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Title:
AFFINITY SEPARATION MATRIX FOR PURIFYING PROTEIN CONTAINING IMMUNOGLOBULIN κ CHAIN VARIABLE REGION
Document Type and Number:
WIPO Patent Application WO/2016/121701
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a peptide having excellent capacity for binding to a κ chain variable region of an antibody, an affinity separation matrix comprising this peptide as a ligand, and a method that is for producing a protein containing a κ chain variable region and that uses the affinity separation matrix. Another purpose of the present invention is to provide DNA that encodes the peptide, a vector containing the DNA, and a transformant that is transformed by the vector. The problem addressed by the present invention is solved by the use of a peptide containing all or part of a B5 domain of protein L derived from Peptostreptococcus magnus strain 312.

Inventors:
YOSHIDA SHINICHI (JP)
MURATA DAI (JP)
Application Number:
PCT/JP2016/052036
Publication Date:
August 04, 2016
Filing Date:
January 25, 2016
Export Citation:
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Assignee:
KANEKA CORP (JP)
International Classes:
C07K17/00; C07K1/22; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09
Domestic Patent References:
WO2005113584A12005-12-01
WO2015190457A12015-12-17
WO2015190458A12015-12-17
Foreign References:
CN1634990A2005-07-06
JPH07506573A1995-07-20
Other References:
KIHLBERG B. M. ET AL.: "Protein LG: A Hybrid Molecule with Unique Immunoglobulin Binding Properties", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 35, 1992, pages 25583 - 25588
SVENSSON H. G. ET AL.: "Protein LA, a novel hybrid protein with unique single-chain Fv antibody- and Fab-binding properties", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 258, no. 2, 1998, pages 890 - 896
GRAILLE M. ET AL.: "Complex between Peptostreptococcus magnus Protein L and a Human Antibody Reveals Structural Convergence in the Interaction Modes of Fab Binding Proteins", STRUCTURE, vol. 9, no. 8, 2001, pages 679 - 687
BOTTOMLEY S. P. ET AL.: "Cloning, expression and purification of Ppl-1, a kappa-chain binding protein, based upon protein L from Peptostreptococcus magnus.", BIOSEPARATION, vol. 5, no. 6, 1995, pages 359 - 367
"Capto(TM) L", August 2014 (2014-08-01), Retrieved from the Internet
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (JP)
Hisakazu Ueki (JP)
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