Title:
AMINOPEPTIDASE FOR CATALYZED SYNTHESIS OF DIPEPTIDE AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2019/011192
Kind Code:
A1
Abstract:
Provided by the present invention are an aminopeptidase for catalyzed synthesis of dipeptide and a preparation method and application therefor. Said aminopeptidase is an amino acid sequence shown in SEQ ID NO: 2; For the preparation of the enzyme, first a genetic engineering stream for the enzyme is contructed, all genes are are synthesized into enzyme genetic segments, and the segments are linearly reorganized by using a restriction endonuclease Sal I and then granulated. The genetic engineering strain constructed in the present invention may catalyze histidine and β-alanine to efficiently synthesize carnosine.
Inventors:
HUANG KEXUE (CN)
ZHU JUN (CN)
WU FENG (CN)
WANG FENGFENG (CN)
ZHANG CHAO (CN)
HUA JUNGUO (CN)
YU YUKUI (CN)
XU FEI (CN)
ZHU JUN (CN)
WU FENG (CN)
WANG FENGFENG (CN)
ZHANG CHAO (CN)
HUA JUNGUO (CN)
YU YUKUI (CN)
XU FEI (CN)
Application Number:
PCT/CN2018/094838
Publication Date:
January 17, 2019
Filing Date:
July 06, 2018
Export Citation:
Assignee:
JIANGSU CHENGXIN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N9/48; C12N15/57; C12P17/10
Foreign References:
| CN107217048A | 2017-09-29 | |||
| JP2011139667A | 2011-07-21 |
Other References:
DATABASE NCBI [O] 14 September 2015 (2015-09-14), XP055567874, Database accession no. XP_013961656 .1
HEYLAND, J.: "Simple Enzymatic Procedure for L-carnosine Synthesis: Whole- cell Biocatalysis and Efficient Biocatalyst Recycling", MICROBIAL BIOTECHNOLOGY, vol. 3, no. 1, 31 December 2010 (2010-12-31), pages 74, XP055567859
HECK, T.: "Kinetic Analysis of L-Camosine Formation by beta-Aminopeptidases", ADVANCED SYNTHESIS & CATALYSIS, vol. 352, 9 February 2010 (2010-02-09), pages 407, XP055567858
HEYLAND, J.: "Simple Enzymatic Procedure for L-carnosine Synthesis: Whole- cell Biocatalysis and Efficient Biocatalyst Recycling", MICROBIAL BIOTECHNOLOGY, vol. 3, no. 1, 31 December 2010 (2010-12-31), pages 74, XP055567859
HECK, T.: "Kinetic Analysis of L-Camosine Formation by beta-Aminopeptidases", ADVANCED SYNTHESIS & CATALYSIS, vol. 352, 9 February 2010 (2010-02-09), pages 407, XP055567858
Attorney, Agent or Firm:
CHINA WISPRO INTELLECTUAL PROPERTY LLP. (CN)
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