective agonists of so-called "S-HTi-like" receptors. They are therefore useful in the treatment of clinical conditions for which a selective agonist of these receptors is indicated.

5-HTι-like receptor agonists which exhibit selective vasoconstrictor activity have recently been described as being of use in the treatment of migraine (see, for example, A. Doenicke et al.. The Lancet. 1988, Vol. 1, 1309-11). The compounds of the present invention, being selective 5-HTι-like receptor agonists, are accordingly of particular use in the treatment of migraine and associated conditions, e.g. cluster headache, chronic paroxysmal he icrania, headache associated with vascular disorders, tension headache and paediatric migraine.

EP-A-0313397 and WO-A-91/18897 describe separate classes of tryptamine derivatives substituted by various five-membered heteroaliphatic rings, which are stated to be specific to a particular type of "5-HTι- like" receptor and thus to be effective therapeutic agents for the treatment of clinical conditions, particularly migraine, requiring this activity. However, neither EP-A-0313397 nor WO-A-91/18897 discloses or suggests the particular 4-substituted 1,2,4-triazole derivatives provided by the present invention.

EP-A-0497512, published on 5th August 1992, describes a class of substituted imidazole, triazole and tetrazole derivatives which are stated to be selective

agonists of 5-HTι-like receptors and hence to be of particular use in the treatment of migraine and associated conditions.

The present invention provides a compound of formula I:

( i ) wherein R represents a 2-(dimethylamino)ethyl group, or a group of formula (i) or (ii) :

( i ) (ϋ) or a salt or prodrug thereof.

The compounds of formula I above have interesting biological activity, being potent and highly selective agonists of 5-HT-ι-like receptors with good bioavailability. These compounds, and salts and prodrugs thereof, are generically encompassed within the scope of EP-A-0497512. However, EP-A-0497512 nowhere specifically discloses a 1,2,4-triazol-4-yl derivative, or a salt or prodrug thereof.

The compound of formula I above wherein R represents the group of formula (i) contains an asymmetric carbon atom at the 3-position of the pyrrolidine ring and is therefore optically active; for ease of reference, the relevant carbon atom has been designated by a "3\' ¹ symbol in formula (i) above. As a consequence of possessing an asymmetric carbon atom within the molecule, this compound can exist as (R) and (S) enantiomers. The present invention accordingly includes within its scope the individual enantiomers of this compound, as well as mixtures thereof. One such mixture, the so-called racemic mixture or racemate, contains equal proportions of the individual (R) and (S) enantiomers. In addition, mixtures of this compound containing at least 75% of the enantiomer wherein the carbon atom in the 3-position of the pyrrolidine ring is in either the (R) or the (S) configuration and 25% or less of the opposite enantiomer are provided by the present invention, as also are mixtures containing at least 85% of one enantiomer and 15% or less of the opposite enantiomer. Desirably, the mixture is enriched to the extent that it contains at least 95%, preferably at least 99%, of one enantiomer and no more than 5%, preferably no more than 1%, of the opposite enantiomer. For use in medicine, the salts of the compounds of formula I will be pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds according to the invention or of their pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds of this invention include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric

acid, sulphuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid.

The present invention includes within its scope prodrugs of the compounds of formula I above. In general, such prodrugs will be functional derivatives of the compounds of formula I which are readily convertible in vivo into the required compound of formula I. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.

Specific compounds within the scope of the present invention include:

(±)-N-methyl-3-[5-(1 , 2,4-triazol-4-yl)-lH-indol-3- y1]pyrrolidine;

3(R)-N-methyl-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]pyrrolidine of formula IA:

H

(IA)

3(S)-N-methyl-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]pyrrolidine of formula IB:

H-

(IB)

N-methyl-4-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]piperidine of formula IC:

(IC) N,N-dimethyl-2-[5-(1,2,4-triazol-4-yl)-lH-indol-3- yl]ethylamine of formula ID:

(ID) and salts and prodrugs thereof.

The invention also provides pharmaceutical compositions comprising one or more compounds of formula I above or a pharmaceutically acceptable salt thereof or a prodrug thereof in association with a pharmaceutically

acceptable carrier. Preferably these compositions are in unit dosage forms such as tablets, pills, capsules, powders, granules, sterile parenteral solutions or suspensions, metered aerosol or liquid sprays, drops, ampoules, auto-injector devices or suppositories; for oral, parenteral, intranasal, sublingual or rectal administration, or for administration by inhalation or insufflation. For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical carrier, e.g. conventional tableting ingredients such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, and other pharmaceutical diluents, e.g. water, to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention, or a pharmaceutically acceptable salt thereof. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid preformulation composition is then subdivided into unit dosage forms of the type described above containing from 0.1 to about 500 mg of the active ingredient of the present invention. The tablets or pills of the novel composition can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permits the inner component to pass

intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate.

The liquid forms in which the novel compositions of the present invention may be incorporated for administration orally or by injection include aqueous solutions, suitably flavoured syrups, aqueous or oil suspensions, and flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil, as well as elixirs and similar pharmaceutical vehicles. Suitable dispersing or suspending agents for aqueous suspensions include synthetic and natural gums such as tragacanth, acacia, alginate, dextran, sodium carboxymethylcellulose, methylcellulose, polyvinyl- pyrrolidone or gelatin.

In the treatment of migraine, a suitable dosage level is about 0.01 to 250 mg/kg per day, preferably about 0.05 to 100 mg/kg per day, and especially about 0.05 to 5 mg/kg per day. The compounds may be administered on a regimen of 1 to 4 times per day. The compounds of formula I above may be prepared by a process which comprises reacting the compound of formula II:

(I I) with a compound of formula III:

( I I I ) wherein R is as defined above; or a carbonyl-protected form thereof.

