Title:
ANALYSIS METHOD OF PROTEIN DISULFIDE BOND PAIRING
Document Type and Number:
WIPO Patent Application WO/2016/155620
Kind Code:
A1
Abstract:
Disclosed is an analysis method of protein disulfide bond pairing. According to a protein molecular structure to be analyzed, peptide fragments suitable for mass spectrometry are generated by enzymolysis of the protein with a specific protease, and thus characterization of disulfide bond linkage can be conformed in a simple, efficient and accurate way. The method is good in repeatability and simple in spectrum interpretation, can confirm the peptide fragments of disulfide bond linkage in protein molecules accurately and comprehensively, and has great suitability for quality monitoring of larger batches in industrial production.
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Inventors:
LV FENGHUA (CN)
TAN QINGQIAO (CN)
ZHANG QIANQIAN (CN)
HUAN MINXIA (CN)
HUANG LIMING (CN)
LIU ZHOUYANG (CN)
TAN QINGQIAO (CN)
ZHANG QIANQIAN (CN)
HUAN MINXIA (CN)
HUANG LIMING (CN)
LIU ZHOUYANG (CN)
Application Number:
PCT/CN2016/077810
Publication Date:
October 06, 2016
Filing Date:
March 30, 2016
Export Citation:
Assignee:
SUNSHINE GUOJIAN PHARMACEUTICAL (SHANGHAI) CO LTD (CN)
International Classes:
G01N33/68; C12Q1/37
Foreign References:
| US20110151493A1 | 2011-06-23 |
Other References:
ZHANG, WEI ET AL.: "Complete Disulfide Bond Assignment of a Recombinant Immunoglobulin G4 Monoclonal Antibody", ANALYTICAL BIOCHEMISTRY, vol. 311, 31 December 2002 (2002-12-31), pages 1 - 9, XP027266810
WYPYCH, J. ET AL.: "Human IgG2 Antibodies Display Disulfide-Mediated Structural Isoforms", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 23, 6 June 2008 (2008-06-06), pages 16194 - 16205, XP002512404
CHELIUS, D. ET AL.: "Reversed-Phase Liquid Chromatography in-Line with Negative Ionization Electrospray Mass Spectrometry for the Characterization of the Disulfide-Linkages of an Immunoglobulin Gamma Antibody", AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 17, 14 August 2006 (2006-08-14), pages 1590 - 1598, XP027973775
FLINN, J.P . ET AL.: "Role of Disulfide Bridges in the Folding, Structure and Biological Activity of omega-Conotoxin GVIA", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1434, 31 December 1999 (1999-12-31), pages 177 - 190, XP004278786
QI, JIANFENG ET AL.: "Determination of the Disylfide Structure of Sillucin, a Highly Knotted Cysteine-Rich Peptide, by Cyanylation/Cleavage Mass Mapping", BIOCHEMISTRY, vol. 40, no. 15, 21 March 2001 (2001-03-21), pages 4531 - 4538, XP055082994
WYPYCH, J. ET AL.: "Human IgG2 Antibodies Display Disulfide-Mediated Structural Isoforms", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 23, 6 June 2008 (2008-06-06), pages 16194 - 16205, XP002512404
CHELIUS, D. ET AL.: "Reversed-Phase Liquid Chromatography in-Line with Negative Ionization Electrospray Mass Spectrometry for the Characterization of the Disulfide-Linkages of an Immunoglobulin Gamma Antibody", AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 17, 14 August 2006 (2006-08-14), pages 1590 - 1598, XP027973775
FLINN, J.P . ET AL.: "Role of Disulfide Bridges in the Folding, Structure and Biological Activity of omega-Conotoxin GVIA", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1434, 31 December 1999 (1999-12-31), pages 177 - 190, XP004278786
QI, JIANFENG ET AL.: "Determination of the Disylfide Structure of Sillucin, a Highly Knotted Cysteine-Rich Peptide, by Cyanylation/Cleavage Mass Mapping", BIOCHEMISTRY, vol. 40, no. 15, 21 March 2001 (2001-03-21), pages 4531 - 4538, XP055082994
Attorney, Agent or Firm:
LECOME INTELLETUAL PROPERTY AGNET LTD. (CN)
北京律诚同业知识产权代理有限公司 (CN)
北京律诚同业知识产权代理有限公司 (CN)
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