Title:
ADENO-ASSOCIATED VIRUS STRUCTURAL PLASMID CAPABLE OF IMPROVING ADENO-ASSOCIATED VIRUS TITER
Document Type and Number:
WIPO Patent Application WO/2024/046403
Kind Code:
A1
Abstract:
The present invention relates to the technical field of molecular biology, and in particular to an adeno-associated virus structural plasmid capable of improving adeno-associated virus titer, which is provided with a Rep gene expression cassette and a Cap gene expression cassette in sequence in the gene expression direction. The Rep gene expression cassette comprises a rep gene regulated and transcribed by a first promoter, and a transcription termination signal fragment is arranged behind the rep gene; the Cap gene expression cassette comprises a cap gene regulated and transcribed by a second promoter, wherein the first promoter comprises any one of a P5 promoter, an RSV promoter, an MMTV promoter, a UBC promoter, and a U6 promoter, and the second promoter comprises one or more of a CMV promoter, a CBh promoter, a CAG promoter, an EF1α promoter, and an SFFV promoter.
Inventors:
GU LENGTAO (CN)
XUAN CHUNLING (CN)
CHEN ZHEN (CN)
XUAN CHUNLING (CN)
CHEN ZHEN (CN)
Application Number:
PCT/CN2023/115991
Publication Date:
March 07, 2024
Filing Date:
August 31, 2023
Export Citation:
Assignee:
JIANGSU GENSCRIPT PROBIO BIOTECH CO LTD (CN)
International Classes:
C12N15/864; A61K35/76; A61K48/00; C12N15/35; C12N15/86; C12R1/93
Foreign References:
| GB2592751A | 2021-09-08 | |||
| US20230038295A1 | 2023-02-09 | |||
| CN112888426A | 2021-06-01 |
Other References:
VINCENT K. A. ET AL.: "Analysis of Recombinant Adeno-Associated Virus Packaging and Requirements for rep and cap Gene Products", JOURNAL OF VIROLOGY, vol. 71, no. 3, 31 March 1997 (1997-03-31), XP000881017, ISSN: 0022-538X
LI, JUAN ET AL.: "Role for Highly Regulated rep Gene Expression in Adeno-Associated Virus Vector Production", JOURNAL OF VIROLOGY, vol. 71, no. 7, 31 July 1997 (1997-07-31), XP002924496, ISSN: 0022-538X
LI, JUAN ET AL.: "Role for Highly Regulated rep Gene Expression in Adeno-Associated Virus Vector Production", JOURNAL OF VIROLOGY, vol. 71, no. 7, 31 July 1997 (1997-07-31), XP002924496, ISSN: 0022-538X
Attorney, Agent or Firm:
ZHONGLUN LAW FIRM (CN)
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