Title:
AMPLIFICATION AND DIFFERENTIATION METHOD FOR PANCREATIC CELLS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/204061
Kind Code:
A1
Abstract:
Provided is an amplification method for pancreatic cells. The method comprises successively performing digestion treatment, resuspension treatment, discontinuous density gradient centrifugation treatment and amplification treatment on pancreatic tissue. The method uses mammalian pancreatic ducts as the source of pancreatic precursor-like cells, and removes islet cells and acinar cells in the resulting cell cluster after discontinuous density gradient centrifugation. The amplification medium used comprises a reprogramming material consisting of several small molecule compounds. Also provided are a differentiation method for the pancreatic precursor-like cells obtained by the amplification method and use thereof.
Inventors:
ZHANG HONGDAN (CN)
Application Number:
PCT/CN2021/085019
Publication Date:
October 14, 2021
Filing Date:
April 01, 2021
Export Citation:
Assignee:
SHANGHAI CELLIVER BIOTECHNOLOGY CO LTD (CN)
SHANGHAI CRYOWISE MEDICAL TECH CO LTD (CN)
SHANGHAI CRYOWISE MEDICAL TECH CO LTD (CN)
International Classes:
C12N5/071; C07K14/62; C12P21/02
Foreign References:
| CN111440761A | 2020-07-24 | |||
| CN103881962A | 2014-06-25 | |||
| CN108330099A | 2018-07-27 |
Other References:
DAN HUI-GUO , AN LI-LONG , XING XIAO-JUAN , ZHAI YAN-HUI , XIAO MEI: "Research Progress in the Adult Pancreatic Duct Stem Cell", GUANGDONG AGRICULTURAL SCIENCES, 25 May 2013 (2013-05-25), pages 234 - 236, XP055857058, ISSN: 1004-874X, DOI: 10.16768/j.issn.1004-874x.2013.10.006
FANGFANG ZHANG, DONGSHEN MA, TINGSHENG LIU, YU HONG LIU, JIAMIN GUO, JING SONG, QIONG WU, YI PAN, YANFENG ZHANG, CHANGYING GUO, CH: "Expansion and Maintenance of CD133-Expressing Pancreatic Ductal Epithelial Cells by Inhibition of Transforming Growth Factor-beta Signaling", STEM CELLS AND DEVELOPMENT, vol. 28, no. 18, 15 September 2019 (2019-09-15), pages 1236 - 1252, XP055857061, ISSN: 1547-3287, DOI: 10.1089/scd.2019.0087
See also references of EP 4063487A4
FANGFANG ZHANG, DONGSHEN MA, TINGSHENG LIU, YU HONG LIU, JIAMIN GUO, JING SONG, QIONG WU, YI PAN, YANFENG ZHANG, CHANGYING GUO, CH: "Expansion and Maintenance of CD133-Expressing Pancreatic Ductal Epithelial Cells by Inhibition of Transforming Growth Factor-beta Signaling", STEM CELLS AND DEVELOPMENT, vol. 28, no. 18, 15 September 2019 (2019-09-15), pages 1236 - 1252, XP055857061, ISSN: 1547-3287, DOI: 10.1089/scd.2019.0087
See also references of EP 4063487A4
Attorney, Agent or Firm:
SHANGHAI IFUTURE INTELLECTUAL PROPERTY LAW FIRM (CN)
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