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Title:
AMINO ACID-BASED FORMULA AND PRODUCTION PROCESS THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/126215
Kind Code:
A1
Abstract:
The present invention relates to an amino acid-based formula comprising 18 amino acids, rice glucose polymer, sucrose, vegetable oil and optionally, additive and/or water, wherein the rice glucose polymer comprises glucose, maltose, maltotriose and glucose polymer having at least 4 glucose repeat units. The present invention also relates to the production process of an amino acid-based formula comprising the steps of: (i) preparing the rice glucose polymer by enzymatic hydrolysis of starch and (ii) preparing an amino acid-based formula wherein the starch : enzyme : water weight ratio in the preparation of rice glucose polymer by enzymatic hydrolysis of starch is 1 x 106: 1 : 5 x 106.

Inventors:
JIRAPINYO, Pipop (80-81 Soi Boromrajchonanee 48, Boromrajchonanee Road Chimple, Talingchan Bangkok, 10170, TH)
Application Number:
TH2015/000007
Publication Date:
August 11, 2016
Filing Date:
February 03, 2015
Export Citation:
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Assignee:
JIRAPINYO, Pipop (80-81 Soi Boromrajchonanee 48, Boromrajchonanee Road Chimple, Talingchan Bangkok, 10170, TH)
International Classes:
A23C11/00; A61K31/198
Domestic Patent References:
2001-08-09
2009-05-14
2007-01-25
Foreign References:
US20040022926A12004-02-05
JPH07100000A1995-04-18
Attorney, Agent or Firm:
CHANGCHUMNI, Manoon (Rouse & Co. International Limited, Unit 1401-3 and 1408 Floor 14, Two Pacific Place,142 Sukhumvit Roa, Klongtoey Bangkok, 10110, TH)
Download PDF:
Claims:
Claims

1. An amino acid-based formula comprising:

- 18 amino acids;

- rice glucose polymer;

- sucrose;

- vegetable oils; and

- optionally, additives and/or water

wherein the rice glucose polymer comprises glucose, maltose, maltotriose and glucose polymer' having at least 4 glucose repeat units.

2. An amino acid-based formula according to claim 1 wherein preferred rice glucose polymer comprises 5-10 % of glucose, 5-10 % of maltose, 5-10 % of maltotriose, 5-10 % of glucose polymer having at least 4 glucose repeat units, 5-10 % of glucose polymer having at least 5 glucose repeat units, 5-10 % of glucose polymer having at least 6 glucose "repeat units and 10-20 % of glucose polymer having more than 6 glucose repeat units by weight of the total rice glucose polymer.

3. An amino acid-based formula according to claim 1 or 2 wherein the total amount of the rice glucose polymer ranges from 40 - 60 wt% based on the total amount of the amino acid-based formula

4. An amino acid-based formula according to any one of claims 1-3 wherein the rice glucose polymer contain less than 0.05% of protein.

5. An amino acid-based formula according to any one of claims 1-4 wherein the rice glucose polymer is obtained from enzymatic hydrolysis of starch.

6. An amino acid-based formula according to claim 5 wherein the starch is preferably rice starch.

7. An amino acid-based formula according to claim 5 wherein the enzyme is amylase enzyme.

8. An amino acid-based formula according to claim 1 wherein 18 amino acids comprises histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, alanine, aspartic acid, cysteine, glutamic acid, glycine, proline, serine and tyrosine.

9. An amino acid-based formula according to claim 1 or 8 wherein the 18 amino acids preferably comprise 2.0-3.0 wt% of histidine, 5.0-7.0 wt% of isoleucine, 1.0-1.5 wt% of leucine, 6.0-9.0 wt% of lysine, 1.0-2.0 wt% of methionine, 3.0-5.0 wt% of phenylalanine, 4.0-5.5 wt% of threonine, 1.5-2.5 wt% of tryptophan, 5.0-7.5 wt% of valine, 3.0-4.5 wt% of arginine, 3.0-5.0 wt% of alanine, 8.5-11.0 wt% of aspartic acid, 2.0-3.0 wt% of cysteine, 20.0-24.5 wt% of glutamic acid, 2.0-3.0 wt% of glycine, 8.5-11.0 wt% of proline, 4.0-6.0 wt% of serine and 4.0-5.0 wt% of tyrosine based on the total amount of the 18 amino acids.

