AMINO ACID QUANTIFICATION METHOD AND AMINO ACID QUANTIFICATION KIT

WIPO Patent Application WO/2019/198623

A1

The present invention addresses the problem of providing a method for selectively, easily, and highly sensitively quantifying amino acids of interest using an aminoacyl-tRNA synthetase (AARS), and an amino acid quantification kit. The present invention relates to an amino acid quantification method for quantifying an amino acid in a sample using an AARS, the method being characterized in that from an aminoacyl-AMP-AARS-tRNA complex having been formed, AA and at least one compound selected from the group consisting of AARS, AARS-tRNA complex and tRNA are released, and the resulting compounds are reused to form the aminoacyl-AMP-AARS-tRNA complex, so as to eventually obtain pyrophoric acid or other such reaction product subject to measurement in an amount exceeding the number of moles of tRNA and/or the amino acid in the reaction liquid. The present invention also relates to an amino acid quantification kit, or the like, serving to implement said method.

YAMADA KENTA (JP)
AOKI HIDEYUKI (JP)
NAKATSUKA TOMOKO (JP)
KIDA MIKIKO (JP)
SATO DAISUKE (JP)
TERADA TAKAHO (JP)
HIKIDA YASUSHI (JP)
YOKOYAMA SHIGEYUKI (JP)

PCT/JP2019/015061

October 17, 2019

April 05, 2019

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IKEDA FOOD RES CO LTD (JP)
RIKEN (JP)

C12Q1/25; C12Q1/48

WO2017170469A1	2017-10-05
WO2013118894A1	2013-08-15
WO2009064843A1	2009-05-22

JP2013198448A

2013-10-03

FORBES C. D. ET AL.: "A high-throughput competitive scintillation proximity aminoacyl-tRNA synthetase charging assay to measure amino acid concentration", ANALYTICAL BIOCHEMISTRY, vol. 363, 2007, pages 246 - 254, XP025611963, DOI: 10.1016/j.ab.2007.01.027

ABE Masahiro (JP)

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