Title:
5-OXOPROLINASE, 5-OXOPROLINASE GENE, AND METHOD FOR PRODUCING 5-OXOPROLINASE
Document Type and Number:
WIPO Patent Application WO/2014/126186
Kind Code:
A1
Abstract:
Provided is 5-oxoprolinase which can be used suitably in use applications such as the improvement of umami taste of a food or beverage, the separation of an optical isomer from a racemic body and the quantification of L-pyroglutamic acid.
5-Oxoprolinase originated from a microorganism belonging to the genus Aspergillus; and a 5-oxoprolinase gene encoding the 5-oxoprolinase. The 5-oxoprolinase according to the present invention can be collected from a culture which is produced by culturing a microorganism containing the 5-oxoprolinase gene or a microorganism capable of producing the 5-oxoprolinase in a culture medium.
5-Oxoprolinase can be provided, which is less likely to be affected by the change in a pH value compared with known enzymes, can be used in a broad pH range, can fully act in an intermediate temperature range in which the 5-oxoprolinase is highly stable, and has high affinity for substrates. The 5-oxoprolinase according to the present invention has such an advantageous property that the 5-oxoprolinase is highly safe in food/beverage processing use, because the 5-oxoprolinase is originated from a microorganism belonging to the genus Aspergillus which has long eating experience.
Inventors:
MANAKA JUNKO (JP)
NAKAHARA TAKEHARU (JP)
NAKAHARA TAKEHARU (JP)
Application Number:
PCT/JP2014/053426
Publication Date:
August 21, 2014
Filing Date:
February 14, 2014
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/09; A23L1/00; A23L1/305; A23L27/21; A23L27/24; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/78
Foreign References:
| JPS5937947B2 | 1984-09-12 | |||
| JPH08252075A | 1996-10-01 | |||
| JPH1066566A | 1998-03-10 |
Other References:
DATABASE NCBI 26 February 2014 (2014-02-26), "Oxoprolinase.", accession no. 2TY24
DATABASE NCBI 26 February 2014 (2014-02-26), NIERMAN, W. C.: "Aspergillus flavus NRRL3357 scf_1106286417242, whole genome shotgun sequence.", accession no. W _002477251
ZHAO, G. ET AL.: "Comparative proteome analysis of Aspergillus oryzae 3.042 and A. oryzae 100-8 strains: Towards the production of different soy sauce flavors", JOURNAL OF PROTEOMICS, vol. 75, 2012, pages 3914 - 3924
MOCHIZUKI, K.: "Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4- carboxylic acid", ARCHIVES OF MICROBIOLOGY, vol. 172, 1999, pages 182 - 185
DATABASE NCBI 26 February 2014 (2014-02-26), NIERMAN, W. C.: "Aspergillus flavus NRRL3357 scf_1106286417242, whole genome shotgun sequence.", accession no. W _002477251
ZHAO, G. ET AL.: "Comparative proteome analysis of Aspergillus oryzae 3.042 and A. oryzae 100-8 strains: Towards the production of different soy sauce flavors", JOURNAL OF PROTEOMICS, vol. 75, 2012, pages 3914 - 3924
MOCHIZUKI, K.: "Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4- carboxylic acid", ARCHIVES OF MICROBIOLOGY, vol. 172, 1999, pages 182 - 185
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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