The reaction of compounds II and III may be carried out in a single step (Fischer indole synthesis) or by an initial non-cyclising step at a lower temperature to give a compound of formula IV:

( IV) wherein R is as defined above; followed by cyclisation using a suitable reagent, such as a polyphosphate ester, to give a compound of formula I.

The compound of formula I above wherein R represents the group of formula (i) may alternatively be prepared in racemic form by a process which comprises the following steps:

(A) reaction of the compound of formula II with a compound of formula V, or a carbonyl-protected form thereof:

(I I) (V)

wherein RP represents an amino-protecting group; to afford a compound of formula VI:

(VI)

wherein RP is as defined above;

(B) deprotection of the compound of formula VI thereby obtained, to afford a compound of formula VII:

H

(VI I) and

(C) methylation of the compound of formula VII thereby obtained.

As with that between compounds II and III, the reaction between compounds II and V may be carried out in a single step (Fischer indole synthesis) or by an initial non-cyclising step at a lower temperature to give a compound of formula VIII:

(VI I I ) wherein RP is as defined above; followed by cyclisation using a suitable reagent, such as a polyphosphate ester, to give a compound of formula VI.

Suitable examples of amino-protecting groups for the substituent RP include carboxylic acid groups such as chloroacetyl, trifluoroacetyl, for yl, benzoyl, phthaloyl, phenylacetyl or pyridinecarbonyl; acid groups derived from carbonic acid such as ethoxycarbonyl,

benzyloxycarbonyl, t-butoxycarbonyl, biphenylisopropoxy- carbonyl, p-methylbenzyloxycarbonyl, p-nitrobenzyloxy- carbonyl, p-bromobenzyloxycarbonyl, p-phenylazobenzyloxy- carbonyl, p-(p\'-methoxyphenylazo)benzyloxycarbonyl or t- amyloxycarbonyl; acid groups derived from sulphonic acid, e.g. p-toluenesulphonic acid; and other groups such as benzyl, p-methoxybenzyl, trityl, o-nitrophenylsulphenyl or benzylidene.

The removal of the protecting group present in the resultant compound may be effected by an appropriate procedure depending upon the nature of the protecting group. Typical procedures include hydrogenation in the presence of a palladium catalyst (e.g. palladium carbon or palladium black) for benzyloxycarbonyl, p-nitro- benzyloxycarbonyl, p-bromobenzyloxycarbonyl, p-phenylazo- benzyloxycarbonyl, p-(p\'-methoxyphenylazo)benzyloxy¬ carbonyl and trityl groups; treatment with hydrogen bromide in glacial acetic acid or trifluoroacetic acid for benzyloxycarbonyl, p-bromobenzyloxycarbonyl, p- phenylazobenzyloxycarbonyl and t-butoxycarbonyl groups; treatment with acetic acid and/or a mineral acid such as hydrochloric acid or sulphuric acid for trityl, t- butoxycarbonyl, formyl and benzylidene groups; and treatment with 2,3-dichloro-5,6-dicyano-l,4-benzoquinone for p-methoxybenzyl groups.

A particular amino-protecting group RP is benzyl. Where benzyl is employed as the amino-protecting group RP, a favoured method for its removal is hydrogenation. This may be conventional catalytic hydrogenation or, more particularly, the technique known as transfer hydrogenation. The latter procedure employs a hydrogenation catalyst such as palladium on carbon, ideally 10% palladium on carbon, in the presence of a hydrogen donor such as ammonium formate, sodium

hypophosphite, triethylammonium formate or potassium formate, preferably ammonium formate. Where ammonium formate is employed as the hydrogen donor, the reaction is conveniently carried out in a solvent such as methanol or aqueous methanol, advantageously at a temperature in the region of 35-45 ^β C.

The individual enantiomers of the compound of formula I above wherein R represents the group of formula (i) may be prepared by resolution of racemic N-methyl-3- [5-(1,2,4-triazol-4-yl)-lH-indol-3-yl]pyrrolidine, prepared as described above, or a protected derivative thereof which may subsequently be deprotected by methods known per se at an appropriate subsequent stage. Known methods of resolution may suitably be employed, for example comprising the formation and separation of diastereoisomers. Suitable resolving agents include chiral acids which form acid addition salts with amino groups within the molecule. Suitable resolving acids are camphor derivatives, such as camphor-10-sulphonic acid, α-bromo-camphor-w-sulphonic acid, hydroxymethylene camphor and camphoric acid; menthol derivatives such as menthoxyacetic acid; naturally occurring optically active forms of tartaric acid and malic acid; and diacetyltartaric acid. Alternatively, a chiral amino acid derivative may be employed in the resolution process, to form an amide bond, for example with the nitrogen atom at the 1- position of the indole nucleus, which subsequently may be cleaved under mild conditions. A suitable amino acid which may be employed is L-phenylalanine, optionally having its amino group protected.

The diastereoisomers are separated by conventional methods, such as chromatography or crystallisation. Suitable solvents for chromatography

include ethyl acetate and petroleum ethers. Suitable solvents for crystallisation include non-polar solvents such as ether, methylene dichloride, petroleum ethers and methanol.

After separation, the appropriate diastereoisomer is converted to the enantiomer wherein the carbon atom at the 3-position of the pyrrolidine ring is in the requisite configuration, either (R) or (S) as required. If necessary, the diastereoisomer obtained wherein the carbon atom at the 3-position of the pyrrolidine ring is in the opposite configuration may be re-racemised for further resolution.

