Title:
ANAEROBIC MICROBIAL CULTURE AND REAL-TIME OBSERVATION DEVICE
Document Type and Number:
WIPO Patent Application WO/2022/199145
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of microbial culture, and provides an anaerobic microbial culture and real-time observation device, and a culture method for observing anaerobic microorganisms in real time by using the device. The device comprises a base, an upper lid, a micro-fluidic chip and a culture solution storage chamber, wherein the base and the upper lid are snap-fitted with each other in a sealed manner; a first slot is used for accommodating the culture solution storage chamber; a second slot is used for providing a culture space for anaerobic microorganisms; a third slot is used for accommodating the micro-fluidic chip, and a horizontal slot face of the third slot is provided with a first window; a fourth slot is disposed opposite the second slot, and a fifth slot is disposed opposite the first slot; a horizontal slot face of the fourth slot is provided with a second window; the first window and the second window are arranged opposite each other; and the culture solution storage chamber is provided with a liquid output hole, and a side wall of the upper lid is provided with a gas inlet, a gas outlet and a liquid outlet. The device has excellent gas tightness, and can provide a stable anaerobic gas atmosphere for long-term stable liquid culture of anaerobic microorganisms, and the gas atmosphere is controllable; and experimental requirements such as high throughput, large-range multi-site sampling and high-magnification photography are met.
Inventors:
HUANG SHUQIANG (CN)
YU YUE (CN)
MA ZHIXIN (CN)
DENG YUFANG (CN)
WEN HUI (CN)
LI SIHONG (CN)
YU YUE (CN)
MA ZHIXIN (CN)
DENG YUFANG (CN)
WEN HUI (CN)
LI SIHONG (CN)
Application Number:
PCT/CN2021/137926
Publication Date:
September 29, 2022
Filing Date:
December 14, 2021
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12M1/00; C12M1/04; C12M1/34
Foreign References:
CN112113901A | 2020-12-22 | |||
US20200181555A1 | 2020-06-11 | |||
US20200032186A1 | 2020-01-30 | |||
CN214654823U | 2021-11-09 | |||
CN112899124A | 2021-06-04 | |||
CN211847935U | 2020-11-03 |
Attorney, Agent or Firm:
BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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