10. An amino acid-based formula according to any one of claims 1, 8 and 9 wherein the total amount' of 18 amino acids ranges from 12.0-16.5 wt% based on the total amount of the amino acid based-formula.

11. An amino acid-based formula according to claim 1 wherein the total amount of sucrose ranges from 10-15 wt% based on the total amount of the amino acid based-formula.

12. An amino acid-based formula according to claim 1 wherein vegetable oils are selected from a group consisting of palm oil, soybean oil, sunflower oil, coconut oil, rice bran oil and mixture thereof, preferably palm oil and soybean oil.

13. An amino acid-based formula according to claim 1 wherein the total amount of vegetable oils ranges from 14-27 wt% based on the total amount of the amino acid-based formula.

14. An amino acid-based formula according to claim 1 wherein the additives are selected from a group consisting of calcium lactate, calcium phosphate, magnesium oxide, sodium ferrous citrate, zinc sulfate, potassium iodide, sodium chloride, tripotassium citrate, copper sulfate, manganese sulfate, retinol, biotin, folate, lecithin, carnitine, selenium, taurine, inulin, fructo -oligosaccharides, vitamin C, vitamin D, vitamin E, vitamin K, vitamin Bls vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B12 and mixture thereof.

15. An amino acid-based formula according to claim 14 wherein the total amount of inulin and fructo -oligosaccharides ranges from 0.50-1.0 wt% based on the total amount of the amino acid-based formula

16. A production process of the amino acid-based formula comprising the steps of:

(i) preparing the rice glucose polymer by enzymatic hydrolysis of starch; and

(ii) preparing an amino acid-based formula wherein the starch : enzyme : water weight ratio in the preparation of rice glucose polymer by enzymatic hydrolysis of starch is 1 x 106: 1 : 5 x 106.

17. A production process of an amino acid-based formula according to claim 16 wherein the preparation of rice glucose polymer by enzymatic hydrolysis of starch is carried out in a temperature between 80-90 °C, preferably, 85 °C.

18. A production process of an amino acid-based formula according to claim 16 wherein the enzyme is amylase enzyme.

19. A production process of an amino acid-based formula according to claim 16 wherein the starch is rice starch.

20. A production process of an amino acid-based formula according to claim 16 wherein the preparation process of an amino acid-based formula comprises the steps of:

(a) mixing the rice glucose polymer from step (i) with clean water;

(b) adding sucrose, 18 amino acids and vegetable oils to the mixture solution obtained from step (a);

(c) homogenizing the blend obtained from step (b) to obtain a liquid amino acid-based formula; and

(d) optionally drying the liquid amino acid-based formula obtained from step (c) to obtain a powder amino acid-based formula.

21. A production process of an amino acid-based formula according to claim 20 further comprises the step of adding additives prior to step (d).

22. A production process of an amino acid-based formula according to claim 20 wherein 18 amino acids comprise histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, alanine, aspartic acid, aysteine, glutamic acid, glycine,, proline, serine and tyrosine.

23. A production process of an amino acid-based formula according to claim 20 wherein 18 amino acids preferably comprise 2.0-3.0 wt% of histidine, 5.0-7.0 wt% of isoleucine, 1.0-1.5 wt% of leucine, 6.0-9.0 wt% of lysine, 1.0-2.0 wt% of methionine, 3.0-5.0 wt% of phenylalanine, 4.0-5.5 wt% of threonine, 1.5-2.5 wt% of tryptophan, 5.0-7.5 wt% of valine,- 3.0-4.5 wt% of arginine, 3.0-5.0 wt% of alanine, 8.5-11.0 wt% of aspartic acid, 2.0-3.0 wt% of cysteine, 20.0-24.5 wt% of glutamic acid, 2.0-3.0 wt% of glycine, 8.5-11.0 wt% of proline, 4.0-6.0 wt% of serine and 4.0-5.0 wt% of tyrosine based on the total amount of the 18 amino acids.

