Title:
ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2015/119137
Kind Code:
A1
Abstract:
[Problem] To identify and quantify triglyceride molecule types in a sample using few standards. [Solution] The present invention includes: a step for measuring the fatty acid composition of all fatty acids configuring triglycerides contained in a sample; a step for calculating for each molecule type the probability of appearance of a triglyceride molecule type contained in the sample from the measured fatty acid composition; a step for forming an actual chromatography profile by separating the triglyceride molecule types contained in the sample by means of chromatography; and a step for forming a predicted chromatography profile from the calculated probability of appearance of the triglyceride molecule types and a retention factor for each triglyceride molecule type. By comparing the predicted chromatography profile and the actual chromatography profile, the molecule type of each separated triglyceride molecule type is identified.
Inventors:
KOTANIGUCHI MIYAKO (JP)
KITAMURA SHINICHI (JP)
NAKAYA MAKOTO (JP)
KITAMURA SHINICHI (JP)
NAKAYA MAKOTO (JP)
Application Number:
PCT/JP2015/053065
Publication Date:
August 13, 2015
Filing Date:
February 04, 2015
Export Citation:
Assignee:
IPE INC (JP)
International Classes:
G01N30/88; G01N30/86
Foreign References:
| JP2005083952A | 2005-03-31 |
Other References:
WM. CRAIG BYRDWELL ET AL.: "Quantitative Analysis of Triglycerides Using AtmosphericPressure Chemical Ionization-Mass Spectrometry", LIPIDS, vol. 31, no. 9, 1996, pages 919 - 935, XP055219680
YASUHIRO FUNADA ET AL.: "Development of a simulation program for the analysis of oils and fats by subcritical fluid chromatography", ANALYTICA CHIMICA ACTA, vol. 401, no. 12, 1999, pages 73 - 82, XP055219684
DAMIANI P ET AL.: "Prediction of isocratic nonaqueous reversed-phase high-performance liquid chromatography retention parameters and response factors of triacylglycerols detected by an ultraviolet-diode array-evaporative light-scattering on-line system", JOURNAL OF CHROMATOGRAPHIC SCIENCE, vol. 38, no. issue 5, 2000, pages 195 - 199, XP055219689
YASUHIRO FUNADA ET AL.: "Development of a simulation program for the analysis of oils and fats by subcritical fluid chromatography", ANALYTICA CHIMICA ACTA, vol. 401, no. 12, 1999, pages 73 - 82, XP055219684
DAMIANI P ET AL.: "Prediction of isocratic nonaqueous reversed-phase high-performance liquid chromatography retention parameters and response factors of triacylglycerols detected by an ultraviolet-diode array-evaporative light-scattering on-line system", JOURNAL OF CHROMATOGRAPHIC SCIENCE, vol. 38, no. issue 5, 2000, pages 195 - 199, XP055219689
Attorney, Agent or Firm:
SHIMURA HISASHI (JP)
Shoji Shimura (JP)
Shoji Shimura (JP)
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