Title:
ACQUISITION METHOD FOR PLANT TRANSFORMED CELL
Document Type and Number:
WIPO Patent Application WO/2015/118640
Kind Code:
A1
Abstract:
The present invention relates to an acquisition method for a plant transformed cell. The present invention includes the following steps: (a) a step in which desired DNA and a first marker gene are both used to transform a plant cell; and (b) a step in which, from amongst the transformed plant cells obtained in step (a), a transformed cell having a chromosome into which the desired DNA is inserted and the first marker gene is not inserted is selected. There is no step in which positive selection using the first marker gene is used to remove a transformed cell having a chromosome into which only the desired DNA is inserted.
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Inventors:
HIEI YUKOH (JP)
KOMARI TOSHIHIKO (JP)
KOMARI TOSHIHIKO (JP)
Application Number:
PCT/JP2014/052765
Publication Date:
August 13, 2015
Filing Date:
February 06, 2014
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
International Classes:
C12N15/09; A01H1/00; C12N5/10
Other References:
KONDRAK M. ET AL.: "Generation of marker- and backbone-free transgenic potatoes by site- specific recombination and a bi-functional marker gene in a non-regular one-border agrobacterium transformation vector", TRANSGENIC. RES., vol. 15, no. 6, December 2006 (2006-12-01), pages 729 - 737, XP019454972
HASHIMOTO R.Y. ET AL.: "Negative selection driven by cytosine deaminase gene in Lycopersicon esculentum hairy roots", PLANT SCIENCE, vol. 141, no. 2, 22 February 1999 (1999-02-22), pages 175 - 181, XP055218228
GALLEGO M.E. ET AL.: "Positive-negative selection and T-DNA stability in Arabidopsis transformation", PLANT MOL. BIOL., vol. 39, no. 1, January 1999 (1999-01-01), pages 83 - 93, XP002193570
JAGANNATH A. ET AL.: "The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L.", MOLECULAR BREEDING, vol. 8, no. 1, August 2001 (2001-08-01), pages 11 - 23, XP002380250
DE BLOCK M. ET AL.: "The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters", THEORETICAL AND APPLIED GENETICS, vol. 95, July 1997 (1997-07-01), pages 125 - 131, XP055130682
HASHIMOTO R.Y. ET AL.: "Negative selection driven by cytosine deaminase gene in Lycopersicon esculentum hairy roots", PLANT SCIENCE, vol. 141, no. 2, 22 February 1999 (1999-02-22), pages 175 - 181, XP055218228
GALLEGO M.E. ET AL.: "Positive-negative selection and T-DNA stability in Arabidopsis transformation", PLANT MOL. BIOL., vol. 39, no. 1, January 1999 (1999-01-01), pages 83 - 93, XP002193570
JAGANNATH A. ET AL.: "The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L.", MOLECULAR BREEDING, vol. 8, no. 1, August 2001 (2001-08-01), pages 11 - 23, XP002380250
DE BLOCK M. ET AL.: "The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters", THEORETICAL AND APPLIED GENETICS, vol. 95, July 1997 (1997-07-01), pages 125 - 131, XP055130682
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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