The individual enantiomers of the compound of formula I above wherein R represents the group of formula (i) may also be prepared by a chiral process which comprises the following steps:

(i) reaction of the compound of formula II with a compound of formula IX, or a carbonyl-protected form thereof:

(IX) wherein the carbon atom designated * is in the (R) or (S) configuration; to afford a compound of formula X:

(X) wherein the carbon atom designated * is in the (R) or (S) configuration;

(ii) deprotection of the compound of formula X thereby obtained, to a afford a compound of formula XI:

(XI) wherein the carbon atom designated * is in the (R) or (S) configuration; and

(iii) methylation of the compound of formula XI thereby obtained.

Suitable carbonyl-protected forms of the compounds of formulae III, V and IX above include the dimethyl acetal derivatives.

As with that between compounds II and III, and between compounds II and V, the reaction between compounds II and IX may be carried out in a single step (Fischer indole synthesis) or by an initial non-cyclising

step at a lower temperature to give a compound of formula XII:

(XI I) wherein the carbon atom designated * is in the (R) or (S) configuration; followed by cyclisation using a suitable reagent, such as a polyphosphate ester, to give a compound of formula X.

The hydrazine derivative of formula II may be prepared from the corresponding aniline derivative of formula XIII:

(X I I I ) by diazotisation followed by reduction. Diazotisation is typically carried out using sodium nitrite/conc. HCl and the resulting diazo product reduced in situ using, for example, tin(II) chloride/cone. HCl, sodium sulphite/cone. HCl or sodium sulphite/cone. H2SO ₄ .

The aniline derivative of formula XIII may suitably be prepared by reacting the hydrazine derivative of formula XIV with the acetanilide of formula XV:

(XIV) (XV)

followed by removal of the N-acetyl protecting group. The reaction between compounds XIV and XV is conveniently effected in refluxing toluene, advantageously in the presence of a catalytic quantity of p-toluenesulphonic acid. Subsequent removal of the N- acetyl protecting group is typically effected in hot aqueous hydrochloric acid. The hydrazine derivative of formula XIV can be prepared from N,N\'-diformylhydrazine by reaction with thionyl chloride/N,N-dimethylformamide, as reported in J.

Chem. Soc. (C) . 1967, 1664, and subsequent treatment with sodium methoxide in methanol. The acetanilide of formula XV may be prepared by reduction of the corresponding nitro compound of formula XVI:

(XVI )

typically by transfer hydrogenation using a hydrogenation catalyst in the presence of a hydrogen donor such as ammonium formate, or alternatively by conventional catalytic hydrogenation or using tin(II) chloride.

The nitro compound of formula XVI is commercially available from Aldrich Chemical Company Ltd. , Gillingham, United Kingdom.

The preparation of the aldehyde of formula III above wherein R represents the group of formula (ii) is illustrated by the following reaction scheme:

(XVI I )

The starting compound XVII (l-methyl-4- piperidone) is commercially available from Aldrich

Chemical Company Ltd., Gillingham, U.K. Step 1 of the reaction scheme involves reacting this compound with the Horner-Emmons reagent Me0 ₂ C.CH2.PO(OEt)2 in the presence of sodium hydride, using THF as the solvent. In Step 2, the double bond of the resulting piperidine olefin ester is hydrogenated over palladium-charcoal in ethanolic HCl. This is followed in Step 3 by reduction of the side-chain methyl ester group using diisobutylaluminium hydride (DIBAL-H) in THF, with subsequent Swern oxidation of the

resulting terminal hydroxymethyl group to the aldehyde moiety present in the target intermediate of formula III.

The preparation of a typical intermediate of formula V above, wherein the amino-protecting group RP is benzyl, is illustrated by the following reaction scheme:

The starting compound XVIII (l-benzyl-3- pyrrolidinone) is commercially available from Aldrich Chemical Company Ltd., Gillingham, U.K. Step 1 of the reaction scheme involves reacting this compound with the Horner-Emmons reagent Meθ2C.CH ₂ .PO(OEt)2 in the presence of sodium hydride, using THF as the solvent. In Step 2, the double bond of the resulting pyrrolidine olefin ester is hydrogenated over palladium-charcoal in ethanolic HCl. This is followed in Step 3 by reduction of the side-chain methyl ester group using diisobutylaluminium hydride (DIBAL-H) in THF, with subsequent Swern oxidation of the resulting terminal hydroxymethyl group to the aldehyde moiety present in the target intermediate of formula V. The aldehyde derivatives of formula IX above may be prepared by reduction of the corresponding cyano compound of formula XIX:

(XIX) wherein the carbon atom designated * is in the (R) or (S) configuration. A suitable reducing agent for effecting this transformation is diisobutylaluminium hydride (DIBAL-H) , and the reaction is conveniently carried out in tetrahydrofuran as solvent. The preparation of both enantiomers of the cyano compound of formula XIX above is described in J. Med. Chem.. 1990, 33., 71.

Step (ii) of the above-described chiral process comprises the deprotection of the compound of formula X. Removal of the amino-protecting group is suitably effected by hydrogenation. This may be conventional catalytic hydrogenation or, more particularly, the technique known as transfer hydrogenation as described above. Step (C) and step (iii) of the above-described processes comprise the methylation of the compounds of formulae VII and XI respectively. This is suitably effected by conventional N-methylation techniques, such as by treatment of compound VII or compound XI with formaldehyde in the presence of a reducing agent such as sodium cyanoborohydride.

The following Examples illustrate the preparation of compounds according to the invention.

The ability of test compounds to bind to 5-HTι-like receptors was measured in membranes prepared from pig caudate using the procedure described in J. Neurosci. _f 1987, 1_, 894. Binding was determined using 2 nM 5-hydroxytryptamine creatinine sulphate,

5-[l,2- ³ H(N) ] as a radioligand. Cyanopindolol (100 nM) and mesulergine (100 nM) were included in the assay to block out 5-HTu and 5-HT binding sites respectively. The concentration of the compounds of the accompanying Examples required to displace 50% of the specific binding (IC50) is below 1 μM in each case.

