Title:
ANIMAL CELLS HAVING CHIMERIC RECEPTOR AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2004/029244
Kind Code:
A1
Abstract:
It is intended to provide animal cells selectively inducing an immune response in the presence of an antigen which is specifically recognized by a single chain antibody, a vector DNA carrying a recombinant gene for constructing the above animal cells, and a method for producing a useful substance, a method of measuring the concentration of an antigen, a method of screening a cell response activator, a method of measuring an interaction between proteins, a method of controlling a cell response, a method of screening a chimeric receptor, a method of screening a cell having a foreign gene transferred thereinto, a virus vector DNA for treating cancer and so on with the use of the animal cells or the vector DNA as described above. Using fluorescein, a vector DNA carrying a recombinant gene of a single chain antibody 31IJ3 gene against thereto, a mouse erythropoietin receptor gene and a mouse gp130 gene is transferred into target cells and a chimeric receptor is expressed on the surface of cell membrane. Thus, transgenic cells, which can undergo cell growth factor-independent proliferation due to the stimulus by a fluorescein dimer, can be constructed.
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Inventors:
NAGAMUNE TERUYUKI (JP)
KAWAHARA MASAHIRO (JP)
UEDA HIROSHI (JP)
SHINKAI MASASHIGE (JP)
KAWAHARA MASAHIRO (JP)
UEDA HIROSHI (JP)
SHINKAI MASASHIGE (JP)
Application Number:
PCT/JP2003/012264
Publication Date:
April 08, 2004
Filing Date:
September 25, 2003
Export Citation:
Assignee:
NAGAMUNE TERUYUKI (JP)
KAWAHARA MASAHIRO (JP)
UEDA HIROSHI (JP)
SHINKAI MASASHIGE (JP)
KAWAHARA MASAHIRO (JP)
UEDA HIROSHI (JP)
SHINKAI MASASHIGE (JP)
International Classes:
G01N33/48; A61K35/12; A61K35/76; A61K48/00; A61P35/00; C07K16/44; C12N5/10; C12N15/09; C12P1/00; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/566; G01N33/574; (IPC1-7): C12N15/09; C12N5/10; C12Q1/02; G01N33/53; G01N33/566; G01N33/574
Domestic Patent References:
| WO1998038324A2 | 1998-09-03 |
Other References:
KAWAHARA, M. ET AL.: "selection of highly Productive Mammalian cells Based on an Inducible Growth advantage Using an Antibody/receptor chimera", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 93, no. 4, April 2002 (2002-04-01), pages 399 - 404, XP002977424
FITZER-ATTAS, C. J. ET AL.: "harnessing Syk family tyrosine kinases as signaling domains for chimeric single chain of the variable domain receptors: optimal design for T cell activation", J. IMMUNOL.,, vol. 160, no. 1, 1998, pages 145 - 154, XP002977425
FITZER-ATTAS, C. J. ET AL.: "direct T cell activation by chimeric single chain Fv-Syk promotes Syk-Cbl association and Cbl phosphorylation", J. BIOL. CHEM, vol. 272, no. 13, 1997, pages 8551 - 8557, XP002977426
ALVAREZ-VALLINA, L. ET AL.: "antigen-specific targeting of CD28-mediated T cell co-stimulation using chimeric single-chain antibody variable fragment-CD28 receptors", EUR. J. IMMUNOL, vol. 26, no. 10, 1996, pages 2304 - 2309, XP000646504
MASASHIRO KAWAHARA ET AL.: "Kotai engineering no Saizensen Kotai o mochiita Juyotai no engineering-kokateki na saibo Iryo o Mezashite-,", BIO. IND., vol. 20, no. 7, July 2003 (2003-07-01), pages 23 - 33, XP002977427
FITZER-ATTAS, C. J. ET AL.: "harnessing Syk family tyrosine kinases as signaling domains for chimeric single chain of the variable domain receptors: optimal design for T cell activation", J. IMMUNOL.,, vol. 160, no. 1, 1998, pages 145 - 154, XP002977425
FITZER-ATTAS, C. J. ET AL.: "direct T cell activation by chimeric single chain Fv-Syk promotes Syk-Cbl association and Cbl phosphorylation", J. BIOL. CHEM, vol. 272, no. 13, 1997, pages 8551 - 8557, XP002977426
ALVAREZ-VALLINA, L. ET AL.: "antigen-specific targeting of CD28-mediated T cell co-stimulation using chimeric single-chain antibody variable fragment-CD28 receptors", EUR. J. IMMUNOL, vol. 26, no. 10, 1996, pages 2304 - 2309, XP000646504
MASASHIRO KAWAHARA ET AL.: "Kotai engineering no Saizensen Kotai o mochiita Juyotai no engineering-kokateki na saibo Iryo o Mezashite-,", BIO. IND., vol. 20, no. 7, July 2003 (2003-07-01), pages 23 - 33, XP002977427
Attorney, Agent or Firm:
Hirota, Masanori (8-5 Akasaka 2-chom, Minato-ku Tokyo, JP)
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