ACRYLAMIDE-DEGRADING SELF-CLONING ASPERGILLUS ORYZAE

WIPO Patent Application WO/2013/115397

A1

Provided are self-cloning Aspergillus oryzae that expresses amidase without induction culturing and is highly degrading of acrylamide, and a method for reducing acrylamide in which the Aspergillus oryzae is used. Aspergillus oryzae that has been transfected in a state where there is, without induction culturing, expression of a gene that encodes a protein that has amidase activity and has a sequence which is hybridizable under stringent conditions with a gene that encodes a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1, or a nucleic acid molecule comprising a base sequence complementary to a nucleic acid molecule that encodes the polypeptide; a method for reducing acrylamide, the method comprising a step for treating acrylamide-containing matter with contact with the Aspergillus oryzae; and a method for producing a reduced-acrylamide food or beverage.

OZEKI KENJI (JP)
SANO MOTOAKI (JP)
IWAI KAZUYA (JP)
FUKUNAGA TAIJI (JP)
NARITA YUSAKU (JP)
TSUBOI HIROKAZU (JP)
BOGAKI TAKAYUKI (JP)

PCT/JP2013/052498

August 08, 2013

February 04, 2013

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UESHIMA COFFEE (JP)
OZEKI KK (JP)

C12N1/15; A23F5/24; A23L5/20; C12N15/09

WO2004037007A2

2004-05-06

JP2010183867A	2010-08-26
JP2010035449A	2010-02-18
JP2011092185A	2011-05-12
JP2011087572A	2011-05-06

GOMI K. ET AL.: "Cloning and molecular characterization of the acetamidase-encoding gene (amdS) from Aspergillus oryzae", GENE, vol. 108, no. 1, 1 December 1991 (1991-12-01), pages 91 - 98, XP000609074, DOI: doi:10.1016/0378-1119(91)90491-S

UNIUS PATENT ATTORNEYS OFFICE (JP)
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