The activity of test compounds as agonists of the 5-HTι-like receptor was measured in terms of their ability to mediate contraction of the saphenous vein of New Zealand White rabbits, using the procedure described in Arch. Pharm.. 1990, 342. 111. Agonist potencies were calculated as -logioECso (pECso) values, from plots of percentage 5-HT (1 μM) response against the concentration of the agonist. The compounds of the accompanying Examples were found to possess pECso values in this assay of not less than 5.0 in each case.

EXAMPLE 1

(±) N-Methyl-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3-vn pyrrolidine. 2.55 Oxalate

INTERMEDIATE 1

N-Benzyl-3-(formylmethyDpyrrolidire

a) N-Benzyl-3-(carbomethoxymethyl) pyrrolidine

Methyl diethylphosphonoacetate (26.9g, 0.128mol) in THF (50ml) was added dropwise to a stirred suspension of NaH (5.12g, 60% dispersion in oil, 0.128mol) in THF (125ml), at 10°C. The mixture was stirred for 0.6h and a solution of N-benzyl pyrrolidin-3-one (20.4g, 0.117mol) in THF (50ml) added dropwise. The mixture was heated at 50°C for 3h before removing the solvent under vacuum and redissolving the residue in CH _JJ C _J (300ml) and H ₂ 0 (100ml). The CH ₂ C1 ₂ phase was separated and washed with H ₂ 0 (50ml) and sodium bisulphite solution (2 x 50ml) and dried (Na ₂ S0 ₄ ). The crude product was chromatographed on silica gel eluting with petroleum ether/ ethyl acetate (60:40) to give a mixture of the unsaturated esters (24.7g, 92%). A solution of the preceding unsaturated ester (18.8g,

81.4mmol) in MeOH (95ml) and 2NHC1 (40ml) was hydrogenated at 50 psi, over Pd-C (1.9g), for 0.25h. The catalyst was removed by filtration through celite and the solvents removed under vacuum. The residue was basified with saturated K ₂ OO ₃ solution (100ml) and extracted with EtOAc (2x). The combined extracts were dried

(MgS0 ₄ ) and evaporated and the residue chromatographed on silica gel, eluting with CH^yMeOH (96:4) to give the title-

carbomethoxy ester (15.4g, 81%); δ (360MHz, CDC1 ₃ ) 1.40-1.49 (IH, m, CH of CH ₂ ): 2.03-2.12 (IH, m, CH of CH ₂ ), 2.18 (IH, dd, J=6.4 and 9.2Hz, CH of CH ₂ ), 2.40 (2H, d, J=7.5Hz, CH ₂ C0 ₂ CH ₃ ), 2.49-2.63 (3H, m, CH and CH ₂ ), 2.80 (IH, dd, J=7.6 and 9.2Hz, CH of CH ₂ ), 3.59 (2H, ABq, J=13Hz CH ₂ Ph), 3.65 (3H, s, CH ₃ ),

7.21-7.31 (5H, m, Ar-H).

b) N-Benzyl-3-(formylmethyl)pyrrolid ^: ιe

Dusobutylah ήnium hydride (105ml of a 1M solution in toluene, O.lOδmol) was added dropwise to a stirred solution of the preceding ester (7.0g, 30.0mmol) in toluene (400ml) at -35°C, over a 0.5h period. The solution was allowed to warm to room temperature, and stirred for 2h, before quenching by addition of methanol (10ml), 2NNaOH (5ml) and ΕLβ (5ml), sequentially.

The mixture was stirred for lh and the resulting precipitate removed by filtration through celite. The solvent was removed under vacuum to give the desired ethyl alcohol (5.65g, 92%). Dimethylsulphoxide (1.66ml, 23.4mmol) was added dropwise to a solution of oxalyl chloride (1.49g, 11.7mmol) in

CH ₂ C1 ₂ (130ml) at -75°C. The mixture was stirred for 0.25h before adding a solution of the preceding al < ~hol (2.0g, 9.76mmol) in CH ₂ C1 ₂ (30ml) and stirring for lh, at -75°C. Triethylamine (4.94g, 48.8mmol) was added and the reaction mixture warmed to 25°C and stirred for lh. Water (100ml) and

CH ₂ C1 ₂ (400ml) were added and the mixture basified with saturated K^CO _g solution. The aqueous ph∑ .__ e was separated and extracted with CH ₂ C1 ₂ (2x). The combined extracts were dried (MgSO ₄ ) and evaporated and the residue chromatographed on silica gel eluting with CH^ EtOH (9:1) to give the desired aldehyde (1.63g, 82%); δ (360MHz, CDC1 ₃ ) 1.41-1.50 and 2.07-2.17 (2H, m, CH ₂ ), 2.20 (IH, dd, J=5.9 and 9.1Hz, CH of CH ₂ ), 2.54-2.67 (5H, m, CH and 2 of CH ₂ ), 2.80 (IH, dd, J=7.3

and 9.1 Hz, CH of CH ₂ ), 3.60 (2H, ABq, J=13.0Hz, CEL _j ), 7.22-7.31 (5H, m, Ar-H), 9.74 (IH, t, J=1.6Hz, HCO).