24. A production process of an amino acid-based formula according to claim 20 wherein the total amount of the 18 amino acids ranges from 12.0-16.5 wt% based on the total amount of the amino acid-based formula.

25. An amino acid-based formula according to claim 20 wherein the vegetable oils are selected from a group consisting of palm oil, soybean oil, sunflower oil, coconut oil, rice bran oil and mixture thereof, preferably palm oil and soybean oil.

26. An amino acid-based formula according to claim 20 wherein the total amount of vegetable oils range from 14-27 wt% based on the total amount of the amino acid-based formula.

27. A production process of an amino acid-based formula according to claim 21 wherein the additives are selected from the group consisting of calcium lactate, calcium phosphate, magnesium oxide, sodium ferrous citrate, zinc sulfate, potassium iodide, sodium chloride, tripotassium citrate, copper sulfate, manganese sulfate, retinol, biotin, folate, lecithin, carnitine, selenium, taurine, inulin, fructo-oligo saccharides, vitamin C, vitamin D, vitamin E, vitamin K, vitamin Bi, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin Bi2 and mixture thereof.

28. A production process of an amino acid-based formula according to claim 16 or 20 wherein the rice glucose polymer comprises glucose, maltose, maltotriose and glucose polymer having at least 4 glucose repeat units or more also contains sucrose.

29. A production process of an amino acid-based formula according to claim 16 wherein the rice glucose polymer preferably comprises 5-10 % of glucose, 5-10 % of maltose, 5-10 % of maltotriose, 5-10 % of glucose polymer having at least 4 glucose repeat units, 5-10 % of glucose polymer having at least 5 glucose repeat units, 5-10 % of glucose polymer having at least 6 glucose repeat units and 10-20 % of glucose polymer having more than 6 glucose repeat units by weight of the total rice glucose polymer.

30. A production process of an amino acid-based formula according to claim 16 or 20 wherein the rice glucose polymer contains less than 0.05% of protein.

31. A production process of an amino acid-based formula according to claim 20 wherein the total amount of the rice glucose polymer ranges from 40-60 wt% based on the total amount of the amino acid-based formula.

32. A production process of an amino acid-based formula according to claim 20 wherein the total amount of sucrose ranges from 10-15 wt% based on the total amount of the amino acid based- formula.

Description:
AMINO ACID-BASED FORMULA AND PRODUCTION PROCESS THEREOF

FIELD OF THE INVENTION

Chemistry related to the amino acid-based formula and production process thereof.

BACKGROUND OF THE INVENTION

Cow milk allergy in infants caused by intolerance to certain proteins present in the cow milk is an important problem which can be commonly found. The number of infants suffering from such allergy has a tendency to constantly increase. The symptoms of cow milk allergy may vary, for example, atopic eczema, respiratory symptoms such as chronic cough or excessive sputum and gastrointestinal symtoms such as vomitting, diarrhea or stomachache, etc.

Nowadays, numerous formulas specialized for infants having cow milk protein intolerance have been invented and developed through several processes such as extensively hydrolyzed formulas or soy-based formulas, etc. However, such formulas still is allergenic to many infants. Therefore, the development of alternative formulas remains necessary to overcome the aforementioned problems. An example of such alternative formulas is amino acid- based formula. Hoever, although the commercially available amino acid-based formula can replace the common cow milk for infants having cow milk allergy, its affordability remains problematic to parents due to its high price.

SUMMARY OF THE INVENTION

The present invention relates to amino acid-based formula comprising 18 amino acids, rice glucose polymer, sucrose, vegetable oils and optionally, additives and/or water, wherein the rice glucose polymer comprises glucose, maltose, maltotriose and glucose polymer having at least 4 glucose repeat units.

