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Title:
ALKALINE PROTEASE MUTANT, GENE THEREOF, ENGINEERING BACTERIA THEREOF, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/134236
Kind Code:
A1
Abstract:
The present invention relates to an alkaline protease mutant, a gene thereof, an engineering bacteria thereof, a preparation method therefor and the use thereof, which belong to the technical field of bioengineering. The preparation method of the present invention comprises: extracting genome DNA from Bacillus clausii; performing PCR amplification to obtain a wild-type alkaline protease gene sequence; performing mutation by means of error-prone PCR on the wild-type alkaline protease gene obtained by amplification; performing high-throughput screening to obtain multiple alkaline protease genes having a high activity; performing DNA shuffling on the alkaline protease genes having a high activity; and screening same to obtain eight alkaline protease mutant genes having a higher activity.

Inventors:
LU FUPING (CN)
LIU YIHAN (CN)
LI YU (CN)
WANG XINGJI (CN)
WANG KEFEN (CN)
LIU WENLONG (CN)
LIU FUFENG (CN)
ZHANG HUITU (CN)
Application Number:
PCT/CN2021/071281
Publication Date:
June 30, 2022
Filing Date:
January 12, 2021
Export Citation:
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Assignee:
TIANJIN UNIV OF SCIENCE AND TECHNOLOGY (CN)
SHANDONG LONCT ENZYMES CO LTD (CN)
International Classes:
C12N9/54; C12N1/21; C12N15/10; C12N15/57; C12N15/75; C12R1/07; C12R1/10; C12R1/125
Foreign References:
CN112458072A2021-03-09
CN111334494A2020-06-26
CN105176951A2015-12-23
CN110819612A2020-02-21
CN106661566A2017-05-10
CN1537163A2004-10-13
Attorney, Agent or Firm:
BEIJING RISINGMARK INTELLECTUAL PROPERTY AGENCY (CN)
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