INTERMEDIATE 2

4-( 1.2.4-Triazol-4-yl)phenylhvdrazine

A solution of 4\'-nitroacetanilide (5.0g, 27.8τnmol) in EtOH/EtOAc (160ml, 1:1), H ₂ O (15ml) and 5N HCl (5.6ml,

28.0mmol) was hydrogenated over 10% Pd-C (0.50g) at 50 psi for 0.25h. The catalyst was removed by filtration through celite and the solvents removed under vacuum. The free base was generated by dissolving the product in ΕLfi, basifying with 2N NaOH and extracting into EtOAc. The combined extracts were dried (MgS0 ₄ ) and evaporated to give the title-aniline (3.75g, 90%); δ (250MHz, CDCL/D^MeOH) 2.10 (3H, s, CH ₃ ), 6.68 (2H, d, J = 8.8Hz, Ar-H), 7.27 (2H, d, J = 8.8Hz, Ar-H).

b) ^-(l^^-Triazol^-vDacetanilide

A mixture of the preceding aniline (3.52g, 23.4mmol), N,N-dimethylformamide azine (3.33g, 23.4mmol; J. Chem. Soc. C. 1967, 1664) and p-toluenesulphonic acid monohydrate (0.223g, 1.17mmol), in anhydrous toluene (100ml), was heated at reflux for 17h. The beige coloured precipitate was filtered off and washed with toluene and CH ₂ C1 ₂ and dried under vacuum to give the desired triazole (4.29g, 91%); δ (250MHz, D ₄ -MeOH, d ₆ -DMSO) 2.14 (3H, s, CH ₃ ), 7.60 (2H, d, J = 8.8Hz, Ar-H), 7.78 (2H, d, J = 8.8Hz, Ar-H), 8.96 (2H, s, Ar-H).

c) 4 ^/ -(1.2.4-Triazol-4-vDaniline

A solution of the preceding acetanilide (4.91g, 24.3mmol) in 5N HCl (100ml) was heated at 125°C for 1.5h. The mixture

was cooled to 0°C, basified with cone, aqueous NaOH solution and extracted with CH ₂ C1 ₂ (x 5). The combined extracts were dried (MgS0 ₄ ) and evaporated and the residue chromatographed on silica-gel eluting with (80:8:1) to give the title-aniline (2.94g, 76%); δ (250MHz, CDC1 ₃ ) 3.80 (2H, s, NH ₂ ),

6.71 (2H, d, J = 8.8Hz, Ar-H), 7.08 (2H, d, J = 8.8Hz, Ar-H), 8.36 (2H, s, Ar-H).

d) 4 -(l _P 2.4-Triazol-4-yl)phenylhydrazine To a solution of the preceding aniline (1.60g, 9.99mmol) in cone. HCl/H^O (23ml and 3ml respectively) was added at -21°C, a solution of NaN0 ₂ (0.69g, 9.99mmol) in H ₂ 0 (8ml), at such a rate as to maintain the temperature below -10°C. The mixture was stirred for 0.3h and then filtered rapidly through a sinter, under vacuum. The filtrate was added to a cooled (-20°C) solution of

SnCl ₂ .2H ₂ 0 (9.02g, 40.0mmol) in cone. HCl (17ml). The mixture was stirred at -20°C for 0.25h and then at room temperature for 1.25h. The resulting solid was filtered off and washed with Et ₂ 0 and dried under vacuum. The crude product was dissolved in H ₂ 0, basified with cone aq. NaOH and extracted with EtOAc

(x5). The combined extracts were dried (MgSO ₄ ) and evaporated to afford the title-product (0.95g, 54%); δ (CDClg/D ₄ -MeOH) 3.98 (3H, br s, NH and NH ₂ ), 6.97 (2H, d, J=12.0Hz, Ar-H), 7.25 (2H, d, J=12.0Hz, Ar-H), 8.48 (2H, s, Ar-H).

(±)N-Benzyl-3-r5-(1.2.4-triazol-4-yl)lH-indol-3- yllpyrrolidine

A solution of Intermediate 2 (0.416g, 2.37mmol) and Intermediate 1 (0.4g, 1.96mmol), in 4% H ₂ S0 ₄ (45ml), was heated at reflux for 40h. The mixture was cooled to room temperature and CH ₂ C1 ₂ (100ml) added and the aqueous basified (pH 12/13) with saturated K ₂ C0 ₃ solution. The aqueous layer was separated and

extracted further with CH ₂ C1 ₂ (x5). The combined extracts were dried (MgSO ₄ ) and evaporated and the residue chromatographed on silica gel, eluting with CH ₂ Cl ₂ MeOH 9:1), to give the title- benzylpyrrolidine (0.183g, 22.5%); δ (250MHz, CDC1 ₃ ) 1.87-2.06 (IH, m, CH of CH^), 2.30-2.43 (IH, m, CH of CH,), 2.69-3.02 (4H, m, 2 of CH ₂ ), 3.57-3.68 (IH, m, CH), 3.71 (2H, ABq, J=13Hz, CIL _j Ph), 7.05-7.36 (7H, m, Ar-H), 7.46 (IH, d, J=8.5Hz, Ar-H), 7.78 (IH, d, J=2.0Hz, Ar-H), 8.46 (2H, s, Ar-H), 8.71 (IH, br s, NH).

(±) N-H-3-r5-(1.2.4-Triazol-4-vD-lH-indol-3-yllpyrrohdine

A mixture of the preceding benzylpyrrolidine (0.183g, 0.53mmol), ammonium formate (0.176g, 2.79mmol) and 10%Pd-C (0.183g), in MeOH (17ml), was stirred at room temperature for 0.25h and then at 70°C for 0.9h. The catalyst was removed by filtration through celite and the solvent removed under vacuum. The crude product was chromatographed on silica gel eluting with CH _jj CL _j /MeOH/NHg (20:8:1) to give the desired NH-pyrrolidine (99mg, 73%); δ (360MHz, D ₄ -MeOH) 1.82-1.95 and 2.16-2.30 (each IH, each m, CH ₂ ), 2.76-3.10 (3H, m, CH of CH ₂ and CH ₂ ), 3.24-3.50 (2H, m, CH of CH ₂ and CH), 7.16 (IH, s, Ar-H), 7.17 (IH, dd, J=1.5 and 8.4Hz, Ar-H), 7.42 (IH, d, J=8.4Hz, Ar-H), 7.69 (IH, d, J=1.5Hz, Ar-H), 8.80 (2H, s, Ar-H).

