PREAMBLE OF INVENTION- This invention in particular describes the nature of the invention and the manner in which it is to be performed.

FIELD OF INVENTION- The present invention relates generally to a material and advanced method of making a bacterial based product that fix atmospheric nitrogen from air and makes available to plant.

PRIOR ART

In the existing system as given in WIPO Patent Application No. PCT/BR2002/000013, wherein, the invention relates to the method for increasing the rate of conversion of atmospheric nitrogen into ammonia in a microorganism of the genus Rhizobium, by increasing the intracellular level of an activator protein which is capable of activating the transcription of DNA of the microorganism encoding one or more proteins capable of effecting such conversion in the microorganism.

[i] In the existing system as given in European Patent Application No. EP0341736, wherein, the invention relates to a fertilizer of bacterial biomass and potassium magnesium fertilizer for fertilizing alpine pasture and ski slopes, and revitalising forest plantations..

In the existing system as given in United States Patent Application No. 20050056064, wherein, .the invention relates to the formulation of an organic agricultural fertilizer. This product meets the standards of the U.S. Department of Agriculture (USD A). The material used is municipal sludge from treated municipal plants. The raw material meets the guidelines of the United States Environmental Protection Agency (USEPA). The sludge is treated with various additives to eliminate pathogens. Globally acceptable additives are added to the base material to produce the fertilizer. The macronutrient materials include phosphates, potash, and nitrates. In addition, liquid ammonia is used to add nitrogen and eliminate pathogens..

In the existing system as given in United States Patent Application No. 6228806, wherein, the invention relates to Fertilizer compositions comprising: A) an effective quantity of an inorganic or organic fertilizer; and B) a quantity of beneficial microorganisms sufficient to further enhance plant growth when the fertilizer composition is applied to soil and/or · control pathogens in the soil, and methods for their use..

In the existing system as given in United States Patent Application No. 5728648, wherein, the invention relates to to an improved fertilizing composition of the type containing nitrogen, phosphorus and potassium, with the improvement being the addition of 1-methyl- 4-(l-methylethenyl)cyclohexene (i.e., limonene) as an insecticide and fungicide.

In .the existing system as given in United States Patent Application No. 6221634 , wherein the invention relates to Xylitol or D-xylulose is produced through direct fermentation frqm glucose by culturing a microorganism belonging to the genus Gluconobacter, Acetobacter or Frateuria, and having an ability to produce xylitol or D-xylulose in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.

In the existing system as given in United States Patent Application No. 7691630, wherein, the invention provides novel methods for improving plant quality and yield in the presence of pathogens. The method increases the levels of pathogenesis-related proteins, such as PR1 , phenylalanine ammonia lyase, or plant cell wall proteins such as hydroxyproline-rich glycoproteins, in a plant by contacting the plant with a plant systemic inducer and a reactive oxygen species wherein the amount of the reactive oxygen species is sufficient to increase the amount of the pathogenesis-related protein above the level induced by the plant systemic inducer in the absence of the reactive oxygen species. A preferred reactive oxygen species is peracetic acid; a preferred plant systemic inducer is salicylic acid.

PARENT CASE DATE:

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of application number 339/MUM/2010, filed February, 02, 2010, now pending.

BACKGROUND OF THE INVENTION

Potassium fulfills numerous vital functions in various processes in plants, animals and humans. For adequate nutrient supply of potassium, soil reserves are essentially required, which commonly contain more potassium than any other nutrient, including nitrogen.

For an adult human being, approximately 2 grams of potassium (K) is required per day, even though a typical person will take in 2.8-4.5 grams/day. There is no health risks associated with potassium. The rich sources of this nutrient in human diet are milk, fruit * juice, root vegetables and bananas.

Nitrogen; phosphorus, and potassium are three of the most essential nutrients that a plant needs to grow. Potash plays an important role in helping plants to absorb potassium required to thrive.

Potash has three main uses: fertilizer, livestock feed supplements and industrial processes. 95% of world's potash is used in fertilizers, while the rest is used for feed supplements and industrial production.

Potash is a key ingredient in fertilizers that enhances water retention of plants, increases crop yields and plants' disease resistance.

As is well known by any expert in the art, there have been hitherto made a number of proposals as to a method of producing organic fertilizer. To practice the conventional methods various kinds of raw materials and processes were employed but each of them has drawbacks in terms of procurement of raw material and cost. OBJECT OF THE INVENTION

Hence, the present invention has been made with the foregoing background in mind and its object resides in providing a material and method of rationally producing a high quality of potash mobilizing bio-fertilizer at the highest economical level using raw material which can be easily procured at the most inexpensive cost.

STATEMENT OF INVENTION-

The inventor has invented a composition and method for improving crop yields, involving potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia , which mobilizes the immobilized potash and produces plant growth promoting substances which benefit the plant in a multifaceted way.

DETAILED DESCRIPTION OF INVENTION-

The subject invention concerns materials and methods of preparing potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia. This is rich pota'sh immobilizers. This self-evolving formulation will bring you a new consortium every time.

It mobilizes the immobilized potash and produces plant growth promoting substances which benefit the plant in a multifaceted way.

Material and Method used in preparing the above said innovative combination is as under -

Step-1 Nucleus culture (Maintain culture) Pure culture of bacteria {Frateuria aurentia) is inoculated aseptically on plate having 20 ml PAC-010 Agar media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator at 27 ± 1 °C for 6 to 7 days with 12/12 hr lighting cycle. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO ₄ 7H ₂ O, 5 gm Yeast extract, 5 gm Peptone, 20 gm Agar agar and 1000 ml distilled water)

Step-2 Starter culture (In 100 ml flask)

Culture grown on plate is inoculated aseptically in a flask (250 ml capacity) having 100 ml PAC-010 broth media with the help of inoculating loop, and allow to grow at 27 ± 1 °C for at least 3 to 4 days. For superior growth, flask is put on shaker with agitation of media at 150 RPM. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO ₄ 7H ₂ O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water)

Step-3 Growth culture (In 1000 ml flasks

After sufficient growth in 250 ml flask (step-2), 50 ml culture from this grown culture is inoculated aseptically in 500 ml PAC-010 broth media in a 1000 ml capacity flask for further growth at 27 ± 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgS0 ₄ 7H ₂ 0, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water)

Step-4 Seed Fermentor (In small fermentor of 10 lit capacities)

One (1) lit grown culture in step-3 is inoculated aseptically in small fermenter having 10 litter PAC-010 broth media, and allow for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO ₄ 7H ₂ O, 5 gm Yeast extract, 5 gm Peptone and .1000 ml distilled water)

Step-5 Production Fermentor (In Big fermentor of 200 lit capacities)

Ten (10) lit culture grown in step-4 is inoculated aseptically in Big fermenter having 200 litter PAC-010 media (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO ₄ 7H ₂ O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water), and allowed for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM Step-6 Bacterial Separation

Final output from big fermentor is centrifuge by using advance centrifugation technology. By this separated bacteria are use for formulation of product whereas remaining liquid is throughout.

Step-7 Formulation

Recovered bacteria form centrifuge is mix in a set of ration with suitable carrier composed of zero TDS water etc and then homogenized it for equivalently of formulation.

Step-8 Packging

Material prepared as above is then packed in 100 ml, 250 ml and 500 ml packing by using bottle or pouch packing machine.

Step-09 - Distrubution to farmer, retailer, distributor etc

The final material prepared has to be used in following form:

Dose in one Acre: In case of Seed treatment add 100 ml per seed required per hector

For soil application add 100 ml to 250 ml per hector