Title:
AMPLIFICATION OF DISTANT NUCLEIC ACID TARGETS USING ENGINEERED PRIMERS
Document Type and Number:
WIPO Patent Application WO/2012/038049
Kind Code:
A3
Abstract:
The invention is a method of amplifying nucleic acids by synthesizing an engineered amplicon containing the sequence of interest, but omitting intervening sequences present in the template molecule. The method utilizes an "amplicon bridge" incorporated into the amplification primers. The length and content of the desired amplicon can be chosen by the operator and can contain unique regions for probe binding.
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Inventors:
JOHNSON JENNY A (US)
Application Number:
PCT/EP2011/004642
Publication Date:
May 31, 2012
Filing Date:
September 15, 2011
Export Citation:
Assignee:
ROCHE DIAGNOSTICS GMBH (DE)
HOFFMANN LA ROCHE (CH)
HOFFMANN LA ROCHE (CH)
International Classes:
C12Q1/68
Foreign References:
| JP2009165371A | 2009-07-30 |
Other References:
D. J. TURNER ET AL: "Long-range, high-throughput haplotype determination via haplotype-fusion PCR and ligation haplotyping", NUCLEIC ACIDS RESEARCH, vol. 36, no. 13, 27 June 2008 (2008-06-27), pages E82 - E82, XP055022274, ISSN: 0305-1048, DOI: 10.1093/nar/gkn373
CHA-AIM K ET AL: "Reliable fusion PCR mediated by GC-rich overlap sequences", GENE, ELSEVIER, AMSTERDAM, NL, vol. 434, no. 1-2, 1 April 2009 (2009-04-01), pages 43 - 49, XP025990384, ISSN: 0378-1119, [retrieved on 20081229], DOI: 10.1016/J.GENE.2008.12.014
CHA-AIM K ET AL: "Reliable fusion PCR mediated by GC-rich overlap sequences", GENE, ELSEVIER, AMSTERDAM, NL, vol. 434, no. 1-2, 1 April 2009 (2009-04-01), pages 43 - 49, XP025990384, ISSN: 0378-1119, [retrieved on 20081229], DOI: 10.1016/J.GENE.2008.12.014
Attorney, Agent or Firm:
ROCHE DIAGNOSTICS GMBH (Mannheim, DE)
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