(±) N-Methyl-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yll pyrrolidine. 2.55 Oxalate

A solution of HCHO (35mg of a 38% w/v solution; 0.44mmol) in MeOH (8ml) was added to a stirred solution of the preceding amine (90mg, 0.36mmol), NaCNBH ₃ (28mg, 0.45mmol) and glacial acetic acid (0.05ml, 0.89mmol), in MeOH (8ml), at 0°C. The mixture was stirred at 0°C for 2h and then at

room temperature for 0.7h. Saturated K^COg solution (6ml) was added and the solvent removed under vacuum. The resulting residue was taken up into EtOAc (125ml) and washed with brine (x2). The combined aqueous was re-extracted with EtOAc (x2) and the combined extracts dried (MgS0 ₄ ) and evaporated. Flash chromatography of the residue, eluting with CHjCyMeOH/NHg (40:8:1), afforded the desired product (78mg, 82%) and the 2.55 oxalate salt prepared; mp 40°C (hygroscopic). Found: C, 48.84; H, 5.02; N, 13.60. C ₁₅ H ₁₇ N ₅ .2.5 (C ₂ H ₂ 0 ₄ ). O^H _j O. 0.03 (EtOH). 0.03 (Et ₂ 0) requires C, 48.51; H, 4.62; N, 14.02%. δ (360MHz,

D ₂ 0) 2.26-2.44 and 2.58-2.76 (each IH, each m, CH ₂ ), 3.01 and 3.02 (total 3H, each s, CH ₃ ), 3.22-4.16 (total 5H, 2 of CH ₂ and CH), 7.39 (IH, dd, J=1.5 and 8.6Hz, Ar-H), 7.46 and 7.49 (total IH, each s, Ar-H), 7.67 (IH, d, J=8.6Hz, Ar-H), 7.84 (IH, d, J=1.5Hz, Ar-H), 9.28 (2H, s, Ar-H).

EXAMPLE 2

3(S)-N-Methyl-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yl1 pyrrolidine. Benzoate

INTERMEDIATE 3

3(S)-N-r(R)-l-Phenylethyll-3-(formylmethyl) pyrrolidine

a) 3(S)-N-r(R)-l-Phenylethyll-3-(cvanomethyl)pyrrolidine

Prepared from 3(R)-N-[(R)-l-phenylethyl]-3-(hydroxymethyl) pyrrolidine by literature procedures (J. Med. Chem. 1990, d) _» 71).

b) 3(S)-N-r(R)-l-Phenylethvn-3-(formylmethyl)pyrrohdine

Diisobutylaluminium hydride (37.4ml of a 1M solution in toluene, 37.4mmol) was added to a solution of the preceding nitrile (4.0g, 18.7mmol), in THF (100ml), and the mixture stirred at room temperature for 3h. Ethyl acetate (40ml) and saturated NH C1 solution (30ml) were added and the mixture stirred for 0.25h before adding 4% H ₂ S0 ₄ (10ml) and allowing to stir for 0.5h. The mixture was basified with KgCOg solution and extracted with EtOAc (3x). The combined extracts were dried

(Na ₂ S0 ₄ ) and evaporated and the crude product chromatographed on silica gel eluting with CH ₂ Cl ₂ MeOH (9:1) to give the title aldehyde (2.3g, 57%); δ (360MHz, CDC1 ₃ ) 1.37 (3H, d, J = 6.6Hz, CHgCH), 1.37-1.48 (IH, m, CH of CH ₂ ), 2.02- 2.12 (2H, m, CH and CH of CH ₂ ), 2.39-2.46, 2.51-2.65 and 2.81-

2.85 (IH, 4H and IH respectively, each m, 3 of CH ₂ ) _> 3.21 (IH, q, J = 6.6Hz, CHCH ₃ ), 7.20-7.32 (5H, m, Ar-H).

3(S)-N-Methyl-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yll pyrrolidine. Benzoate.

The title compound was prepared from the hydrazine, Intermediate 2, and the aldehyde, Intermediate 3, using the procedures described for Example 1. The benzoate salt was prepared; mp 187-190°C. Found: C, 68.11; H, 6.13; N, 18.11.

C ₁₅ H ₁₇ N ₅ .C ₇ H ₆ O ₂ requires C, 67.85; H, 5.95; N, 17.98%. δ (360MHz, D ₂ 0) 2.26-2.44 and 2.58-2.76 (each IH, each m, CH ₂ ), 3.03 (3H, s, CH ₃ ), 3.22-4.16 (total 5H, 2 of CH ₂ and CH), 7.34 (IH, dd, J = 1.5 and 8.6Hz, Ar-H), 7.46-7.57 (total 4H, m, Ar-H), 7.65 (IH, d, J = 8.6Hz, Ar-H), 7.76 (IH, d, J = 1.5Hz, Ar-H), 7.86-

7.88 (2H, m, Ar-H), 8.82 (2H, s, Ar-H).

