APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING

Title:

APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING

Document Type and Number:

WIPO Patent Application WO/2020/086144

Kind Code:

A3

Abstract:

The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa are also disclosed.

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Inventors:

CHAIKIND BRIAN (US)
VAN ROSSUM HENDRIK M (US)
MILLER AARON (US)
PERKOVICH PAUL (US)
SZYJKA SHAWN (US)
PATEL KEDAR (US)

Application Number:

PCT/US2019/046555

Publication Date:

September 17, 2020

Filing Date:

August 14, 2019

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Assignee:

ZYMERGEN INC (US)

International Classes:

C07K19/00; C12N15/63; C12N15/62; C12N15/64; C12N15/66

Domestic Patent References:

WO2015200334A1

2015-12-30

Foreign References:

US20160201089A1

2016-07-14

Attorney, Agent or Firm:

POMERANZ, Marcelo C. et al. (US)

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