Title:
Nε-ACYL-L-LYSINE-SPECIFIC AMINOACYLASE
Document Type and Number:
WIPO Patent Application WO/2010/067871
Kind Code:
A1
Abstract:
Disclosed are: an Nε-acyl-L-lysine-specific aminoacylase which enables the efficient synthesis of an Nε-acyl-L-lysine; and a method for synthesizing an Nε-acyl-L-lysine with high efficiency. Specifically disclosed are: an Nε-acyl-L-lysine-specific aminoacylase comprising an amino acid sequence depicted in SEQ ID NO:2 or SEQ ID NO:5; DNA encoding the Nε-acyl-L-lysine-specific aminoacylase, or a variant thereof; and a transformant carrying a recombinant DNA molecule comprising the DNA or the variant thereof.
Inventors:
TAKAKURA YASUAKI (JP)
NAKANISHI KAZUHIRO (JP)
SUZUKI SHUNICHI (JP)
NIO NORIKI (JP)
KOREISHI MAYUKO (JP)
IMAMURA KOREYOSHI (JP)
IMANAKA HIROYUKI (JP)
NAKANISHI KAZUHIRO (JP)
SUZUKI SHUNICHI (JP)
NIO NORIKI (JP)
KOREISHI MAYUKO (JP)
IMAMURA KOREYOSHI (JP)
IMANAKA HIROYUKI (JP)
Application Number:
PCT/JP2009/070769
Publication Date:
June 17, 2010
Filing Date:
December 11, 2009
Export Citation:
Assignee:
AJINOMOTO KK (JP)
UNIV OKAYAMA NAT UNIV CORP (JP)
TAKAKURA YASUAKI (JP)
NAKANISHI KAZUHIRO (JP)
SUZUKI SHUNICHI (JP)
NIO NORIKI (JP)
KOREISHI MAYUKO (JP)
IMAMURA KOREYOSHI (JP)
IMANAKA HIROYUKI (JP)
UNIV OKAYAMA NAT UNIV CORP (JP)
TAKAKURA YASUAKI (JP)
NAKANISHI KAZUHIRO (JP)
SUZUKI SHUNICHI (JP)
NIO NORIKI (JP)
KOREISHI MAYUKO (JP)
IMAMURA KOREYOSHI (JP)
IMANAKA HIROYUKI (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/80; C12P13/02
Domestic Patent References:
| WO2006088199A1 | 2006-08-24 |
Other References:
KOREISHI, M. ET AL.: "A novel epsilon-lysine acylase from Streptomyces mobaraensis for synthesis of Nepsilon-acyl-L-lysines.", J. AM. OIL CHEM. SOC., vol. 82, no. 9, September 2005 (2005-09-01), pages 631 - 637
DATABASE GENBANK [online] 31 October 2008 (2008-10-31), "Streptomyces coelicolor A3(2) complete genome; segment 5/29.", retrieved from http://www.ncbi.nlm. nih.gov/sviewer/viewer.fcgi?24413740:EMBL: 12797448 Database accession no. AL939108
KOREISHI, M. ET AL.: "Efficient Nepsilon- lauroyl-L-lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis.", J. BIOTECHNOL., vol. 141, no. 3-4, 20 May 2009 (2009-05-20), pages 160 - 165
KOREISHI, M. ET AL.: "A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-acyl-L-amino acids and N-acyl- peptides.", J. AGRIC. FOOD CHEM., vol. 54, no. 1, 11 January 2006 (2006-01-11), pages 72 - 78
DATABASE GENBANK [online] 20 January 2007 (2007-01-20), "Hypothetical protein SCO1424.", retrieved from http://www.ncbi.nlm.nih.gov/sviewer/viewer. fcgi?81624706:CAGE:14197168 Database accession no. Q9RKY3
DATABASE GENBANK [online] 31 October 2008 (2008-10-31), "Streptomyces coelicolor A3(2) complete genome; segment 5/29.", retrieved from http://www.ncbi.nlm. nih.gov/sviewer/viewer.fcgi?24413740:EMBL: 12797448 Database accession no. AL939108
KOREISHI, M. ET AL.: "Efficient Nepsilon- lauroyl-L-lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis.", J. BIOTECHNOL., vol. 141, no. 3-4, 20 May 2009 (2009-05-20), pages 160 - 165
KOREISHI, M. ET AL.: "A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-acyl-L-amino acids and N-acyl- peptides.", J. AGRIC. FOOD CHEM., vol. 54, no. 1, 11 January 2006 (2006-01-11), pages 72 - 78
DATABASE GENBANK [online] 20 January 2007 (2007-01-20), "Hypothetical protein SCO1424.", retrieved from http://www.ncbi.nlm.nih.gov/sviewer/viewer. fcgi?81624706:CAGE:14197168 Database accession no. Q9RKY3
Attorney, Agent or Firm:
TSUJII Koichi et al. (JP)
辻居 Koichi (JP)
辻居 Koichi (JP)
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