EXAMPLE 3

3(R)-N-Methyl-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yl1 pyrrolidine. Benzoate

The title compound was prepared from 3(R)-N-[(R)-1- phenylethyl]-3-(cyanomethyl)pyrrolidine and Intermediate 2 using the procedures described for Example 1. The benzoate salt was prepared; mp 188-189°C. Found: C, 68.12; H, 6.06; N, 18.10. C ₁₅ H ₁₇ N ₆ .C ₇ H ₆ O ₂ requires C, 67.85; H, 5.95; N, 17.98%. δ (360MHz, d ₆ -DMSO) 1.91-2.00 and 2.29-2.42 (each IH, each m, CH ₂ ), 2.42 (3H, s, CH ₃ ), 2.60-2.88 (total 3H, m, CH ₂ and CH of CH ₂ ), 3.14-3.17 and 3.58-3.68 (each IH, each m, CH of CH ₂ and CH), 7.31 (IH, dd, J = 1.5 and 8.6Hz, Ar-H), 7.34 (IH, d, J = 1.5Hz, Ar-H), 7.44-7.50 and 7.54-7.59 (total 4H, each m, Ar-H),

7.85 (IH, d, J = 1.5Hz, Ar-H), 7.93-7.95 (2H, m, Ar-H), 9.02 (2H, s, Ar-H).

EXAMPLE 4

N-Methyl-4-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yllpiperidi ne.

INTERMEDIATE 4

N-Methyl-4-(formylmethyl)piperidine

a) N-Methyl-4-(carbomethoxymethylidenyl)piperidine

Methyl diethylphosphonoacetate (88.69g, 0.422mol) was added dropwise to a stirred suspension of sodium hydride (18.56g, 60% dispersion in oil, 0.464mol) in THF (300ml) under nitrogen, at such a rate as to maintain the temperature below 30°C. The mixture was stirred for lh and a solution of

N-methyl-4-piperidinone (47.71g, 0.422mol) in THF (150ml) was added dropwise. The mixture was heated at 60°C for 4.5h before removing the solvent under vacuum and redissolving the residue in dichloromethane (300ml) and water (200ml). The dichloromethane phase was separated, washed successively with water (200ml) and saturated sodium bisulphite solution (2 x 70ml) and dried (MgS0 ₄ ). The crude product was chromatographed on silica gel, eluting with methanol/ether (5:95) to give the title-product (19.75g, 28%). *H NMR (250MHz, CDα ₃ ) δ 2.30 (3H, s, N-CH ₃ ), 2.35 (2H, t, J=6Hz, CH ₂ ), 2.40-2.50

(4H, m, 2 of CH ₂ ), 3.00 (2H, t, J=6Hz, CH _j ,), 3.70 (3H, s, C0 ₂ CH ₃ ), 5.65 (IH, s, vinyl CH).

b) N-Methyl-4-(carbomethoxymethyPpipeτHHine

A solution of the preceding unsaturated ester (19.5g, O.llδmol) in MeOH (140ml), H ₂ 0 (28ml) and 5N HCl (23.1ml, O.llδmol) was hydrogenated over 10% Pd-C (1.95g) at 40 psi for O.δh. The catalyst was removed by filtration through celite and the solvents removed under vacuum. The free base was generated by dissolving the residue in H ₂ 0 (70ml), basifying with saturated I^C0 ₃ solution and extracting into EtOAc. The combined extracts were dried (MgS0 ₄ ) and evaporated to give the title-ester (8.41g; 43%). Η NMR (250MHz, CDC1 ₃ ) δ 1.24-1.37 (2H, m, CH ₂ ), 1.69-1.81 (3H, m, CH ₂ and CH), 1.94 (2H, td,

J ll.9 and 2.2Hz, CH ₂ ), 2.23-2.26 (δH, m including s at δ 2.26, NCH ₃ and CH ₂ ), 2.82 (2H, br d, J=11.6Hz, CH ₂ ), 3.67 (3H, s, C0 ₂ Me).

c) N-Methyl-4-(2-hydroxyethyl)piperidine

Diisobutylaluminium hydride (120ml of a 1M solution in toluene, 0.120mol) was added dropwise to a stirred solution of

the preceding ester (8.19g, 0.047mol) in toluene (3δ0ml) at -3δ°C imder nitrogen. The solution was allowed to warm to room temperature over lh, before recooling to -30°C and quenching by addition of methanol (δml), water (δml) and 2N NaOH (δml), δ sequentially. The mixture was allowed to warm to room temperature and the resulting precipitate removed by filtration through celite. The solvent was removed under vacuum and the residue passed through a pad of alumina, eluting with methanol/dichloromethane (4:96) to give the title-product (δ.δlg, 10 82%). Η NMR (360MHz, CDCl ₃ ) δ 1.23-1.47 (3H, m, CH ₂ and

CH), l.δ2 (2H, q, J=6.6Hz, CH ₂ ), 1.69 (2H, br d, J=13.0Hz, CH _j ,), 1.91 (2H, td, J=ll.δ and 2.1Hz, CH _j ), 2.18 (3H, s, CH ₃ ), 2.83 (2H, br d, J=11.8Hz, CH _j ,), 3.69 (2H, t, J=6.6Hz, CH _j ).

lδ d) N-Methyl-4- ⁽ formy1τn ^p thyl)piperidine

Dimethylsulphoxide (6.56ml, 92.4mmol) was added dropwise to a stirred solution of oxalyl chloride (4.03ml, 46.2mmol) in dichloromethane (300ml) at -70°C under nitrogen.

