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Title:
ΑLPHA AMYLASE VARIANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/144008
Kind Code:
A1
Abstract:
Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100°C.

Inventors:
FAN, Yan (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
DU, Xiuzhen (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
HAO, Minghui (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
SUN, Yan (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
HUANG, Ke (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
LI, Feng (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
Application Number:
CN2017/074724
Publication Date:
August 31, 2017
Filing Date:
February 24, 2017
Export Citation:
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Assignee:
NANJING BESTZYME BIO-ENGINEERING CO., LTD. (Room 4120, Building 1 28 Yongxi Road, Science Park, Jiangnin, Nanjing Jiangsu 0, 211100, CN)
International Classes:
C12N9/28; C12N1/21; C12N15/56; C12N15/75; C12P19/04; C12P19/14; C12R1/10
Domestic Patent References:
WO2013184577A12013-12-12
Foreign References:
CN103781910A2014-05-07
CN102388132A2012-03-21
Other References:
WENDY A. OFFEN ET AL.: "Three-dimensional structure of a variant 'Termamyl-like' Geobacillus stearothermophilus a-amylase at 1. 9A° resolution", ACTA CRYSTALLOGRAPHICA SECTION F STRUCTURAL BIOLOGY COMMUNICATIONS, 1 January 2015 (2015-01-01), pages 66 - 70, XP055412007, ISSN: 2053-230X
Attorney, Agent or Firm:
NANJING TIANHUA PATENT AGENT CO., LTD. (Floor 23, Block A1 World Trade Center Building, No. 67 Shanxi Road, Gulo, Nanjing Jiangsu 9, 210009, CN)
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