Title:
ALKALINE PHOSPHATASE COMPOSITION AND PRODUCTION METHODS FOR DEPHOSPHORYLATED NUCLEIC ACID AND LABELLED NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2020/067122
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a high-quality composition containing alkaline phosphatase; a method for producing dephosphorylated nucleic acid using the composition; and a method for producing labelled nucleic acid using the composition. To achieve the purpose, the present invention provides a composition containing alkaline phosphatase, and a peptide fragment group (A) including two or more peptide fragments each including 5-50 consecutive amino acid residues selected from positions 501-578 of the amino acid sequence of SEQ ID NO: 5, wherein the ratio of the total contained amount of one or more of the peptide fragments with respect to the contained amount of the alkaline phosphatase satisfies formula (A): (XA/Y) × 100 ≤ 4.4000 (in the formula, XA represents the peak area value of the peptide fragment group (A) as calculated by an automatic integral technique from an extracted ion chromatogram obtained by LC-MS/MS analysis on the composition, and Y represents the peak area value of the alkaline phosphatase as calculated by an automatic integral technique from a chromatogram obtained by LC-UV analysis on the composition).
Inventors:
UEDA YOJI (JP)
TAKII YUKI (JP)
ITO MASATERU (JP)
YAGI MAI (JP)
TAKII YUKI (JP)
ITO MASATERU (JP)
YAGI MAI (JP)
Application Number:
PCT/JP2019/037520
Publication Date:
April 02, 2020
Filing Date:
September 25, 2019
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
International Classes:
C12N9/16; C12N15/10; C12N15/55; C12Q1/6876
Domestic Patent References:
| WO2012115023A1 | 2012-08-30 |
Foreign References:
| US20080098491A1 | 2008-04-24 | |||
| US20070148140A1 | 2007-06-28 | |||
| JPH10262674A | 1998-10-06 | |||
| US5705610A | 1998-01-06 | |||
| US6142266A | 2000-11-07 | |||
| US7037659B2 | 2006-05-02 | |||
| JPH10503841A | 1998-04-07 | |||
| JP3922454B2 | 2007-05-30 |
Other References:
POZIDIS C, BOURIOTIS V : "Preparation of high purity alkaline phosphatase from calf intestine using dye-ligand chromatography", BIOSEPARATION, vol. 5, no. 2, 30 November 1994 (1994-11-30), pages 89 - 93, XP009526669, ISSN: 0923-179X
See also references of EP 3858989A4
See also references of EP 3858989A4
Attorney, Agent or Firm:
NAGAI Hiroshi et al. (JP)
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