Title:
ANALYTE EXTRACTION APPARATUS AND METHOD
Document Type and Number:
WIPO Patent Application WO/2012/049491
Kind Code:
A3
Abstract:
Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.
Inventors:
PETTIGREW DAVID MICHAEL (GB)
LIU BO (GB)
LAITENBERGER PETER GEORG (GB)
BATES STEPHEN (GB)
LIU BO (GB)
LAITENBERGER PETER GEORG (GB)
BATES STEPHEN (GB)
Application Number:
PCT/GB2011/051958
Publication Date:
June 07, 2012
Filing Date:
October 11, 2011
Export Citation:
Assignee:
SPHERE MEDICAL LTD (GB)
PETTIGREW DAVID MICHAEL (GB)
LIU BO (GB)
LAITENBERGER PETER GEORG (GB)
BATES STEPHEN (GB)
PETTIGREW DAVID MICHAEL (GB)
LIU BO (GB)
LAITENBERGER PETER GEORG (GB)
BATES STEPHEN (GB)
International Classes:
B01D11/02; G01N30/06; G01N33/15
Domestic Patent References:
| WO2010017151A1 | 2010-02-11 | |||
| WO2005048126A2 | 2005-05-26 |
Other References:
MCGAUGHRAN L ET AL: "Rapid Measurement of Blood Propofol Levels: a Proof of Concept Study", JOURNAL OF CLINICAL MONITORING AND COMPUTING, KLUWER ACADEMIC PUBLISHERS, DO, vol. 20, no. 5, 24 August 2006 (2006-08-24), pages 381 - 381, XP019435587, ISSN: 1573-2614, DOI: 10.1007/S10877-006-9038-8
COHEN ET AL: "Quantitative measurement of propofol and in main glucuroconjugate metabolites in human plasma using solid phase extraction-liquid chromatography-tandem mass spectrometry", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 854, no. 1-2, 25 June 2007 (2007-06-25), pages 165 - 172, XP022127455, ISSN: 1570-0232, DOI: 10.1016/J.JCHROMB.2007.04.021
COHEN ET AL: "Quantitative measurement of propofol and in main glucuroconjugate metabolites in human plasma using solid phase extraction-liquid chromatography-tandem mass spectrometry", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 854, no. 1-2, 25 June 2007 (2007-06-25), pages 165 - 172, XP022127455, ISSN: 1570-0232, DOI: 10.1016/J.JCHROMB.2007.04.021
Attorney, Agent or Firm:
ELKINGTON AND FIFE LLP (8 Pembroke RoadSevenoaks, Kent TN13 1XR, GB)
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