20 The mixture was stirred for 0.2h before adding a solution of the preceding alcohol (δ.δlg, 38.δmmol) in dichloromethane (80ml) and stirring for lh at -70°C. Triethylamine (26.8ml, 192mmol) was added and the reaction mixture warmed to room temperature. Water and dichloromethane were added and the δ mixture basified with saturated K ₂ C0 ₃ solution. The aqueous phase was separated and extracted with dichloromethane (x 4) and the combined extracts dried (MgS0 ₄ ) and evaporated. The crude product was chromatographed on alumina, eluting with methanol/ dichloromethane (1:99) to afford the title-aldehyde 0 (3.68g, 69%); Η NMR (360MHz, CDC1 ₃ ) δ 1.35 (2H, qd, J=11.9 and 3.8Hz, CH ₂ ), 1.69-1.73 (2H, m, CH ₂ ), 1.81-2.00 (3H, m, CH ₂ and CH), 2.23 (3H, s, CH ₃ ), 2.35-2.38 (2H, m, CH ₂ ), 2.83 (2H, br d, J=11.9Hz, CH ₂ ), 9.78 (IH, t, J=2.0Hz, CHO).

N-Methyl-4-r5-(1.2.4-triazol-4-yl)-lH-indol-3-yllpiperidine.

5 A solution of the dihydrochloride salt of Intermediate 2

(2.11g, δ.δlmmol) and Intermediate 4 (l.Og, 7.09mmol) in 4% H ₂ S0 ₄ (100ml) was heated at reflux for 22h. The mixture was cooled to 0°C, basified with saturated I^C0 ₃ solution and extracted into EtOAc (δ x 200ml). The combined extracts were

10 dried (Na ₂ S0 ), evaporated and the residue chromatographed on silica gel, eluting with CH ₂ Cl ₂ MeOH NH ₃ (60:8:1), to give the title-triazole (l.Oδg, δ4%). The monobenzoate salt was prepared; m.p. 218-220°C. Found: C, 68.δ4; H, 6.12; N, 17.32. requires C, 68.47; H, 6.2δ; N, 17.36%. Η NMR (360MHz, D ₂ O) δ lδ 1.90-2.0δ (2H, m, CH ₂ ), 2.20-2.38 (2H, m, CH ₂ ), 2.9δ (3H, s,

CH ₃ ), 3.07-3.30 (3H, m, CH and CH ₂ ), 3.δ8-3.72 (2H, m, CH ₂ ), 7.26 (IH, dd, J=1.8 and 8.6Hz, Ar-H), 7.3δ (IH, s, Ar-H), 7.44- 7.61 (4H, m, Ar-H), 7.71 (IH, d, J=1.8Hz, Ar-H), 7.86-7.89 (2H, m, Ar-H), 8.94 (2H, s, Ar-H).

20

EXAMPLE 5

N.N-Dimethyl-2-r5-(1.2.4-triazol-4-yl)-lH-indol-3-vn e bylaminfi Benzoate. δ

A solution of the dihydrochloride salt of Intermediate 2 (1.50g, 6.04mmol) and 4-N,N-dimethylaminobutanal dimethylacetal (0.976g, 6.05mmol) in 4% aqueous sulphuric acid (120ml) was stirred at room temperature for 2h and then heated 0 at reflux for 40h. After cooling to room temperature, dichloromethane was added and the aqueous basified with saturated aqueous potassium carbonate solution. The aqueous was separated and extracted further with dichloromethane (x 3).

The combined organics were dried (MgS0 ₄ ), evaporated and the residue chromatographed on silica gel, eluting with CHgCyMeOH/NHg (60:8:1), to give the title-triazole (0.70g, 4δ%). The benzoate salt was prepared by addition of a solution δ of benzoic acid in diethyl ether to a solution of the triazole in methanol-diethyl ether. The solvent was removed under vacuum and the resultant product triturated with diethyl ether; mp 172-174°C. Found: C, 66.δ9; H, 6.28; N, 18.42. C _j aHaNgOa requires C, 66.δ3; H, 6.14; N, 18.55%). H NMR (360MHz, D ₂ 0) 10 δ 2.95 (6H, s, NMe ₂ ), 3.26 (2H, t, J = 7.4Hz, CH ₂ ), 3.50 (2H, t, J =

7.4Hz, CH ₂ ), 7.32 (IH, d, J = 6.8Hz, Ar-H), 7.46-7.5δ (4H, m, Ar-H), 7.63 (IH, d, J = δ.6Hz, Ar-H), 7.73 (IH, s, Ar-H), 7.88 (2H, d, J = 6.8Hz, Ar-H), 8.81 (2H, s, Ar-H).

lδ

EXAMPLE 6: Tablet Preparation

Tablets containing 1.0, 2.0, 25.0, 26.0, 50.0 and 100 mg, respectively, of the following compounds are prepared as illustrated below:

(±)-N-Methyl-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]pyrrolidine. 2.55 Oxalate 3(S)-N-Methyl-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]pyrrolidine. Benzoate

3(R)-N-Methyl-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]pyrrolidine. Benzoate

N-Methyl-4-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yljpiperidine. Benzoate

N,N-Dimethyl-2-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]ethylamine. Benzoate

TABLE FOR DOSES CONTAINING FROM 1-25 MG OF THE ACTIVE COMPOUND

Active Compound Microcrystalline cellulose Modified food corn starch Magnesium stearate

TABLE FOR DOSES CONTAINING FROM 26-100 MG OF THE ACTIVE COMPOUND

Amount (mg) Active Compound 26.0 50.0 100.0

Microcrystalline cellulose 52.0 100.0 200.0

Modified food corn starch 2.21 4.25 8.5

Magnesium stearate 0.39 0.75 1.5

All of the active compound, cellulose, and a portion of the corn starch are mixed and granulated to 10% corn starch paste. The resulting granulation is sieved, dried and blended with the remainder of the corn starch and magnesium stearate. The resulting granulation is then compressed into tablets containing l.o mg, 2.0 mg, 25.0 mg, 26.0 mg, 50.0 mg and 100 mg of the active ingredient per tablet.