Title:
ANTI-GHRELIN FAB ANTIBODIES
Document Type and Number:
WIPO Patent Application WO2005016951
Kind Code:
A3
Abstract:
Monoclonal antibodies, including humanized antibodies, which preferentially bind acetylated human ghrelin with respect to unacylated human ghrelin are disclosed. These antibodies have high affinity for acylated human ghrelin, a slow off rate for acylated human ghrelin dissociation and neutralize an acylated human ghrelin activity. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antigen-binding portions, of the invention are useful for neutralizing ghrelin activity, e.g., in a human subject suffering from a disorder in which ghrelin activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant anti-ghrelin antibodies are also encompassed by the invention.
Inventors:
HEIMAN MARK LOUIS (US)
KIKLY KRISTINE KAY (US)
MANETTA JOSEPH VINCENT (US)
WITCHER DERRICK RYAN (US)
KIKLY KRISTINE KAY (US)
MANETTA JOSEPH VINCENT (US)
WITCHER DERRICK RYAN (US)
Application Number:
PCT/US2004/014537
Publication Date:
May 26, 2005
Filing Date:
May 28, 2004
Export Citation:
Assignee:
LILLY CO ELI (US)
HEIMAN MARK LOUIS (US)
KIKLY KRISTINE KAY (US)
MANETTA JOSEPH VINCENT (US)
WITCHER DERRICK RYAN (US)
HEIMAN MARK LOUIS (US)
KIKLY KRISTINE KAY (US)
MANETTA JOSEPH VINCENT (US)
WITCHER DERRICK RYAN (US)
International Classes:
C07K16/26; (IPC1-7): A61K39/395; A61P3/06
Foreign References:
| EP1197496A1 | 2002-04-17 |
Other References:
HOSODA H ET AL: "STRUCTURAL DIVERGENCE OF HUMAN GHRELIN IDENTIFICATION OF MULTIPLE GHRELIN-DERIVED MOLECULES PRODUCED BY POST-TRANSLATIONAL PROCESSING", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 278, no. 1, 3 January 2003 (2003-01-03), pages 64 - 70, XP001205462, ISSN: 0021-9258
MURAKAMI N ET AL: "Role for central ghrelin in food intake and secretion profile of stomach ghrelin in rats", JOURNAL OF ENDOCRINOLOGY, BRISTOL, GB, vol. 174, no. 2, August 2002 (2002-08-01), pages 283 - 288, XP009022559, ISSN: 0022-0795
DAVIES J ET AL: "Affinity improvement of single antibody VH domains: residues in all three hypervariable regions affect antigen binding", IMMUNOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS BV, NL, vol. 2, no. 3, September 1996 (1996-09-01), pages 169 - 179, XP004070292, ISSN: 1380-2933
HOLT L J ET AL: "Domain antibodies: proteins for therapy", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 21, no. 11, November 2003 (2003-11-01), pages 484 - 490, XP004467495, ISSN: 0167-7799
See also references of EP 1633397A2
MURAKAMI N ET AL: "Role for central ghrelin in food intake and secretion profile of stomach ghrelin in rats", JOURNAL OF ENDOCRINOLOGY, BRISTOL, GB, vol. 174, no. 2, August 2002 (2002-08-01), pages 283 - 288, XP009022559, ISSN: 0022-0795
DAVIES J ET AL: "Affinity improvement of single antibody VH domains: residues in all three hypervariable regions affect antigen binding", IMMUNOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS BV, NL, vol. 2, no. 3, September 1996 (1996-09-01), pages 169 - 179, XP004070292, ISSN: 1380-2933
HOLT L J ET AL: "Domain antibodies: proteins for therapy", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 21, no. 11, November 2003 (2003-11-01), pages 484 - 490, XP004467495, ISSN: 0167-7799
See also references of EP 1633397A2
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