Title:
AGENTS THAT BIND TO AND INHIBIT HUMAN CYTOCHROME P450 2A6
Document Type and Number:
WIPO Patent Application WO2000004861
Kind Code:
A3
Abstract:
The invention provides monoclonal antibodies and other binding agents to human cytochrome P450 2A6 having advantageous properties, including capacity substantially to inhibit enzyme activity of human cytochrome P450 2A6 and lack of specific binding to other human cytochromes P450. The binding agents of the invention are useful inter alia in methods for screening drugs for metabolism by cytochrome P450 2A6, and in methods of measuring p450 2A6 levels in an individual relative to p450 2A6 levels in a control population.
Inventors:
GELBOIN HARRY V (US)
GONZALES FRANK J (US)
GONZALES FRANK J (US)
Application Number:
PCT/US1999/012380
Publication Date:
April 20, 2000
Filing Date:
July 19, 1999
Export Citation:
Assignee:
US GOV HEALTH & HUMAN SERV (US)
GELBOIN HARRY V (US)
GONZALES FRANK J (US)
GELBOIN HARRY V (US)
GONZALES FRANK J (US)
International Classes:
C07K16/40; C12P21/08; (IPC1-7): C07K16/00; C12P21/08
Other References:
PELKONEN ET AL.: "Coumarin 7-hydroxylase: characteristics and regulation in mouse and man", JOURNAL OF THE IRISH COLLEGES OF PHYSICIANS AND SURGEONS, vol. 22, no. 2, 1993, pages 24 - 28 (SUPPL. 1), XP002923291
GELBOIN ET AL.: "A monoclonal antibody inhibitory to human P450 2D6: a paradigm for use in combinatorial determination of individual P450 role in specific drug tissue metabolism", PHARMACOGENETICS, vol. 7, 1997, pages 469 - 477, XP002923292
GELBOIN ET AL.: "Inhibitory and non-inhibitory monoclonal antibodies to human cytochrome P450 3A3/4", BIOCHEMICAL PHARMACOLOGY, vol. 50, no. 11, 1995, pages 1841 - 1850, XP000644797
KRAUSZ ET AL.: "Inhibitory monoclonal antibodies to human cytochrome P450 2D6", BIOCHEMICAL PHARMACOLOGY, vol. 54, 1997, pages 15 - 17, XP002923293
YAMAZAKI ET AL.: "7-Ethoxycoumarin O-deethylation catalyzed by cytochromes P450 1A2 and 2E1 in human liver microsomes", BIOCHEMICAL PHARMACOLOGY, vol. 51, 1996, pages 313 - 319, XP002923294
VON MOLTKE ET AL.: "Phenacetin O-deethylation by human liver microsomes in vitro: inhibition by chemical probes, SSR1 antidepressants, nefazodone and venlafaxine", PSYCHOPHARMACOLOGY, vol. 128, 1996, pages 398 - 407, XP002923295
TASSANEEYAKUL ET AL.: "Specificity of substrate and inhibitor probes for human cytochromes P450 1A1 and 1A2", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 265, no. 1, 1992, pages 401 - 407, XP002923296
GELBOIN ET AL.: "A monoclonal antibody inhibitory to human P450 2D6: a paradigm for use in combinatorial determination of individual P450 role in specific drug tissue metabolism", PHARMACOGENETICS, vol. 7, 1997, pages 469 - 477, XP002923292
GELBOIN ET AL.: "Inhibitory and non-inhibitory monoclonal antibodies to human cytochrome P450 3A3/4", BIOCHEMICAL PHARMACOLOGY, vol. 50, no. 11, 1995, pages 1841 - 1850, XP000644797
KRAUSZ ET AL.: "Inhibitory monoclonal antibodies to human cytochrome P450 2D6", BIOCHEMICAL PHARMACOLOGY, vol. 54, 1997, pages 15 - 17, XP002923293
YAMAZAKI ET AL.: "7-Ethoxycoumarin O-deethylation catalyzed by cytochromes P450 1A2 and 2E1 in human liver microsomes", BIOCHEMICAL PHARMACOLOGY, vol. 51, 1996, pages 313 - 319, XP002923294
VON MOLTKE ET AL.: "Phenacetin O-deethylation by human liver microsomes in vitro: inhibition by chemical probes, SSR1 antidepressants, nefazodone and venlafaxine", PSYCHOPHARMACOLOGY, vol. 128, 1996, pages 398 - 407, XP002923295
TASSANEEYAKUL ET AL.: "Specificity of substrate and inhibitor probes for human cytochromes P450 1A1 and 1A2", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 265, no. 1, 1992, pages 401 - 407, XP002923296
Download PDF:
Previous Patent: ROLLING-BALL MASSAGER FOR HUMAN HEAD
Next Patent: BIOENHANCED FORMULATIONS COMPRISING EPROSARTAN IN ORAL SOLID DOSAGE FORM
Next Patent: BIOENHANCED FORMULATIONS COMPRISING EPROSARTAN IN ORAL SOLID DOSAGE FORM