Title:
ANTEROGRADE MULTI-SYNAPTIC TRANSNEURONAL TRACER
Document Type and Number:
WIPO Patent Application WO/2018/082091
Kind Code:
A1
Abstract:
Provided is a recombinant Herpes Simplex Virus type 1 (HSV-1) strain H129-derived anterograde multi-synaptic transneuronal viral tracer for mutli-synaptic neural circuit mapping, which comprises two or more fluorescence expression cassettes being integrated into the H129 genome at different locations; wherein each fluorescence expression cassette contains at least two copies of fluorescent protein-encoding sequence that are arranged in tandem, and at least one linker-encoding sequence, where at least one linker-encoding sequence is disposed between two fluorescent protein-encoding sequences, allowing transcription of fluorescent protein-encoding sequences and linker-encoding sequence as a single transcript; and wherein the linker-encoding sequence encodes a linker peptide containing at least two adjacent amino acids that are highly inefficient in forming a peptide bond between them; thereby, when the single transcript is translated, at least two fluorescent proteins are stoichiometrically generated due to the impedence of peptide bond formation by the linker peptide.
Inventors:
LUO MINHUA (CN)
ZENG WENBO (CN)
JIANG HAIFEI (CN)
ZHAO FEI (CN)
YANG HONG (CN)
SONG YIGE (CN)
SHEN ZHANGZHOU (CN)
ZENG WENBO (CN)
JIANG HAIFEI (CN)
ZHAO FEI (CN)
YANG HONG (CN)
SONG YIGE (CN)
SHEN ZHANGZHOU (CN)
Application Number:
PCT/CN2016/104880
Publication Date:
May 11, 2018
Filing Date:
November 07, 2016
Export Citation:
Assignee:
WUHAN INST VIROLOGY CAS (CN)
International Classes:
C12N7/01
Domestic Patent References:
| WO2003020898A2 | 2003-03-13 | |||
| WO2007055823A2 | 2007-05-18 |
Foreign References:
| CN104099357A | 2014-10-15 |
Other References:
IAN B. HOGUE ET AL.: "Fluorescent protein approaches in alpha herpesvirus research", VIRUSES, vol. 7, no. 12, 19 November 2015 (2015-11-19), XP055401904, ISSN: 1999-4915
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
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