Title:
ADAPTATIONS FOR HIGH EFFICIENCY I-F3-CRISPR-CAS SYSTEMS FOR GUIDE RNA-DIRECTED TRANSPOSITION IN HUMAN CELLS
Document Type and Number:
WIPO Patent Application WO/2023/154826
Kind Code:
A3
Abstract:
Provided are compositions and methods for modifying DNA substrates. The compositions include modified I-F3 proteins for use in a CRISPR systems to modify a DNA substrate. The modified proteins include I-F3 TnsC, TniQ, TnsA, TnsB and fusion proteins containing TnsA and TnsB, Cas8, Cas5, Cas7, and Cas6 modified proteins. The CRISPR systems include a guide RNA. Protein modifications provide for a higher transposition frequency than unmodified I-F3 CRISPR systems.
Inventors:
PETERS JOSEPH E (US)
WINGO ROBERT (US)
PETASSI MICHAEL (US)
DEVER DANIEL (US)
WIENERT BEEKE (US)
WINGO ROBERT (US)
PETASSI MICHAEL (US)
DEVER DANIEL (US)
WIENERT BEEKE (US)
Application Number:
PCT/US2023/062327
Publication Date:
October 05, 2023
Filing Date:
February 09, 2023
Export Citation:
Assignee:
UNIV CORNELL (US)
GRAPHITE BIO INC (US)
GRAPHITE BIO INC (US)
International Classes:
C12N15/113; C12N9/22; C12N15/63; C12N15/90
Domestic Patent References:
| WO2021188553A1 | 2021-09-23 |
Foreign References:
| US20020188105A1 | 2002-12-12 |
Other References:
STELLWAGEN, A.E. CRAIG, N.L.: "Analysis of gain-of-function mutants of an ATP-dependent regulator of Tn7 transposition", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 305, no. 3, 19 January 2001 (2001-01-19), United Kingdom , pages 633 - 642, XP004469193, ISSN: 0022-2836, DOI: 10.1006/jmbi.2000.4317
Attorney, Agent or Firm:
WATT, Rachel et al. (US)
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