Title:
AGENT FOR ACTIVATING OR SUPPRESSING CELL-TO-CELL COMMUNICATION THROUGH FUNCTIONAL PROTEIN, ANTIBODY OR APTAMER, METHOD FOR SCREENING FOR SAID AGENT, FUSION PROTEIN, NUCLEIC ACID ENCODING SAID FUSION PROTEIN, RECOMBINANT VECTOR, TRANSFORMED HOST CELL, EXOSOME FUNCTION MODULATOR, AND EXOSOME
Document Type and Number:
WIPO Patent Application WO/2020/045349
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: an agent for activating or suppressing a cell-to-cell communication through a functional protein, which can modify a cell-to-cell communication; an antibody or an aptamer which can be used in the agent; a method for screening for the agent; a fusion protein capable of modifying an exosome; a nucleic acid encoding the fusion protein; a recombinant vector carrying the nucleic acid; a host cell transformed with the recombinant vector; an exosome function modulator comprising the fusion protein or the nucleic acid; and an exosome comprising the fusion protein. An agent for activating or suppressing a cell-to-cell communication through a functional protein, the agent comprising a substance capable of increasing or decreasing the expression of UBL3 gene or UBL3 protein or a substance capable of enhancing or inhibiting the activity of UBL3 protein. A method for screening for the agent by employing, as a measure, the occurrence of change in the expression of UBL3 gene or UBL3 protein by a candidate substance or the occurrence of change in the activity of UBL3 protein by a candidate substance. A fusion protein composed of UBL3 protein and a functional protein; a nucleic acid encoding the fusion protein; a recombinant vector carrying the nucleic acid; a host cell transformed with the recombinant vector; an exosome function modulator comprising the fusion protein or the nucleic acid; and an exosome including the fusion protein in such a state that the functional protein can exhibit the function thereof.
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Inventors:
SETOU MITSUTOSHI (JP)
AGETA HIROSHI (JP)
TSUCHIDA KUNIHIRO (JP)
AGETA HIROSHI (JP)
TSUCHIDA KUNIHIRO (JP)
Application Number:
PCT/JP2019/033323
Publication Date:
March 05, 2020
Filing Date:
August 26, 2019
Export Citation:
Assignee:
FUJITA ACAD (JP)
PREPPERS CO LTD (JP)
PREPPERS CO LTD (JP)
International Classes:
A61K45/00; A61K31/7088; A61K38/16; A61K39/395; A61P35/00; A61P43/00; C07K19/00; C12N15/11
Foreign References:
| US20110135596A1 | 2011-06-09 |
Other References:
UEDA, YOJI ET AL.: "Protein transport mechanism for exosome by means of modifier UBL3 after new translation", ABSTRACTS OF CONSORTIUM OF BIOLOGICAL SCIENCES, 2017
AGETA, H. ET AL.: "UBL3 modification influences protein sorting to small extracellular vesicles", NATURE COMMUNICATIONS, vol. 9, September 2018 (2018-09-01), pages 3936, XP055695922
DOWNES, P. B. ET AL.: "MUBs, a family of Ubiquitin- fold proteins that are plasma membrane-anchored by a prenylation", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 281, 2006, pages 27145 - 27157, XP055695927
AGETA, H. ET AL.: "UBL3 modification influences protein sorting to small extracellular vesicles", NATURE COMMUNICATIONS, vol. 9, September 2018 (2018-09-01), pages 3936, XP055695922
DOWNES, P. B. ET AL.: "MUBs, a family of Ubiquitin- fold proteins that are plasma membrane-anchored by a prenylation", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 281, 2006, pages 27145 - 27157, XP055695927
Attorney, Agent or Firm:
NIIYAMA Yuichi et al. (JP)
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