Title:
ANALYSIS METHOD FOR GLYCAN HAVING ACID GROUP
Document Type and Number:
WIPO Patent Application WO/2019/098213
Kind Code:
A1
Abstract:
[Problem] To provide a column for column chromatography used to isolate an acidic glycan. [Solution] A column in which a second column is connected, by means of a flow passage, to downstream of the discharge opening of a first column, and which is selected from either (1) or (2): (1) the support in the first column is a hydrophobically-modified silica having a group which contains a primary amine, a secondary amine, and/or a tertiary amine, and the support in the second column is a resin having a group which contains a primary amine, a secondary amine, and/or a tertiary amine; and (2) the support in the first column is a resin having a group which contains a primary amine, a secondary amine, and/or a tertiary amine, and the support in the second column is a hydrophobically-modified silica having a group which contains a primary amine, a secondary amine, and/or a tertiary amine.
Inventors:
FUKATSU TOMOKI (JP)
MOROOKA CHIKA (JP)
MOROOKA CHIKA (JP)
Application Number:
PCT/JP2018/042064
Publication Date:
May 23, 2019
Filing Date:
November 14, 2018
Export Citation:
Assignee:
JAPAN CHEM RES (JP)
International Classes:
G01N30/88; B01J20/281; B01J20/283; B01J20/285; G01N30/02; G01N30/06; G01N30/46; G01N30/74; C07K16/00
Foreign References:
| JP2008309699A | 2008-12-25 | |||
| JP2015091953A | 2015-05-14 | |||
| JPH09127090A | 1997-05-16 | |||
| JP2010169691A | 2010-08-05 | |||
| JP2005241389A | 2005-09-08 | |||
| JP2005091953A | 2005-04-07 |
Other References:
DEGUCHI, K. ET AL.: "Two-dimensional hydrophilic interaction chromatography coupling anion-exchange and hydrophilic interaction columns for separation of 2-pyridylamino derivatives of neutral and sialylated N-glycans", JOURNAL OF CHROMATOGRAPHY A, vol. 1189, 2008, pages 169 - 174, XP022614225
NAKAGAWA, HIROAKI: "Structural analysis of oligosaccharides by a 2 or 3-dimensional mapping method", JAPANESE JOURNAL OF CLINICAL CHEMISTRY, vol. 34, no. 4, 2005, pages 319 - 325, XP055609378
KONDO, A. ET AL.: "Separation of Pyridylamino Oligosaccharides by High-Performance Liquid Chromatography on an Amine-Bearing Silica Column", ANALYTICAL BIOCHEMISTRY, vol. 219, 1994, pages 21 - 25, XP024763016
IKEGAMI, TOORU ET AL.: "A Perspective of Hydrophilic Interaction Chromatography-Development and the Characteristics of the separation mode", CHROMATOGRAPHY, vol. 29, no. 2, 2008, pages 1 - 6, XP009520314, ISSN: 1342-8284
KURAYA N., J. BIOCHEM., vol. 112, 1992, pages 122 - 6
KONDO A., AGRIC. BIOL. CHEM., vol. 54, 1990, pages 2169 - 70
See also references of EP 3712609A4
NAKAGAWA, HIROAKI: "Structural analysis of oligosaccharides by a 2 or 3-dimensional mapping method", JAPANESE JOURNAL OF CLINICAL CHEMISTRY, vol. 34, no. 4, 2005, pages 319 - 325, XP055609378
KONDO, A. ET AL.: "Separation of Pyridylamino Oligosaccharides by High-Performance Liquid Chromatography on an Amine-Bearing Silica Column", ANALYTICAL BIOCHEMISTRY, vol. 219, 1994, pages 21 - 25, XP024763016
IKEGAMI, TOORU ET AL.: "A Perspective of Hydrophilic Interaction Chromatography-Development and the Characteristics of the separation mode", CHROMATOGRAPHY, vol. 29, no. 2, 2008, pages 1 - 6, XP009520314, ISSN: 1342-8284
KURAYA N., J. BIOCHEM., vol. 112, 1992, pages 122 - 6
KONDO A., AGRIC. BIOL. CHEM., vol. 54, 1990, pages 2169 - 70
See also references of EP 3712609A4
Attorney, Agent or Firm:
YAMAMOTO, Yoshiki (JP)
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