The present invention also relates to the production process of amino acid-based formula comprising the steps of: (i) preparing rice glucose polymer by enzymatic hydrolysis of starch and (ii) preparing amino acid-based formula wherein the starch : enzyme : water weight ratio in the preparation of rice glucose polymer by enzymatic hydrolysis of starch is 1 x 10 6 : 1 : 5 x 10 6 .

The object of the present invention is to provide amino acid-based formula and the production process thereof to obtain an amino acid-based formula having the least long chain protein possible i.e. having the long hain protein less than 0.05%. Said rice glucose polymer is prepared by enzymatically hydrolysing starch using a particular technique which yields low protein rice glucose polymer which, when formulated with other ingredients such as 18 amino acids, vegetable oils, mineral salts and vitamins, will result in an amino acid-based formula suitable for infant consumption. Accordingly, said amino acid-based can be easily produced in a less costly manner.

DETAILED DESCRIPTION OF THE EMBODIMENTS

The invention will be further explained in detail as follows.

The present invention relates to amino acid-based formula and the production process thereof.

In one aspect of the present invention, the amino acid-based formula comprises 18 amino acids, rice glucose polymer, sucrose, vegetable oils and optionally, additives and/or water wherein rice glucose polymer comprises glucose, maltose, maltotriose and glucose polymer having at least 4 glucose repeat units.

Preferably, the rice glucose polymer comprises 5-10 % glucose, 5-10 % maltose, 5-10 % maltotriose, 5-10 % glucose polymer having at least 4 glucose repeat units, 5-10 % glucose polymer having at least 5 glucose repeat units, 5-10 % glucose polymer having at least 6 glucose repeat units and 10-20 % glucose polymer having more than 6 glucose repeat units by weight of the total rice glucose polymer. The rice glucose polymer containing protein in an amount of less than 0.05% is obtained by enzymatic hydrolysis of starch, preferably, by hydro lyzing rice starch using amylase enzymes.

The rice glucose polymer according to the present invention is present in the total amount ranging from 40-60 wt% based on the total amount of amino acid-based formula.

The 18 amino acids according to the present invention comprise histidine preferably in an amount of 2.0-3.0 wt%, isoleucine preferably in an amount of 5.0-7.0 wt%, leucine preferably in an amount of 1.0-1.5 wt%, lysine preferably in an amount of 6.0-9.0 wt%, methionine preferably in an amount of 1.0-2.0 wt%, phenylalanine preferably in an amount of 3.0-5.0 wt%, threonine preferably in an amount of 4.0-5.5 wt%, tryptophan preferably in an amount of 1.5-2.5 wt%, valine preferably in an amount of 5.0-7.5 wt%, arginine preferably in an amount of 3.0-4.5 wt%, alanine preferably in an amount of 3.0-5.0 wt%, aspartic acid preferably in an amount of 8.5-11.0 wt%, cysteine preferably in an amount of 2.0-3.0 wt%, glutamic acid preferably in an amount of 20.0-24.5 wt%, glycine preferably in an amount of 2.0-3.0 wt%, proline preferably in an amount of 8.5-11.0 wt%, serine preferably in an amount of 4.0-6.0 wt% and tyrosine preferably in an amount of 4.0-5.0 wt% based on the total amount of 18 amino acids. The total amount of said 18 amino acids according to the present invention ranges from 12.0-16.5 wt% based on the total amount of amino acid-based formula.

The total amount of said sucrose according to the present invention ranges from 10-15 wt% based on the total amount of amino acid-based formula.

The vegetable oils according to the present invention are selected from the group consisting of palm oil, soybean oil, sunflower oil, coconut oil, rice bran oil and mixture thereof, preferably palm oil and soybean oil.

The vegetable oils according to the present invention are present at the total amount ranging from 14-27 wt% based on the total amount of amino acid-based formula.

