Title:
ANALYTE DETECTION METHOD AND REAGENT KIT FOR DETECTING ANALYTE
Document Type and Number:
WIPO Patent Application WO/2017/138497
Kind Code:
A1
Abstract:
Provided is an analyte detection method comprising: a step for forming an immune complex on a first solid phase by contacting a first solid phase with an analyte, a labeled antibody, and a capture antibody; a step for releasing the immune complex by dissociating the bond between the capture antibody and the first solid phase, and contacting the immune complex with a second solid phase that binds the capture antibody, thereby transferring the immune complex onto the second solid phase; and a step for detecting the analyte by measuring the label present in the complex on the second solid phase. The analyte detected by this method is a multimeric antigen, in particular amyloid-β or tau protein.
Inventors:
WATANABE TOSHIHIRO (JP)
Application Number:
PCT/JP2017/004271
Publication Date:
August 17, 2017
Filing Date:
February 06, 2017
Export Citation:
Assignee:
SYSMEX CORP (JP)
International Classes:
G01N33/543; G01N33/53
Domestic Patent References:
| WO2009011500A2 | 2009-01-22 | |||
| WO2014123131A1 | 2014-08-14 |
Foreign References:
| JP2008530578A | 2008-08-07 | |||
| JP2009085685A | 2009-04-23 | |||
| JP2001116571A | 2001-04-27 | |||
| JP2015502551A | 2015-01-22 | |||
| JP2001305137A | 2001-10-31 | |||
| JP2681370B2 | 1997-11-26 | |||
| JP2009517652A | 2009-04-30 | |||
| JP5164971B2 | 2013-03-21 |
Other References:
LEVINE H III: "Alzheimer's beta-peptide oligomer formation at physiologic concentrations", ANAL BIOCHEM, vol. 335, no. 1, 1 December 2004 (2004-12-01), pages 81 - 90, XP004616778
See also references of EP 3415912A4
See also references of EP 3415912A4
Attorney, Agent or Firm:
NOGAWA Shintaro (JP)
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