In addition, the additives are selected from the group consisting of calcium lactate, calcium phosphate, magnesium oxide, sodium ferrous citrate, zinc sulfate, potassium iodide, sodium chloride, tripotassium citrate, copper sulfate, manganese sulfate, retinol, biotin, folate, lecithin, carnitine, selenium, taurine, inulin, fructo -oligosaccharides, vitamin C, vitamin D, vitamin E, vitamin K, vitamin Bi, vitamin B 2 , vitamin B 3 , vitamin B5, vitamin B 6 , vitamin B 12 and mixture thereof.

Inulin and fructo-oligosaccharides according to the present invention are present at the total amount ranging from 0.50-1.0 wt% based on the total amount of amino acid-based formula.

In a further aspect of the present invention, the production process of amino acid-based formula comprises the steps of: (i) preparing the rice glucose polymer by enzymatic hydrolysis of starch and (ii) preparing amino acid-based formula.

The preparation of the rice glucose polymer from enzymatic hydrolysis of starch according to the present invention is carried out using the weight ratio of starch : enzyme : water of 1 x 10 6 : 1 : 5 x 10 6 (for example 20 kg : 20 mg : 100 kg). The enzymatic hydrolysis of starch is carried out at a temperature between 80-90 °C, preferably 85°C. The enzyme used is amylase enzyme and the starch is rice starch.

The preparation of the amino acid-based formula according to the present invention comprises the steps of:

(a) mixing rice glucose polymer from step of preparing the rice glucose polymer by enzymatic hydrolysis of starch with clean water;

(b) adding sucrose, 18 amino acids and vegetable oils to the mixture solution obtained from of step (a)

(c) homogenizing the blend obtained from step (b) to obtain a liquid amino acid-based formula; and (d) optionally drying the liquid amino acid-based formula obtained from step (c) to obtain a powder amino acid-based formula,

The preparation of the amino acid-based formula according to the present invention further comprises the steps of adding the additives prior to step (d).

The production process of amino acid-based formula according to the present invention use the amount of the aforementioned ingredients comprising 18 amino acids, rice glucose polymer, vegetable oil and optionally, additives as described above.

Examples

Rice glucose polymer preparation

Rice starch was hydro lysed using fungal amylase enzyme in the presence of clean water at the preferred temperature of 85°C for about half an hour. The obtained hydrolysate is then precipitated and the supernatant is used for preparing the amino acid-based formula. The rice glucose polymer obtained from this step may be made into powder prior to the preparation of amino acid-based formula.

Amino acid-based formula preparation

An exemplary preparation of amino acid-based formula comprises the following steps;

1. mixing 50 g of rice glucose polymer and 1.25g of sucrose with 1 L of clean water;

2. adding 18 amino acids in the total amount of 16 g to the mixture solution obtained

3. adding vegetable oils i.e. 10 g of palm oil, 10 g of soybean oil and 10 g of sunflower oil to the bled obtained from step 2;

4. homogenizing the the blend obtained from step 3 by blending;

5. adding additives, mineral salts as well as vitamins (as indicated in the table below) to the blend obtained from step 4 and further homogenizing

The obtained amino acid-based formula will be spray dried so as to render it in powder form. List of additives and the amount used in the preparation of the amino acid-based formula

Arnino acid-based formula allergy test in infants

A test of the amino acid-based formula prepared according to the present invention (A) was conducted on infants aged between 0-4 months in comparison with the commercially available arnino acid-based formula (B). 23 of 46 tested infants were found to be intolerant to said commercially available arnino acid-based formula. 7 infants were intolerant to both formulas A and B and 16 from the 23 infants did not show any signs of allergy to formula A.

The above results suggest that the amino acid-based formula prepared according to the present invention can efficiently replace cow milk and other specialized formulas.

It should be appreciated that the aforementioned ingredients, amount of the ingredients and steps of producing amino acid-based formula according to the present invention are not limited to the above detailed description. Various variations and modifications in terms of the ingredients, amount of the ingredients and steps of producing amino acid-based formula can be applied without departing from the spirit and the scope of the invention.

BEST MODE OF PRACTICING THE INVENTION

The best mode of practicing the present invention is as escribed in the above detailed description.